Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01955 and BPHYT_RS01960 overlap by 1 nucleotides BPHYT_RS01960 and BPHYT_RS01965 are separated by 77 nucleotides BPHYT_RS01965 and BPHYT_RS01970 are separated by 149 nucleotides BPHYT_RS01970 and BPHYT_RS01975 are separated by 248 nucleotides BPHYT_RS01975 and BPHYT_RS01980 are separated by 165 nucleotides
BPHYT_RS01955: BPHYT_RS01955 - hypothetical protein, at 442,953 to 443,750
_RS01955
BPHYT_RS01960: BPHYT_RS01960 - cytochrome C, at 443,750 to 444,097
_RS01960
BPHYT_RS01965: BPHYT_RS01965 - tRNA-Ala, at 444,175 to 444,250
_RS01965
BPHYT_RS01970: BPHYT_RS01970 - methyl-accepting chemotaxis protein, at 444,400 to 446,157
_RS01970
BPHYT_RS01975: BPHYT_RS01975 - hypothetical protein, at 446,406 to 446,639
_RS01975
BPHYT_RS01980: BPHYT_RS01980 - diguanylate cyclase, at 446,805 to 448,337
_RS01980
Position (kb) 444
445
446
447 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 443.446 kb on - strand, within BPHYT_RS01955 at 443.542 kb on - strand, within BPHYT_RS01955 at 443.542 kb on - strand, within BPHYT_RS01955 at 443.542 kb on - strand, within BPHYT_RS01955 at 443.936 kb on + strand, within BPHYT_RS01960 at 443.936 kb on + strand, within BPHYT_RS01960 at 443.936 kb on + strand, within BPHYT_RS01960 at 443.937 kb on - strand, within BPHYT_RS01960 at 444.156 kb on + strand at 444.182 kb on + strand at 444.182 kb on + strand at 444.183 kb on - strand, within BPHYT_RS01965 at 444.281 kb on - strand at 444.321 kb on + strand at 445.086 kb on + strand, within BPHYT_RS01970 at 445.086 kb on + strand, within BPHYT_RS01970 at 445.087 kb on - strand, within BPHYT_RS01970 at 445.589 kb on + strand, within BPHYT_RS01970 at 445.601 kb on + strand, within BPHYT_RS01970 at 445.602 kb on - strand, within BPHYT_RS01970 at 445.629 kb on + strand, within BPHYT_RS01970 at 445.769 kb on + strand, within BPHYT_RS01970 at 445.769 kb on + strand, within BPHYT_RS01970 at 445.770 kb on - strand, within BPHYT_RS01970 at 445.909 kb on + strand, within BPHYT_RS01970 at 445.909 kb on + strand, within BPHYT_RS01970 at 445.948 kb on - strand, within BPHYT_RS01970 at 446.055 kb on + strand at 446.095 kb on - strand at 446.239 kb on + strand at 446.264 kb on + strand at 446.264 kb on + strand at 446.264 kb on + strand at 446.383 kb on + strand at 446.383 kb on + strand at 446.383 kb on + strand at 446.384 kb on - strand at 446.384 kb on - strand at 446.437 kb on + strand, within BPHYT_RS01975 at 446.776 kb on + strand at 446.843 kb on + strand at 446.844 kb on - strand at 446.985 kb on + strand, within BPHYT_RS01980 at 446.986 kb on - strand, within BPHYT_RS01980 at 446.986 kb on - strand, within BPHYT_RS01980
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 443,446 - BPHYT_RS01955 0.62 -3.6 443,542 - BPHYT_RS01955 0.74 -1.9 443,542 - BPHYT_RS01955 0.74 -2.0 443,542 - BPHYT_RS01955 0.74 -2.3 443,936 + BPHYT_RS01960 0.53 -2.8 443,936 + BPHYT_RS01960 0.53 -2.8 443,936 + BPHYT_RS01960 0.53 -2.2 443,937 - BPHYT_RS01960 0.54 -1.9 444,156 + -0.4 444,182 + +0.6 444,182 + +0.2 444,183 - BPHYT_RS01965 0.11 +0.7 444,281 - +1.9 444,321 + -0.1 445,086 + BPHYT_RS01970 0.39 -1.5 445,086 + BPHYT_RS01970 0.39 +0.2 445,087 - BPHYT_RS01970 0.39 +0.1 445,589 + BPHYT_RS01970 0.68 -1.2 445,601 + BPHYT_RS01970 0.68 +0.4 445,602 - BPHYT_RS01970 0.68 -0.1 445,629 + BPHYT_RS01970 0.70 -0.5 445,769 + BPHYT_RS01970 0.78 +0.8 445,769 + BPHYT_RS01970 0.78 +2.0 445,770 - BPHYT_RS01970 0.78 -0.4 445,909 + BPHYT_RS01970 0.86 -0.2 445,909 + BPHYT_RS01970 0.86 +0.5 445,948 - BPHYT_RS01970 0.88 +0.6 446,055 + -0.5 446,095 - +0.6 446,239 + -0.6 446,264 + -1.9 446,264 + +0.8 446,264 + -0.1 446,383 + +1.9 446,383 + +0.4 446,383 + +1.1 446,384 - +2.9 446,384 - -1.0 446,437 + BPHYT_RS01975 0.13 +1.3 446,776 + -0.3 446,843 + +1.6 446,844 - -0.5 446,985 + BPHYT_RS01980 0.12 +0.1 446,986 - BPHYT_RS01980 0.12 -1.5 446,986 - BPHYT_RS01980 0.12 -0.1
Or see this region's nucleotide sequence