Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01850 and BPHYT_RS01855 are separated by 418 nucleotides BPHYT_RS01855 and BPHYT_RS01860 are separated by 278 nucleotides BPHYT_RS01860 and BPHYT_RS01865 are separated by 235 nucleotides
BPHYT_RS01850: BPHYT_RS01850 - ATPase AAA, at 419,969 to 420,946
_RS01850
BPHYT_RS01855: BPHYT_RS01855 - FAD-dependent oxidoreductase, at 421,365 to 422,471
_RS01855
BPHYT_RS01860: BPHYT_RS01860 - high potential iron-sulfur protein, at 422,750 to 423,061
_RS01860
BPHYT_RS01865: BPHYT_RS01865 - major facilitator transporter, at 423,297 to 424,589
_RS01865
Position (kb) 421
422
423 Strain fitness (log2 ratio) -2
-1
0
1
2 at 420.455 kb on + strand, within BPHYT_RS01850 at 420.456 kb on - strand, within BPHYT_RS01850 at 420.512 kb on + strand, within BPHYT_RS01850 at 420.512 kb on + strand, within BPHYT_RS01850 at 420.512 kb on + strand, within BPHYT_RS01850 at 420.512 kb on + strand, within BPHYT_RS01850 at 420.513 kb on - strand, within BPHYT_RS01850 at 420.513 kb on - strand, within BPHYT_RS01850 at 420.513 kb on - strand, within BPHYT_RS01850 at 420.533 kb on + strand, within BPHYT_RS01850 at 420.533 kb on + strand, within BPHYT_RS01850 at 420.533 kb on + strand, within BPHYT_RS01850 at 420.533 kb on + strand, within BPHYT_RS01850 at 420.533 kb on + strand, within BPHYT_RS01850 at 420.534 kb on - strand, within BPHYT_RS01850 at 420.534 kb on - strand, within BPHYT_RS01850 at 420.534 kb on - strand, within BPHYT_RS01850 at 420.677 kb on + strand, within BPHYT_RS01850 at 420.677 kb on + strand, within BPHYT_RS01850 at 420.677 kb on + strand, within BPHYT_RS01850 at 420.677 kb on + strand, within BPHYT_RS01850 at 420.678 kb on - strand, within BPHYT_RS01850 at 420.678 kb on - strand, within BPHYT_RS01850 at 420.678 kb on - strand, within BPHYT_RS01850 at 420.731 kb on + strand, within BPHYT_RS01850 at 420.754 kb on + strand, within BPHYT_RS01850 at 420.755 kb on - strand, within BPHYT_RS01850 at 420.995 kb on - strand at 421.047 kb on - strand at 421.096 kb on + strand at 421.096 kb on + strand at 421.097 kb on - strand at 421.098 kb on - strand at 421.131 kb on - strand at 421.131 kb on - strand at 421.156 kb on + strand at 421.157 kb on - strand at 421.157 kb on - strand at 421.157 kb on - strand at 421.217 kb on + strand at 421.281 kb on + strand at 421.282 kb on - strand at 421.384 kb on + strand at 421.384 kb on + strand at 421.527 kb on + strand, within BPHYT_RS01855 at 421.527 kb on + strand, within BPHYT_RS01855 at 421.528 kb on - strand, within BPHYT_RS01855 at 421.528 kb on - strand, within BPHYT_RS01855 at 421.528 kb on - strand, within BPHYT_RS01855 at 421.528 kb on - strand, within BPHYT_RS01855 at 421.528 kb on - strand, within BPHYT_RS01855 at 421.528 kb on - strand, within BPHYT_RS01855 at 421.528 kb on - strand, within BPHYT_RS01855 at 421.584 kb on + strand, within BPHYT_RS01855 at 421.585 kb on - strand, within BPHYT_RS01855 at 421.585 kb on - strand, within BPHYT_RS01855 at 421.585 kb on - strand, within BPHYT_RS01855 at 421.590 kb on + strand, within BPHYT_RS01855 at 421.591 kb on - strand, within BPHYT_RS01855 at 422.058 kb on + strand, within BPHYT_RS01855 at 422.058 kb on + strand, within BPHYT_RS01855 at 422.100 kb on + strand, within BPHYT_RS01855 at 422.202 kb on + strand, within BPHYT_RS01855 at 422.203 kb on - strand, within BPHYT_RS01855 at 422.203 kb on - strand, within BPHYT_RS01855 at 422.235 kb on + strand, within BPHYT_RS01855 at 422.254 kb on - strand, within BPHYT_RS01855 at 422.315 kb on - strand, within BPHYT_RS01855 at 422.647 kb on - strand at 422.689 kb on - strand at 422.731 kb on - strand at 422.731 kb on - strand at 422.732 kb on + strand at 422.880 kb on - strand, within BPHYT_RS01860 at 422.924 kb on + strand, within BPHYT_RS01860 at 422.925 kb on - strand, within BPHYT_RS01860 at 422.953 kb on + strand, within BPHYT_RS01860 at 422.954 kb on - strand, within BPHYT_RS01860 at 422.954 kb on - strand, within BPHYT_RS01860 at 422.960 kb on + strand, within BPHYT_RS01860 at 423.044 kb on + strand at 423.044 kb on + strand at 423.045 kb on - strand at 423.047 kb on - strand at 423.206 kb on - strand at 423.253 kb on - strand at 423.265 kb on + strand at 423.266 kb on - strand at 423.266 kb on - strand at 423.272 kb on + strand at 423.370 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 420,455 + BPHYT_RS01850 0.50 -2.1 420,456 - BPHYT_RS01850 0.50 -0.3 420,512 + BPHYT_RS01850 0.56 -2.0 420,512 + BPHYT_RS01850 0.56 -0.0 420,512 + BPHYT_RS01850 0.56 +0.2 420,512 + BPHYT_RS01850 0.56 +0.3 420,513 - BPHYT_RS01850 0.56 -0.2 420,513 - BPHYT_RS01850 0.56 +0.2 420,513 - BPHYT_RS01850 0.56 +0.0 420,533 + BPHYT_RS01850 0.58 -0.3 420,533 + BPHYT_RS01850 0.58 +2.2 420,533 + BPHYT_RS01850 0.58 -0.2 420,533 + BPHYT_RS01850 0.58 +0.6 420,533 + BPHYT_RS01850 0.58 -0.2 420,534 - BPHYT_RS01850 0.58 -0.5 420,534 - BPHYT_RS01850 0.58 +0.4 420,534 - BPHYT_RS01850 0.58 -1.1 420,677 + BPHYT_RS01850 0.72 +0.3 420,677 + BPHYT_RS01850 0.72 -0.0 420,677 + BPHYT_RS01850 0.72 -1.9 420,677 + BPHYT_RS01850 0.72 -0.6 420,678 - BPHYT_RS01850 0.72 +0.1 420,678 - BPHYT_RS01850 0.72 -0.7 420,678 - BPHYT_RS01850 0.72 -0.0 420,731 + BPHYT_RS01850 0.78 -0.4 420,754 + BPHYT_RS01850 0.80 +0.7 420,755 - BPHYT_RS01850 0.80 +1.1 420,995 - +0.2 421,047 - -1.4 421,096 + +0.5 421,096 + +1.3 421,097 - -0.0 421,098 - +0.6 421,131 - +0.7 421,131 - +0.7 421,156 + +1.2 421,157 - +0.6 421,157 - +1.0 421,157 - -0.2 421,217 + -1.6 421,281 + +1.1 421,282 - -1.2 421,384 + -0.1 421,384 + +0.1 421,527 + BPHYT_RS01855 0.15 -0.6 421,527 + BPHYT_RS01855 0.15 -0.0 421,528 - BPHYT_RS01855 0.15 -0.2 421,528 - BPHYT_RS01855 0.15 -1.1 421,528 - BPHYT_RS01855 0.15 -2.5 421,528 - BPHYT_RS01855 0.15 +0.4 421,528 - BPHYT_RS01855 0.15 +0.3 421,528 - BPHYT_RS01855 0.15 +1.0 421,528 - BPHYT_RS01855 0.15 -0.1 421,584 + BPHYT_RS01855 0.20 +0.8 421,585 - BPHYT_RS01855 0.20 +0.7 421,585 - BPHYT_RS01855 0.20 -0.2 421,585 - BPHYT_RS01855 0.20 -0.2 421,590 + BPHYT_RS01855 0.20 +0.7 421,591 - BPHYT_RS01855 0.20 +1.4 422,058 + BPHYT_RS01855 0.63 +2.5 422,058 + BPHYT_RS01855 0.63 +1.1 422,100 + BPHYT_RS01855 0.66 +0.5 422,202 + BPHYT_RS01855 0.76 +1.2 422,203 - BPHYT_RS01855 0.76 -0.9 422,203 - BPHYT_RS01855 0.76 -0.5 422,235 + BPHYT_RS01855 0.79 -2.0 422,254 - BPHYT_RS01855 0.80 -1.9 422,315 - BPHYT_RS01855 0.86 -0.0 422,647 - +2.2 422,689 - -0.2 422,731 - -1.1 422,731 - -2.4 422,732 + +0.4 422,880 - BPHYT_RS01860 0.42 +0.0 422,924 + BPHYT_RS01860 0.56 -0.8 422,925 - BPHYT_RS01860 0.56 -1.6 422,953 + BPHYT_RS01860 0.65 +0.6 422,954 - BPHYT_RS01860 0.65 +1.1 422,954 - BPHYT_RS01860 0.65 -0.1 422,960 + BPHYT_RS01860 0.67 -1.3 423,044 + +0.0 423,044 + -0.3 423,045 - -1.9 423,047 - -0.6 423,206 - -0.3 423,253 - +0.2 423,265 + -1.9 423,266 - -0.8 423,266 - +0.4 423,272 + +0.3 423,370 - -1.1
Or see this region's nucleotide sequence