Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01340 and BPHYT_RS01345 are separated by 17 nucleotides BPHYT_RS01345 and BPHYT_RS01350 are separated by 107 nucleotides BPHYT_RS01350 and BPHYT_RS01355 are separated by 7 nucleotides
BPHYT_RS01340: BPHYT_RS01340 - ChrB domain-containing protein, at 306,866 to 307,429
_RS01340
BPHYT_RS01345: BPHYT_RS01345 - endonuclease/exonuclease/phosphatase, at 307,447 to 308,253
_RS01345
BPHYT_RS01350: BPHYT_RS01350 - MFS transporter permease, at 308,361 to 309,623
_RS01350
BPHYT_RS01355: BPHYT_RS01355 - hypothetical protein, at 309,631 to 309,993
_RS01355
Position (kb) 308
309
310 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 307.367 kb on + strand, within BPHYT_RS01340 at 307.367 kb on + strand, within BPHYT_RS01340 at 307.427 kb on + strand at 307.427 kb on + strand at 307.467 kb on + strand at 307.468 kb on - strand at 307.471 kb on + strand at 307.471 kb on + strand at 307.471 kb on + strand at 307.472 kb on - strand at 307.472 kb on - strand at 307.472 kb on - strand at 307.978 kb on + strand, within BPHYT_RS01345 at 307.978 kb on + strand, within BPHYT_RS01345 at 307.979 kb on - strand, within BPHYT_RS01345 at 308.137 kb on + strand, within BPHYT_RS01345 at 308.225 kb on - strand at 308.251 kb on + strand at 308.252 kb on - strand at 308.252 kb on - strand at 308.296 kb on + strand at 308.296 kb on + strand at 308.296 kb on + strand at 308.297 kb on - strand at 308.301 kb on + strand at 308.301 kb on + strand at 308.301 kb on + strand at 308.301 kb on + strand at 308.302 kb on - strand at 308.476 kb on - strand at 308.493 kb on - strand, within BPHYT_RS01350 at 308.493 kb on - strand, within BPHYT_RS01350 at 308.514 kb on + strand, within BPHYT_RS01350 at 308.515 kb on + strand, within BPHYT_RS01350 at 308.515 kb on + strand, within BPHYT_RS01350 at 308.598 kb on + strand, within BPHYT_RS01350 at 308.647 kb on - strand, within BPHYT_RS01350 at 309.066 kb on + strand, within BPHYT_RS01350 at 309.192 kb on + strand, within BPHYT_RS01350 at 309.192 kb on + strand, within BPHYT_RS01350 at 309.192 kb on + strand, within BPHYT_RS01350 at 309.193 kb on - strand, within BPHYT_RS01350 at 309.193 kb on - strand, within BPHYT_RS01350 at 309.193 kb on - strand, within BPHYT_RS01350 at 309.193 kb on - strand, within BPHYT_RS01350 at 309.228 kb on + strand, within BPHYT_RS01350 at 309.366 kb on - strand, within BPHYT_RS01350 at 309.366 kb on - strand, within BPHYT_RS01350 at 309.387 kb on + strand, within BPHYT_RS01350 at 309.387 kb on + strand, within BPHYT_RS01350 at 309.387 kb on + strand, within BPHYT_RS01350 at 309.447 kb on - strand, within BPHYT_RS01350 at 309.462 kb on + strand, within BPHYT_RS01350 at 309.462 kb on + strand, within BPHYT_RS01350 at 309.462 kb on + strand, within BPHYT_RS01350 at 309.462 kb on + strand, within BPHYT_RS01350 at 309.463 kb on - strand, within BPHYT_RS01350 at 309.463 kb on - strand, within BPHYT_RS01350 at 309.463 kb on - strand, within BPHYT_RS01350 at 309.489 kb on - strand, within BPHYT_RS01350 at 309.696 kb on + strand, within BPHYT_RS01355 at 309.697 kb on - strand, within BPHYT_RS01355 at 309.700 kb on + strand, within BPHYT_RS01355 at 309.700 kb on + strand, within BPHYT_RS01355 at 309.700 kb on + strand, within BPHYT_RS01355 at 309.701 kb on - strand, within BPHYT_RS01355 at 309.701 kb on - strand, within BPHYT_RS01355 at 309.716 kb on + strand, within BPHYT_RS01355 at 309.779 kb on - strand, within BPHYT_RS01355 at 309.826 kb on + strand, within BPHYT_RS01355 at 309.827 kb on - strand, within BPHYT_RS01355 at 309.827 kb on - strand, within BPHYT_RS01355 at 309.831 kb on - strand, within BPHYT_RS01355 at 309.831 kb on - strand, within BPHYT_RS01355 at 309.842 kb on - strand, within BPHYT_RS01355 at 309.842 kb on - strand, within BPHYT_RS01355 at 309.853 kb on + strand, within BPHYT_RS01355 at 309.965 kb on + strand at 309.965 kb on + strand at 309.965 kb on + strand at 309.991 kb on + strand at 310.045 kb on + strand at 310.045 kb on + strand at 310.046 kb on - strand at 310.046 kb on - strand at 310.080 kb on + strand at 310.155 kb on + strand at 310.155 kb on + strand at 310.156 kb on - strand at 310.156 kb on - strand at 310.156 kb on - strand at 310.306 kb on + strand at 310.353 kb on + strand at 310.353 kb on + strand at 310.353 kb on + strand at 310.374 kb on - strand at 310.374 kb on - strand at 310.400 kb on - strand at 310.435 kb on - strand at 310.465 kb on - strand at 310.465 kb on - strand at 310.525 kb on + strand at 310.525 kb on + strand at 310.526 kb on - strand at 310.526 kb on - strand at 310.526 kb on - strand at 310.529 kb on + strand at 310.530 kb on - strand at 310.530 kb on - strand at 310.530 kb on - strand at 310.554 kb on - strand at 310.560 kb on + strand at 310.560 kb on + strand at 310.561 kb on - strand at 310.561 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 307,367 + BPHYT_RS01340 0.89 +0.4 307,367 + BPHYT_RS01340 0.89 -0.9 307,427 + -3.3 307,427 + -0.5 307,467 + +0.4 307,468 - -0.2 307,471 + -0.2 307,471 + -0.0 307,471 + +1.6 307,472 - +0.3 307,472 - -0.4 307,472 - +0.7 307,978 + BPHYT_RS01345 0.66 -0.4 307,978 + BPHYT_RS01345 0.66 +0.2 307,979 - BPHYT_RS01345 0.66 -0.5 308,137 + BPHYT_RS01345 0.86 -0.3 308,225 - -0.8 308,251 + -0.3 308,252 - -0.2 308,252 - -1.2 308,296 + -0.3 308,296 + -0.9 308,296 + +0.1 308,297 - +0.2 308,301 + +0.1 308,301 + +4.9 308,301 + -2.3 308,301 + +1.1 308,302 - +0.3 308,476 - +2.8 308,493 - BPHYT_RS01350 0.10 -0.2 308,493 - BPHYT_RS01350 0.10 -0.2 308,514 + BPHYT_RS01350 0.12 +0.7 308,515 + BPHYT_RS01350 0.12 -1.5 308,515 + BPHYT_RS01350 0.12 +0.1 308,598 + BPHYT_RS01350 0.19 -0.3 308,647 - BPHYT_RS01350 0.23 -0.3 309,066 + BPHYT_RS01350 0.56 +0.5 309,192 + BPHYT_RS01350 0.66 -0.7 309,192 + BPHYT_RS01350 0.66 +3.1 309,192 + BPHYT_RS01350 0.66 -0.5 309,193 - BPHYT_RS01350 0.66 +0.5 309,193 - BPHYT_RS01350 0.66 -0.1 309,193 - BPHYT_RS01350 0.66 -0.4 309,193 - BPHYT_RS01350 0.66 +2.4 309,228 + BPHYT_RS01350 0.69 -0.1 309,366 - BPHYT_RS01350 0.80 +1.0 309,366 - BPHYT_RS01350 0.80 +0.5 309,387 + BPHYT_RS01350 0.81 +0.2 309,387 + BPHYT_RS01350 0.81 +0.2 309,387 + BPHYT_RS01350 0.81 +1.0 309,447 - BPHYT_RS01350 0.86 +1.3 309,462 + BPHYT_RS01350 0.87 -0.9 309,462 + BPHYT_RS01350 0.87 -0.1 309,462 + BPHYT_RS01350 0.87 -0.4 309,462 + BPHYT_RS01350 0.87 +0.4 309,463 - BPHYT_RS01350 0.87 +0.9 309,463 - BPHYT_RS01350 0.87 -2.0 309,463 - BPHYT_RS01350 0.87 +0.4 309,489 - BPHYT_RS01350 0.89 -0.2 309,696 + BPHYT_RS01355 0.18 +0.1 309,697 - BPHYT_RS01355 0.18 -3.3 309,700 + BPHYT_RS01355 0.19 +0.1 309,700 + BPHYT_RS01355 0.19 +0.5 309,700 + BPHYT_RS01355 0.19 +0.7 309,701 - BPHYT_RS01355 0.19 +0.1 309,701 - BPHYT_RS01355 0.19 -0.4 309,716 + BPHYT_RS01355 0.23 -0.5 309,779 - BPHYT_RS01355 0.41 -0.7 309,826 + BPHYT_RS01355 0.54 +1.0 309,827 - BPHYT_RS01355 0.54 -0.9 309,827 - BPHYT_RS01355 0.54 +0.2 309,831 - BPHYT_RS01355 0.55 +0.2 309,831 - BPHYT_RS01355 0.55 +0.0 309,842 - BPHYT_RS01355 0.58 +0.3 309,842 - BPHYT_RS01355 0.58 -1.1 309,853 + BPHYT_RS01355 0.61 -1.6 309,965 + -0.3 309,965 + -1.7 309,965 + -0.5 309,991 + -1.4 310,045 + +0.1 310,045 + -0.1 310,046 - -0.9 310,046 - +0.0 310,080 + -0.8 310,155 + -0.6 310,155 + -0.1 310,156 - -0.9 310,156 - +0.5 310,156 - +0.4 310,306 + +0.5 310,353 + +1.8 310,353 + -1.3 310,353 + -2.8 310,374 - -2.0 310,374 - -1.1 310,400 - -0.7 310,435 - -0.7 310,465 - +1.0 310,465 - -3.8 310,525 + -0.6 310,525 + +0.8 310,526 - +0.1 310,526 - +0.2 310,526 - +0.2 310,529 + -0.9 310,530 - +0.2 310,530 - +0.1 310,530 - -0.2 310,554 - -0.1 310,560 + +0.8 310,560 + +0.4 310,561 - +0.4 310,561 - -2.6
Or see this region's nucleotide sequence