Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01595 and BPHYT_RS01600 are separated by 323 nucleotides BPHYT_RS01600 and BPHYT_RS01605 overlap by 34 nucleotides BPHYT_RS01605 and BPHYT_RS01610 are separated by 227 nucleotides
BPHYT_RS01595: BPHYT_RS01595 - homoserine O-acetyltransferase, at 361,639 to 362,784
_RS01595
BPHYT_RS01600: BPHYT_RS01600 - dihydroorotate oxidase, at 363,108 to 363,791
_RS01600
BPHYT_RS01605: BPHYT_RS01605 - HAD family hydrolase, at 363,758 to 364,561
_RS01605
BPHYT_RS01610: BPHYT_RS01610 - acetylglutamate kinase, at 364,789 to 365,688
_RS01610
Position (kb) 363
364
365 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2 at 362.799 kb on - strand at 362.882 kb on + strand at 362.883 kb on - strand at 362.884 kb on + strand at 362.884 kb on + strand at 362.884 kb on + strand at 362.885 kb on - strand at 362.950 kb on + strand at 363.043 kb on - strand at 363.043 kb on - strand at 363.109 kb on + strand at 363.178 kb on + strand, within BPHYT_RS01600 at 363.178 kb on + strand, within BPHYT_RS01600 at 363.179 kb on - strand, within BPHYT_RS01600 at 363.179 kb on - strand, within BPHYT_RS01600 at 363.200 kb on + strand, within BPHYT_RS01600 at 363.200 kb on + strand, within BPHYT_RS01600 at 363.200 kb on + strand, within BPHYT_RS01600 at 363.201 kb on - strand, within BPHYT_RS01600 at 363.201 kb on - strand, within BPHYT_RS01600 at 363.202 kb on + strand, within BPHYT_RS01600 at 363.202 kb on + strand, within BPHYT_RS01600 at 363.203 kb on - strand, within BPHYT_RS01600 at 363.759 kb on + strand at 363.759 kb on + strand at 363.845 kb on + strand, within BPHYT_RS01605 at 363.872 kb on - strand, within BPHYT_RS01605 at 363.879 kb on + strand, within BPHYT_RS01605 at 363.879 kb on + strand, within BPHYT_RS01605 at 363.879 kb on + strand, within BPHYT_RS01605 at 363.879 kb on + strand, within BPHYT_RS01605 at 363.879 kb on + strand, within BPHYT_RS01605 at 363.880 kb on - strand, within BPHYT_RS01605 at 363.880 kb on - strand, within BPHYT_RS01605 at 364.111 kb on + strand, within BPHYT_RS01605 at 364.112 kb on - strand, within BPHYT_RS01605 at 364.179 kb on + strand, within BPHYT_RS01605 at 364.180 kb on - strand, within BPHYT_RS01605 at 364.180 kb on - strand, within BPHYT_RS01605 at 364.356 kb on + strand, within BPHYT_RS01605 at 364.356 kb on + strand, within BPHYT_RS01605 at 364.426 kb on - strand, within BPHYT_RS01605 at 364.426 kb on - strand, within BPHYT_RS01605 at 364.710 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 362,799 - -1.0 362,882 + -3.3 362,883 - +0.9 362,884 + -3.7 362,884 + -1.3 362,884 + -2.0 362,885 - +0.5 362,950 + -6.1 363,043 - +0.1 363,043 - -2.0 363,109 + -0.9 363,178 + BPHYT_RS01600 0.10 +0.5 363,178 + BPHYT_RS01600 0.10 +0.3 363,179 - BPHYT_RS01600 0.10 -0.7 363,179 - BPHYT_RS01600 0.10 +0.3 363,200 + BPHYT_RS01600 0.13 +0.2 363,200 + BPHYT_RS01600 0.13 +0.6 363,200 + BPHYT_RS01600 0.13 -0.4 363,201 - BPHYT_RS01600 0.14 +1.3 363,201 - BPHYT_RS01600 0.14 +0.0 363,202 + BPHYT_RS01600 0.14 +2.1 363,202 + BPHYT_RS01600 0.14 -0.7 363,203 - BPHYT_RS01600 0.14 -0.1 363,759 + -0.0 363,759 + -0.6 363,845 + BPHYT_RS01605 0.11 +0.1 363,872 - BPHYT_RS01605 0.14 +0.5 363,879 + BPHYT_RS01605 0.15 -0.6 363,879 + BPHYT_RS01605 0.15 +0.2 363,879 + BPHYT_RS01605 0.15 +0.4 363,879 + BPHYT_RS01605 0.15 -0.6 363,879 + BPHYT_RS01605 0.15 +0.3 363,880 - BPHYT_RS01605 0.15 +1.4 363,880 - BPHYT_RS01605 0.15 -1.0 364,111 + BPHYT_RS01605 0.44 -0.5 364,112 - BPHYT_RS01605 0.44 -1.4 364,179 + BPHYT_RS01605 0.52 -0.3 364,180 - BPHYT_RS01605 0.52 -0.0 364,180 - BPHYT_RS01605 0.52 +2.5 364,356 + BPHYT_RS01605 0.74 +1.8 364,356 + BPHYT_RS01605 0.74 -0.5 364,426 - BPHYT_RS01605 0.83 +0.9 364,426 - BPHYT_RS01605 0.83 +0.8 364,710 + -0.4
Or see this region's nucleotide sequence