Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01765 and BPHYT_RS01770 are separated by 10 nucleotides BPHYT_RS01770 and BPHYT_RS01775 are separated by 378 nucleotides BPHYT_RS01775 and BPHYT_RS01780 overlap by 4 nucleotides
BPHYT_RS01765: BPHYT_RS01765 - carbon-monoxide dehydrogenase, at 401,180 to 403,561
_RS01765
BPHYT_RS01770: BPHYT_RS01770 - carbon monoxide dehydrogenase, at 403,572 to 404,090
_RS01770
BPHYT_RS01775: BPHYT_RS01775 - hypothetical protein, at 404,469 to 404,813
_RS01775
BPHYT_RS01780: BPHYT_RS01780 - hypothetical protein, at 404,810 to 405,178
_RS01780
Position (kb) 403
404
405 Strain fitness (log2 ratio) -2
-1
0
1
2 at 402.573 kb on + strand, within BPHYT_RS01765 at 402.574 kb on - strand, within BPHYT_RS01765 at 402.894 kb on + strand, within BPHYT_RS01765 at 402.895 kb on - strand, within BPHYT_RS01765 at 402.903 kb on + strand, within BPHYT_RS01765 at 402.903 kb on + strand, within BPHYT_RS01765 at 402.904 kb on - strand, within BPHYT_RS01765 at 402.924 kb on + strand, within BPHYT_RS01765 at 403.057 kb on - strand, within BPHYT_RS01765 at 403.146 kb on + strand, within BPHYT_RS01765 at 403.365 kb on - strand at 403.479 kb on + strand at 403.506 kb on + strand at 403.507 kb on - strand at 403.516 kb on + strand at 403.516 kb on + strand at 403.516 kb on + strand at 403.517 kb on - strand at 404.202 kb on - strand at 404.226 kb on + strand at 404.305 kb on - strand at 404.307 kb on + strand at 404.307 kb on + strand at 404.308 kb on - strand at 404.335 kb on - strand at 404.406 kb on + strand at 404.425 kb on + strand at 404.425 kb on + strand at 404.425 kb on + strand at 404.425 kb on + strand at 404.426 kb on - strand at 404.470 kb on + strand at 404.588 kb on + strand, within BPHYT_RS01775 at 404.589 kb on - strand, within BPHYT_RS01775 at 404.596 kb on + strand, within BPHYT_RS01775 at 404.597 kb on - strand, within BPHYT_RS01775 at 404.705 kb on - strand, within BPHYT_RS01775 at 404.717 kb on + strand, within BPHYT_RS01775 at 404.718 kb on - strand, within BPHYT_RS01775 at 404.720 kb on + strand, within BPHYT_RS01775 at 404.721 kb on - strand, within BPHYT_RS01775 at 404.721 kb on - strand, within BPHYT_RS01775 at 404.820 kb on - strand at 404.867 kb on + strand, within BPHYT_RS01780 at 404.868 kb on - strand, within BPHYT_RS01780 at 404.868 kb on - strand, within BPHYT_RS01780 at 404.868 kb on - strand, within BPHYT_RS01780 at 404.876 kb on - strand, within BPHYT_RS01780 at 405.024 kb on - strand, within BPHYT_RS01780
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 402,573 + BPHYT_RS01765 0.58 -0.6 402,574 - BPHYT_RS01765 0.59 -1.0 402,894 + BPHYT_RS01765 0.72 +0.4 402,895 - BPHYT_RS01765 0.72 +0.3 402,903 + BPHYT_RS01765 0.72 +0.0 402,903 + BPHYT_RS01765 0.72 -1.1 402,904 - BPHYT_RS01765 0.72 -1.5 402,924 + BPHYT_RS01765 0.73 -0.9 403,057 - BPHYT_RS01765 0.79 +1.1 403,146 + BPHYT_RS01765 0.83 -0.7 403,365 - +0.1 403,479 + -1.9 403,506 + -0.0 403,507 - -0.2 403,516 + +0.0 403,516 + +0.3 403,516 + -1.0 403,517 - -0.2 404,202 - -0.4 404,226 + -0.9 404,305 - +0.5 404,307 + -0.8 404,307 + -0.5 404,308 - -0.2 404,335 - -1.1 404,406 + +0.2 404,425 + -0.7 404,425 + +1.1 404,425 + +0.9 404,425 + -2.5 404,426 - -1.1 404,470 + +0.1 404,588 + BPHYT_RS01775 0.34 +0.2 404,589 - BPHYT_RS01775 0.35 -0.2 404,596 + BPHYT_RS01775 0.37 +0.1 404,597 - BPHYT_RS01775 0.37 +0.2 404,705 - BPHYT_RS01775 0.68 +0.4 404,717 + BPHYT_RS01775 0.72 -0.7 404,718 - BPHYT_RS01775 0.72 +0.6 404,720 + BPHYT_RS01775 0.73 -1.0 404,721 - BPHYT_RS01775 0.73 +0.5 404,721 - BPHYT_RS01775 0.73 -0.8 404,820 - -1.9 404,867 + BPHYT_RS01780 0.15 -0.1 404,868 - BPHYT_RS01780 0.16 -0.9 404,868 - BPHYT_RS01780 0.16 +2.1 404,868 - BPHYT_RS01780 0.16 -0.0 404,876 - BPHYT_RS01780 0.18 +2.0 405,024 - BPHYT_RS01780 0.58 -1.1
Or see this region's nucleotide sequence