Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01655 and BPHYT_RS01660 are separated by 44 nucleotides BPHYT_RS01660 and BPHYT_RS01665 are separated by 57 nucleotides BPHYT_RS01665 and BPHYT_RS01670 are separated by 94 nucleotides
BPHYT_RS01655: BPHYT_RS01655 - tyrosine recombinase XerC, at 376,167 to 377,090
_RS01655
BPHYT_RS01660: BPHYT_RS01660 - hypothetical protein, at 377,135 to 377,857
_RS01660
BPHYT_RS01665: BPHYT_RS01665 - diaminopimelate epimerase, at 377,915 to 378,814
_RS01665
BPHYT_RS01670: BPHYT_RS01670 - lauroyl acyltransferase, at 378,909 to 379,793
_RS01670
Position (kb)
377
378
379 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 377.021 kb on + strand at 377.021 kb on + strand at 377.427 kb on + strand, within BPHYT_RS01660 at 377.833 kb on - strand at 377.917 kb on - strand at 378.820 kb on + strand at 378.820 kb on + strand at 378.825 kb on + strand at 378.825 kb on + strand at 378.826 kb on - strand at 378.826 kb on - strand at 378.826 kb on - strand at 378.863 kb on - strand at 378.863 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 377,021 + -3.6 377,021 + -1.1 377,427 + BPHYT_RS01660 0.40 -1.7 377,833 - -0.7 377,917 - +0.4 378,820 + -3.2 378,820 + -2.6 378,825 + -0.2 378,825 + -0.3 378,826 - -0.4 378,826 - +0.0 378,826 - +1.1 378,863 - -0.5 378,863 - +0.7
Or see this region's nucleotide sequence