Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01855 and BPHYT_RS01860 are separated by 278 nucleotides BPHYT_RS01860 and BPHYT_RS01865 are separated by 235 nucleotides
BPHYT_RS01855: BPHYT_RS01855 - FAD-dependent oxidoreductase, at 421,365 to 422,471
_RS01855
BPHYT_RS01860: BPHYT_RS01860 - high potential iron-sulfur protein, at 422,750 to 423,061
_RS01860
BPHYT_RS01865: BPHYT_RS01865 - major facilitator transporter, at 423,297 to 424,589
_RS01865
Position (kb) 422
423
424 Strain fitness (log2 ratio) -2
-1
0
1
2 at 422.058 kb on + strand, within BPHYT_RS01855 at 422.058 kb on + strand, within BPHYT_RS01855 at 422.100 kb on + strand, within BPHYT_RS01855 at 422.202 kb on + strand, within BPHYT_RS01855 at 422.203 kb on - strand, within BPHYT_RS01855 at 422.203 kb on - strand, within BPHYT_RS01855 at 422.235 kb on + strand, within BPHYT_RS01855 at 422.254 kb on - strand, within BPHYT_RS01855 at 422.315 kb on - strand, within BPHYT_RS01855 at 422.647 kb on - strand at 422.689 kb on - strand at 422.731 kb on - strand at 422.731 kb on - strand at 422.732 kb on + strand at 422.880 kb on - strand, within BPHYT_RS01860 at 422.924 kb on + strand, within BPHYT_RS01860 at 422.925 kb on - strand, within BPHYT_RS01860 at 422.953 kb on + strand, within BPHYT_RS01860 at 422.954 kb on - strand, within BPHYT_RS01860 at 422.954 kb on - strand, within BPHYT_RS01860 at 422.960 kb on + strand, within BPHYT_RS01860 at 423.044 kb on + strand at 423.044 kb on + strand at 423.045 kb on - strand at 423.047 kb on - strand at 423.206 kb on - strand at 423.253 kb on - strand at 423.265 kb on + strand at 423.266 kb on - strand at 423.266 kb on - strand at 423.272 kb on + strand at 423.370 kb on - strand at 423.509 kb on - strand, within BPHYT_RS01865 at 423.519 kb on + strand, within BPHYT_RS01865 at 423.520 kb on - strand, within BPHYT_RS01865 at 423.647 kb on - strand, within BPHYT_RS01865 at 423.900 kb on + strand, within BPHYT_RS01865 at 423.901 kb on - strand, within BPHYT_RS01865 at 424.047 kb on + strand, within BPHYT_RS01865 at 424.047 kb on + strand, within BPHYT_RS01865 at 424.048 kb on - strand, within BPHYT_RS01865
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 422,058 + BPHYT_RS01855 0.63 +2.5 422,058 + BPHYT_RS01855 0.63 +1.1 422,100 + BPHYT_RS01855 0.66 +0.5 422,202 + BPHYT_RS01855 0.76 +1.2 422,203 - BPHYT_RS01855 0.76 -0.9 422,203 - BPHYT_RS01855 0.76 -0.5 422,235 + BPHYT_RS01855 0.79 -2.0 422,254 - BPHYT_RS01855 0.80 -1.9 422,315 - BPHYT_RS01855 0.86 -0.0 422,647 - +2.2 422,689 - -0.2 422,731 - -1.1 422,731 - -2.4 422,732 + +0.4 422,880 - BPHYT_RS01860 0.42 +0.0 422,924 + BPHYT_RS01860 0.56 -0.8 422,925 - BPHYT_RS01860 0.56 -1.6 422,953 + BPHYT_RS01860 0.65 +0.6 422,954 - BPHYT_RS01860 0.65 +1.1 422,954 - BPHYT_RS01860 0.65 -0.1 422,960 + BPHYT_RS01860 0.67 -1.3 423,044 + +0.0 423,044 + -0.3 423,045 - -1.9 423,047 - -0.6 423,206 - -0.3 423,253 - +0.2 423,265 + -1.9 423,266 - -0.8 423,266 - +0.4 423,272 + +0.3 423,370 - -1.1 423,509 - BPHYT_RS01865 0.16 -0.7 423,519 + BPHYT_RS01865 0.17 -1.6 423,520 - BPHYT_RS01865 0.17 +0.0 423,647 - BPHYT_RS01865 0.27 -1.1 423,900 + BPHYT_RS01865 0.47 -1.5 423,901 - BPHYT_RS01865 0.47 -0.1 424,047 + BPHYT_RS01865 0.58 +0.5 424,047 + BPHYT_RS01865 0.58 +0.4 424,048 - BPHYT_RS01865 0.58 -0.6
Or see this region's nucleotide sequence