Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS01885

Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS01880 and BPHYT_RS01885 are separated by 423 nucleotidesBPHYT_RS01885 and BPHYT_RS01890 are separated by 132 nucleotides BPHYT_RS01880: BPHYT_RS01880 - nitronate monooxygenase, at 427,454 to 428,554 _RS01880 BPHYT_RS01885: BPHYT_RS01885 - branched-chain amino acid ABC transporter substrate-binding protein, at 428,978 to 430,123 _RS01885 BPHYT_RS01890: BPHYT_RS01890 - cupin, at 430,256 to 431,272 _RS01890 Position (kb) 428 429 430 431Strain fitness (log2 ratio) -2 -1 0 1 2 3at 428.298 kb on + strand, within BPHYT_RS01880at 428.417 kb on + strand, within BPHYT_RS01880at 428.417 kb on + strand, within BPHYT_RS01880at 428.417 kb on + strand, within BPHYT_RS01880at 428.417 kb on + strand, within BPHYT_RS01880at 428.418 kb on - strand, within BPHYT_RS01880at 428.427 kb on + strand, within BPHYT_RS01880at 428.621 kb on - strandat 428.636 kb on + strandat 428.749 kb on + strandat 428.749 kb on + strandat 428.752 kb on + strandat 428.752 kb on + strandat 428.752 kb on + strandat 428.752 kb on + strandat 428.803 kb on + strandat 428.804 kb on - strandat 428.804 kb on - strandat 428.819 kb on + strandat 428.944 kb on + strandat 428.945 kb on - strandat 428.975 kb on + strandat 428.975 kb on + strandat 428.975 kb on + strandat 428.975 kb on + strandat 429.335 kb on + strand, within BPHYT_RS01885at 429.335 kb on + strand, within BPHYT_RS01885at 429.335 kb on + strand, within BPHYT_RS01885at 429.335 kb on + strand, within BPHYT_RS01885at 429.336 kb on - strandat 429.336 kb on - strand, within BPHYT_RS01885at 429.337 kb on + strand, within BPHYT_RS01885at 429.461 kb on + strand, within BPHYT_RS01885at 429.461 kb on + strand, within BPHYT_RS01885at 429.665 kb on + strand, within BPHYT_RS01885at 429.666 kb on - strand, within BPHYT_RS01885at 429.666 kb on - strand, within BPHYT_RS01885at 429.866 kb on + strand, within BPHYT_RS01885at 429.866 kb on + strand, within BPHYT_RS01885at 429.867 kb on - strand, within BPHYT_RS01885at 429.900 kb on - strand, within BPHYT_RS01885at 429.900 kb on - strand, within BPHYT_RS01885at 429.915 kb on - strand, within BPHYT_RS01885at 429.915 kb on - strand, within BPHYT_RS01885at 429.926 kb on + strand, within BPHYT_RS01885at 430.004 kb on + strand, within BPHYT_RS01885at 430.005 kb on - strand, within BPHYT_RS01885at 430.074 kb on - strandat 430.079 kb on + strandat 430.079 kb on + strandat 430.080 kb on - strandat 430.080 kb on - strandat 430.080 kb on - strandat 430.080 kb on - strandat 430.121 kb on + strandat 430.173 kb on + strandat 430.482 kb on - strand, within BPHYT_RS01890at 430.498 kb on + strandat 430.499 kb on - strand, within BPHYT_RS01890at 430.499 kb on - strand, within BPHYT_RS01890at 430.566 kb on - strand, within BPHYT_RS01890at 430.569 kb on - strand, within BPHYT_RS01890at 430.569 kb on - strand, within BPHYT_RS01890at 430.644 kb on - strand, within BPHYT_RS01890at 430.757 kb on + strand, within BPHYT_RS01890at 430.800 kb on - strand, within BPHYT_RS01890at 430.800 kb on - strand, within BPHYT_RS01890at 430.813 kb on + strand, within BPHYT_RS01890at 430.877 kb on + strand, within BPHYT_RS01890at 430.877 kb on + strand, within BPHYT_RS01890at 431.030 kb on - strand, within BPHYT_RS01890

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Per-strain Table

Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
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428,298 + BPHYT_RS01880 0.77 +0.7
428,417 + BPHYT_RS01880 0.87 -1.5
428,417 + BPHYT_RS01880 0.87 -1.1
428,417 + BPHYT_RS01880 0.87 +1.5
428,417 + BPHYT_RS01880 0.87 -0.9
428,418 - BPHYT_RS01880 0.88 +1.7
428,427 + BPHYT_RS01880 0.88 +0.2
428,621 - -0.8
428,636 + +0.0
428,749 + -0.3
428,749 + +1.3
428,752 + -0.3
428,752 + -1.6
428,752 + +0.6
428,752 + -0.5
428,803 + -1.0
428,804 - +0.8
428,804 - -0.9
428,819 + -0.4
428,944 + +0.2
428,945 - +1.4
428,975 + -1.8
428,975 + -0.4
428,975 + -0.6
428,975 + -0.1
429,335 + BPHYT_RS01885 0.31 -2.6
429,335 + BPHYT_RS01885 0.31 -1.9
429,335 + BPHYT_RS01885 0.31 -1.2
429,335 + BPHYT_RS01885 0.31 -1.8
429,336 - +0.9
429,336 - BPHYT_RS01885 0.31 -1.1
429,337 + BPHYT_RS01885 0.31 +0.1
429,461 + BPHYT_RS01885 0.42 -1.6
429,461 + BPHYT_RS01885 0.42 -1.4
429,665 + BPHYT_RS01885 0.60 -1.9
429,666 - BPHYT_RS01885 0.60 -1.3
429,666 - BPHYT_RS01885 0.60 -0.1
429,866 + BPHYT_RS01885 0.77 -1.6
429,866 + BPHYT_RS01885 0.77 -0.9
429,867 - BPHYT_RS01885 0.78 -1.6
429,900 - BPHYT_RS01885 0.80 -1.8
429,900 - BPHYT_RS01885 0.80 -0.9
429,915 - BPHYT_RS01885 0.82 -2.1
429,915 - BPHYT_RS01885 0.82 -1.5
429,926 + BPHYT_RS01885 0.83 -0.3
430,004 + BPHYT_RS01885 0.90 -0.6
430,005 - BPHYT_RS01885 0.90 -1.4
430,074 - -1.1
430,079 + +3.4
430,079 + -0.4
430,080 - -1.0
430,080 - -1.8
430,080 - -1.4
430,080 - -1.6
430,121 + +1.0
430,173 + +1.3
430,482 - BPHYT_RS01890 0.22 -0.7
430,498 + +0.7
430,499 - BPHYT_RS01890 0.24 +1.2
430,499 - BPHYT_RS01890 0.24 -0.0
430,566 - BPHYT_RS01890 0.30 +0.6
430,569 - BPHYT_RS01890 0.31 +0.1
430,569 - BPHYT_RS01890 0.31 -0.5
430,644 - BPHYT_RS01890 0.38 +0.5
430,757 + BPHYT_RS01890 0.49 +1.0
430,800 - BPHYT_RS01890 0.53 -0.9
430,800 - BPHYT_RS01890 0.53 +2.3
430,813 + BPHYT_RS01890 0.55 +1.3
430,877 + BPHYT_RS01890 0.61 +0.2
430,877 + BPHYT_RS01890 0.61 +0.0
431,030 - BPHYT_RS01890 0.76 -0.4

Or see this region's nucleotide sequence