Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01680 and BPHYT_RS01685 are separated by 461 nucleotides BPHYT_RS01685 and BPHYT_RS01690 are separated by 89 nucleotides BPHYT_RS01690 and BPHYT_RS01695 are separated by 215 nucleotides
BPHYT_RS01680: BPHYT_RS01680 - hypothetical protein, at 381,442 to 381,807
_RS01680
BPHYT_RS01685: BPHYT_RS01685 - phytanoyl-CoA dioxygenase, at 382,269 to 383,030
_RS01685
BPHYT_RS01690: BPHYT_RS01690 - membrane protein, at 383,120 to 383,326
_RS01690
BPHYT_RS01695: BPHYT_RS01695 - L-glyceraldehyde 3-phosphate reductase, at 383,542 to 384,585
_RS01695
Position (kb) 382
383
384 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 381.434 kb on + strand at 381.435 kb on - strand at 381.435 kb on - strand at 381.443 kb on + strand at 381.801 kb on + strand at 381.801 kb on + strand at 381.801 kb on + strand at 381.801 kb on + strand at 381.802 kb on - strand at 381.802 kb on - strand at 381.802 kb on - strand at 381.820 kb on - strand at 381.820 kb on - strand at 381.933 kb on - strand at 381.933 kb on - strand at 381.960 kb on - strand at 382.007 kb on - strand at 382.007 kb on - strand at 382.027 kb on + strand at 382.027 kb on + strand at 382.028 kb on - strand at 382.061 kb on + strand at 382.170 kb on - strand at 382.170 kb on - strand at 382.170 kb on - strand at 382.176 kb on + strand at 382.176 kb on + strand at 382.176 kb on + strand at 382.176 kb on + strand at 382.177 kb on - strand at 382.177 kb on - strand at 382.265 kb on + strand at 382.265 kb on + strand at 382.266 kb on - strand at 382.266 kb on - strand at 382.266 kb on - strand at 382.266 kb on - strand at 382.266 kb on - strand at 382.434 kb on + strand, within BPHYT_RS01685 at 382.475 kb on + strand, within BPHYT_RS01685 at 382.475 kb on + strand, within BPHYT_RS01685 at 382.475 kb on + strand, within BPHYT_RS01685 at 382.476 kb on - strand, within BPHYT_RS01685 at 382.476 kb on - strand, within BPHYT_RS01685 at 382.476 kb on - strand, within BPHYT_RS01685 at 382.500 kb on + strand, within BPHYT_RS01685 at 382.500 kb on + strand, within BPHYT_RS01685 at 382.500 kb on + strand, within BPHYT_RS01685 at 382.566 kb on + strand, within BPHYT_RS01685 at 382.567 kb on - strand, within BPHYT_RS01685 at 382.567 kb on - strand, within BPHYT_RS01685 at 382.567 kb on - strand, within BPHYT_RS01685 at 382.567 kb on - strand, within BPHYT_RS01685 at 382.605 kb on - strand, within BPHYT_RS01685 at 382.633 kb on - strand, within BPHYT_RS01685 at 382.957 kb on - strand at 382.957 kb on - strand at 382.957 kb on - strand at 382.957 kb on - strand at 382.957 kb on - strand at 382.963 kb on - strand at 382.963 kb on - strand at 383.222 kb on - strand, within BPHYT_RS01690 at 383.274 kb on + strand, within BPHYT_RS01690 at 383.328 kb on + strand at 383.328 kb on + strand at 383.329 kb on - strand at 383.329 kb on - strand at 383.329 kb on - strand at 383.431 kb on + strand at 383.432 kb on - strand at 383.432 kb on - strand at 383.537 kb on + strand at 383.538 kb on - strand at 383.569 kb on + strand at 383.585 kb on - strand at 383.709 kb on + strand, within BPHYT_RS01695 at 383.737 kb on + strand, within BPHYT_RS01695 at 383.737 kb on + strand, within BPHYT_RS01695 at 383.935 kb on + strand, within BPHYT_RS01695 at 383.950 kb on + strand, within BPHYT_RS01695 at 383.951 kb on - strand, within BPHYT_RS01695
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 381,434 + +0.2 381,435 - +1.4 381,435 - +0.2 381,443 + -0.7 381,801 + -2.5 381,801 + -2.2 381,801 + +0.8 381,801 + +0.3 381,802 - -0.0 381,802 - -0.2 381,802 - -1.7 381,820 - -0.2 381,820 - -0.4 381,933 - -0.0 381,933 - -0.3 381,960 - -0.9 382,007 - +0.2 382,007 - -0.2 382,027 + -0.5 382,027 + +0.5 382,028 - +1.3 382,061 + +0.5 382,170 - -0.4 382,170 - +0.7 382,170 - -1.0 382,176 + +0.6 382,176 + -1.2 382,176 + -0.2 382,176 + -0.5 382,177 - +0.3 382,177 - +0.2 382,265 + +0.7 382,265 + +2.1 382,266 - -2.6 382,266 - -3.1 382,266 - -2.6 382,266 - -3.2 382,266 - -2.5 382,434 + BPHYT_RS01685 0.22 -2.2 382,475 + BPHYT_RS01685 0.27 -3.3 382,475 + BPHYT_RS01685 0.27 -3.5 382,475 + BPHYT_RS01685 0.27 -2.2 382,476 - BPHYT_RS01685 0.27 -2.1 382,476 - BPHYT_RS01685 0.27 -3.7 382,476 - BPHYT_RS01685 0.27 -2.0 382,500 + BPHYT_RS01685 0.30 -0.7 382,500 + BPHYT_RS01685 0.30 -3.1 382,500 + BPHYT_RS01685 0.30 -1.0 382,566 + BPHYT_RS01685 0.39 -2.3 382,567 - BPHYT_RS01685 0.39 -1.5 382,567 - BPHYT_RS01685 0.39 -3.1 382,567 - BPHYT_RS01685 0.39 -3.0 382,567 - BPHYT_RS01685 0.39 -1.7 382,605 - BPHYT_RS01685 0.44 -3.3 382,633 - BPHYT_RS01685 0.48 -2.5 382,957 - -2.0 382,957 - -0.5 382,957 - -2.1 382,957 - -2.5 382,957 - -2.3 382,963 - -4.2 382,963 - -2.4 383,222 - BPHYT_RS01690 0.49 -0.7 383,274 + BPHYT_RS01690 0.74 -1.6 383,328 + +0.4 383,328 + +0.2 383,329 - -0.3 383,329 - +0.1 383,329 - +0.5 383,431 + +0.1 383,432 - -0.7 383,432 - +0.6 383,537 + -0.6 383,538 - -0.9 383,569 + -0.4 383,585 - -0.2 383,709 + BPHYT_RS01695 0.16 -0.5 383,737 + BPHYT_RS01695 0.19 -0.5 383,737 + BPHYT_RS01695 0.19 +0.1 383,935 + BPHYT_RS01695 0.38 -0.2 383,950 + BPHYT_RS01695 0.39 +1.8 383,951 - BPHYT_RS01695 0.39 -0.7
Or see this region's nucleotide sequence