Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02035 and BPHYT_RS02040 are separated by 80 nucleotides BPHYT_RS02040 and BPHYT_RS02045 are separated by 121 nucleotides BPHYT_RS02045 and BPHYT_RS02050 overlap by 4 nucleotides
BPHYT_RS02035: BPHYT_RS02035 - hypothetical protein, at 466,452 to 466,697
_RS02035
BPHYT_RS02040: BPHYT_RS02040 - hypothetical protein, at 466,778 to 467,008
_RS02040
BPHYT_RS02045: BPHYT_RS02045 - Fructokinase (EC 2.7.1.4) <small>(from data)</small>, at 467,130 to 468,047
_RS02045
BPHYT_RS02050: BPHYT_RS02050 - D-mannose isomerase (EC 5.3.1.7) <small>(from data)</small>, at 468,044 to 469,327
_RS02050
Position (kb) 467
468
469 Strain fitness (log2 ratio) -2
-1
0
1
2 at 466.306 kb on - strand at 466.472 kb on + strand at 466.473 kb on - strand at 466.666 kb on + strand, within BPHYT_RS02035 at 466.667 kb on - strand, within BPHYT_RS02035 at 466.870 kb on - strand, within BPHYT_RS02040 at 466.977 kb on + strand, within BPHYT_RS02040 at 466.978 kb on - strand, within BPHYT_RS02040 at 466.978 kb on - strand, within BPHYT_RS02040 at 466.992 kb on + strand at 466.993 kb on - strand at 467.049 kb on + strand at 467.050 kb on - strand at 467.050 kb on - strand at 467.301 kb on - strand, within BPHYT_RS02045 at 467.528 kb on - strand, within BPHYT_RS02045 at 467.645 kb on + strand, within BPHYT_RS02045 at 467.645 kb on + strand, within BPHYT_RS02045 at 467.743 kb on + strand, within BPHYT_RS02045 at 468.114 kb on + strand at 468.172 kb on - strand at 468.172 kb on - strand at 468.342 kb on + strand, within BPHYT_RS02050 at 468.342 kb on + strand, within BPHYT_RS02050 at 468.342 kb on + strand, within BPHYT_RS02050 at 468.342 kb on + strand, within BPHYT_RS02050 at 468.343 kb on - strand, within BPHYT_RS02050 at 468.384 kb on + strand, within BPHYT_RS02050 at 468.385 kb on - strand, within BPHYT_RS02050 at 468.385 kb on - strand, within BPHYT_RS02050 at 468.387 kb on + strand, within BPHYT_RS02050 at 468.388 kb on - strand, within BPHYT_RS02050 at 468.573 kb on + strand, within BPHYT_RS02050 at 468.573 kb on + strand, within BPHYT_RS02050 at 468.652 kb on + strand, within BPHYT_RS02050 at 468.685 kb on - strand, within BPHYT_RS02050 at 468.685 kb on - strand, within BPHYT_RS02050 at 468.990 kb on + strand, within BPHYT_RS02050
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 466,306 - -2.4 466,472 + +1.0 466,473 - +0.3 466,666 + BPHYT_RS02035 0.87 +0.4 466,667 - BPHYT_RS02035 0.87 +1.7 466,870 - BPHYT_RS02040 0.40 -1.2 466,977 + BPHYT_RS02040 0.86 +1.5 466,978 - BPHYT_RS02040 0.87 -2.1 466,978 - BPHYT_RS02040 0.87 +1.1 466,992 + +0.2 466,993 - +2.1 467,049 + +0.1 467,050 - -1.0 467,050 - +2.3 467,301 - BPHYT_RS02045 0.19 -1.5 467,528 - BPHYT_RS02045 0.43 -0.9 467,645 + BPHYT_RS02045 0.56 -1.5 467,645 + BPHYT_RS02045 0.56 -1.3 467,743 + BPHYT_RS02045 0.67 -2.5 468,114 + -0.1 468,172 - -1.5 468,172 - -0.7 468,342 + BPHYT_RS02050 0.23 -1.1 468,342 + BPHYT_RS02050 0.23 -0.5 468,342 + BPHYT_RS02050 0.23 -0.2 468,342 + BPHYT_RS02050 0.23 -1.4 468,343 - BPHYT_RS02050 0.23 -0.9 468,384 + BPHYT_RS02050 0.26 +0.1 468,385 - BPHYT_RS02050 0.27 -0.3 468,385 - BPHYT_RS02050 0.27 -0.1 468,387 + BPHYT_RS02050 0.27 -0.5 468,388 - BPHYT_RS02050 0.27 -0.3 468,573 + BPHYT_RS02050 0.41 -0.6 468,573 + BPHYT_RS02050 0.41 -1.2 468,652 + BPHYT_RS02050 0.47 +0.2 468,685 - BPHYT_RS02050 0.50 +1.6 468,685 - BPHYT_RS02050 0.50 -0.0 468,990 + BPHYT_RS02050 0.74 +1.0
Or see this region's nucleotide sequence