Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01025 and BPHYT_RS01030 are separated by 366 nucleotides BPHYT_RS01030 and BPHYT_RS01035 are separated by 341 nucleotides BPHYT_RS01035 and BPHYT_RS01040 overlap by 4 nucleotides
BPHYT_RS01025: BPHYT_RS01025 - hypothetical protein, at 236,189 to 236,752
_RS01025
BPHYT_RS01030: BPHYT_RS01030 - bacterioferritin, at 237,119 to 237,598
_RS01030
BPHYT_RS01035: BPHYT_RS01035 - cyanate hydratase, at 237,940 to 238,389
_RS01035
BPHYT_RS01040: BPHYT_RS01040 - Fis family transcriptional regulator, at 238,386 to 238,841
_RS01040
Position (kb)
237
238 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 236.155 kb on + strand at 236.155 kb on + strand at 236.160 kb on - strand at 236.213 kb on - strand at 237.025 kb on + strand at 237.025 kb on + strand at 237.025 kb on + strand at 237.026 kb on - strand at 237.030 kb on - strand at 237.030 kb on - strand at 237.030 kb on - strand at 237.030 kb on - strand at 237.030 kb on - strand at 237.030 kb on - strand at 237.120 kb on + strand at 237.153 kb on + strand at 237.279 kb on + strand, within BPHYT_RS01030 at 237.466 kb on - strand, within BPHYT_RS01030 at 237.504 kb on + strand, within BPHYT_RS01030 at 237.504 kb on + strand, within BPHYT_RS01030 at 237.505 kb on - strand, within BPHYT_RS01030 at 237.525 kb on + strand, within BPHYT_RS01030 at 237.525 kb on + strand, within BPHYT_RS01030 at 237.627 kb on - strand at 237.702 kb on + strand at 237.810 kb on + strand at 237.810 kb on + strand at 237.841 kb on + strand at 237.879 kb on - strand at 237.889 kb on + strand at 237.889 kb on + strand at 237.890 kb on - strand at 238.204 kb on + strand, within BPHYT_RS01035 at 238.204 kb on + strand, within BPHYT_RS01035 at 238.376 kb on - strand at 238.384 kb on + strand at 238.385 kb on - strand at 238.388 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 236,155 + +0.1 236,155 + -2.3 236,160 - -1.1 236,213 - -1.4 237,025 + -0.5 237,025 + +0.5 237,025 + -0.9 237,026 - -1.4 237,030 - -0.5 237,030 - -1.2 237,030 - +1.1 237,030 - +1.4 237,030 - +0.7 237,030 - +1.8 237,120 + -0.4 237,153 + +0.5 237,279 + BPHYT_RS01030 0.33 -0.1 237,466 - BPHYT_RS01030 0.72 -0.3 237,504 + BPHYT_RS01030 0.80 +2.1 237,504 + BPHYT_RS01030 0.80 +0.3 237,505 - BPHYT_RS01030 0.80 +0.1 237,525 + BPHYT_RS01030 0.85 -0.2 237,525 + BPHYT_RS01030 0.85 -0.1 237,627 - +0.1 237,702 + -0.6 237,810 + -0.7 237,810 + +0.3 237,841 + +0.6 237,879 - -0.3 237,889 + +0.0 237,889 + +0.2 237,890 - +0.3 238,204 + BPHYT_RS01035 0.59 -0.4 238,204 + BPHYT_RS01035 0.59 -1.3 238,376 - +0.1 238,384 + +1.2 238,385 - +0.7 238,388 - +0.2
Or see this region's nucleotide sequence