Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01295 and BPHYT_RS01300 are separated by 87 nucleotides BPHYT_RS01300 and BPHYT_RS01305 are separated by 116 nucleotides BPHYT_RS01305 and BPHYT_RS01310 are separated by 115 nucleotides
BPHYT_RS01295: BPHYT_RS01295 - glutathione S-transferase, at 296,422 to 297,096
_RS01295
BPHYT_RS01300: BPHYT_RS01300 - ArsR family transcriptional regulator, at 297,184 to 297,879
_RS01300
BPHYT_RS01305: BPHYT_RS01305 - MFS transporter, at 297,996 to 299,387
_RS01305
BPHYT_RS01310: BPHYT_RS01310 - L-lysine 6-monooxygenase, at 299,503 to 300,828
_RS01310
Position (kb) 297
298
299
300 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 297.059 kb on + strand at 297.094 kb on + strand at 297.193 kb on - strand at 297.196 kb on - strand at 297.339 kb on - strand, within BPHYT_RS01300 at 297.542 kb on + strand, within BPHYT_RS01300 at 297.672 kb on - strand, within BPHYT_RS01300 at 297.672 kb on - strand, within BPHYT_RS01300 at 297.811 kb on - strand at 297.864 kb on + strand at 297.864 kb on + strand at 297.864 kb on + strand at 297.865 kb on - strand at 297.897 kb on + strand at 297.907 kb on + strand at 297.976 kb on - strand at 297.993 kb on + strand at 298.045 kb on + strand at 298.082 kb on - strand at 298.085 kb on + strand at 298.085 kb on + strand at 298.085 kb on + strand at 298.086 kb on - strand at 298.086 kb on - strand at 298.139 kb on + strand, within BPHYT_RS01305 at 298.171 kb on - strand, within BPHYT_RS01305 at 298.173 kb on - strand, within BPHYT_RS01305 at 298.173 kb on - strand, within BPHYT_RS01305 at 298.382 kb on + strand, within BPHYT_RS01305 at 298.382 kb on + strand, within BPHYT_RS01305 at 298.382 kb on + strand, within BPHYT_RS01305 at 298.382 kb on + strand, within BPHYT_RS01305 at 298.538 kb on - strand, within BPHYT_RS01305 at 298.875 kb on + strand, within BPHYT_RS01305 at 298.986 kb on + strand, within BPHYT_RS01305 at 299.025 kb on + strand, within BPHYT_RS01305 at 299.073 kb on + strand, within BPHYT_RS01305 at 299.126 kb on + strand, within BPHYT_RS01305 at 299.336 kb on - strand at 299.353 kb on + strand at 299.497 kb on + strand at 299.498 kb on - strand at 299.511 kb on + strand at 299.511 kb on + strand at 299.549 kb on + strand at 299.683 kb on + strand, within BPHYT_RS01310 at 299.719 kb on - strand, within BPHYT_RS01310 at 299.719 kb on - strand, within BPHYT_RS01310 at 299.796 kb on - strand, within BPHYT_RS01310 at 299.818 kb on + strand, within BPHYT_RS01310 at 299.818 kb on + strand, within BPHYT_RS01310 at 299.819 kb on - strand, within BPHYT_RS01310 at 300.011 kb on + strand, within BPHYT_RS01310 at 300.025 kb on + strand, within BPHYT_RS01310 at 300.026 kb on - strand, within BPHYT_RS01310 at 300.209 kb on - strand, within BPHYT_RS01310 at 300.209 kb on - strand, within BPHYT_RS01310 at 300.211 kb on - strand, within BPHYT_RS01310 at 300.211 kb on - strand, within BPHYT_RS01310 at 300.211 kb on - strand, within BPHYT_RS01310 at 300.221 kb on - strand, within BPHYT_RS01310 at 300.221 kb on - strand, within BPHYT_RS01310 at 300.229 kb on + strand, within BPHYT_RS01310 at 300.230 kb on - strand, within BPHYT_RS01310 at 300.230 kb on - strand, within BPHYT_RS01310 at 300.321 kb on + strand, within BPHYT_RS01310 at 300.325 kb on + strand, within BPHYT_RS01310
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 297,059 + +0.1 297,094 + -0.3 297,193 - -2.9 297,196 - +0.1 297,339 - BPHYT_RS01300 0.22 +0.8 297,542 + BPHYT_RS01300 0.51 -0.6 297,672 - BPHYT_RS01300 0.70 -0.3 297,672 - BPHYT_RS01300 0.70 -0.2 297,811 - +0.1 297,864 + +0.4 297,864 + -0.6 297,864 + -0.5 297,865 - -1.2 297,897 + +0.1 297,907 + +2.7 297,976 - +2.6 297,993 + -0.5 298,045 + -1.5 298,082 - -0.3 298,085 + -2.1 298,085 + -0.9 298,085 + -2.8 298,086 - -0.9 298,086 - -0.8 298,139 + BPHYT_RS01305 0.10 -0.9 298,171 - BPHYT_RS01305 0.13 +0.9 298,173 - BPHYT_RS01305 0.13 -0.5 298,173 - BPHYT_RS01305 0.13 -1.9 298,382 + BPHYT_RS01305 0.28 +1.3 298,382 + BPHYT_RS01305 0.28 -1.2 298,382 + BPHYT_RS01305 0.28 -1.1 298,382 + BPHYT_RS01305 0.28 -1.0 298,538 - BPHYT_RS01305 0.39 -1.8 298,875 + BPHYT_RS01305 0.63 -0.5 298,986 + BPHYT_RS01305 0.71 -1.3 299,025 + BPHYT_RS01305 0.74 +0.0 299,073 + BPHYT_RS01305 0.77 -0.3 299,126 + BPHYT_RS01305 0.81 -0.7 299,336 - -1.2 299,353 + +0.0 299,497 + -0.9 299,498 - -0.5 299,511 + -0.9 299,511 + -0.5 299,549 + -0.0 299,683 + BPHYT_RS01310 0.14 +0.0 299,719 - BPHYT_RS01310 0.16 -0.5 299,719 - BPHYT_RS01310 0.16 +0.3 299,796 - BPHYT_RS01310 0.22 -0.3 299,818 + BPHYT_RS01310 0.24 +0.5 299,818 + BPHYT_RS01310 0.24 -1.5 299,819 - BPHYT_RS01310 0.24 -1.2 300,011 + BPHYT_RS01310 0.38 -0.0 300,025 + BPHYT_RS01310 0.39 -0.2 300,026 - BPHYT_RS01310 0.39 -0.4 300,209 - BPHYT_RS01310 0.53 +2.5 300,209 - BPHYT_RS01310 0.53 -0.3 300,211 - BPHYT_RS01310 0.53 -0.4 300,211 - BPHYT_RS01310 0.53 -0.1 300,211 - BPHYT_RS01310 0.53 -1.5 300,221 - BPHYT_RS01310 0.54 +0.4 300,221 - BPHYT_RS01310 0.54 -1.3 300,229 + BPHYT_RS01310 0.55 -0.5 300,230 - BPHYT_RS01310 0.55 +0.8 300,230 - BPHYT_RS01310 0.55 -0.3 300,321 + BPHYT_RS01310 0.62 +2.1 300,325 + BPHYT_RS01310 0.62 +0.4
Or see this region's nucleotide sequence