Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00735 and BPHYT_RS00740 overlap by 4 nucleotides BPHYT_RS00740 and BPHYT_RS00745 are separated by 290 nucleotides BPHYT_RS00745 and BPHYT_RS00750 overlap by 4 nucleotides BPHYT_RS00750 and BPHYT_RS00755 are separated by 526 nucleotides
BPHYT_RS00735: BPHYT_RS00735 - AlpA family transcriptional regulator, at 170,907 to 171,149
_RS00735
BPHYT_RS00740: BPHYT_RS00740 - hypothetical protein, at 171,146 to 171,472
_RS00740
BPHYT_RS00745: BPHYT_RS00745 - transcriptional regulator, at 171,763 to 172,167
_RS00745
BPHYT_RS00750: BPHYT_RS00750 - SpoVT/AbrB domain-containing protein, at 172,164 to 172,436
_RS00750
BPHYT_RS00755: BPHYT_RS00755 - cobyric acid synthase CobQ, at 172,963 to 173,835
_RS00755
Position (kb) 171
172
173 Strain fitness (log2 ratio) -1
0
1
2 at 170.764 kb on + strand at 170.765 kb on - strand at 170.925 kb on - strand at 170.925 kb on - strand at 170.991 kb on + strand, within BPHYT_RS00735 at 170.992 kb on - strand, within BPHYT_RS00735 at 170.999 kb on + strand, within BPHYT_RS00735 at 171.000 kb on - strand, within BPHYT_RS00735 at 171.000 kb on - strand, within BPHYT_RS00735 at 171.299 kb on + strand, within BPHYT_RS00740 at 171.299 kb on + strand, within BPHYT_RS00740 at 171.300 kb on - strand, within BPHYT_RS00740 at 171.300 kb on - strand, within BPHYT_RS00740 at 171.529 kb on + strand at 171.530 kb on - strand at 171.530 kb on - strand at 171.533 kb on + strand at 171.533 kb on + strand at 171.664 kb on - strand at 172.158 kb on - strand at 172.171 kb on - strand at 172.171 kb on - strand at 172.171 kb on - strand at 172.171 kb on - strand at 172.318 kb on + strand, within BPHYT_RS00750 at 172.319 kb on - strand, within BPHYT_RS00750 at 172.482 kb on + strand at 172.504 kb on + strand at 172.505 kb on - strand at 172.505 kb on - strand at 172.507 kb on + strand at 172.508 kb on - strand at 172.508 kb on - strand at 172.508 kb on - strand at 172.508 kb on - strand at 172.695 kb on + strand at 172.695 kb on + strand at 172.696 kb on - strand at 172.703 kb on + strand at 172.754 kb on - strand at 172.754 kb on - strand at 172.830 kb on + strand at 172.830 kb on + strand at 172.830 kb on + strand at 173.069 kb on + strand, within BPHYT_RS00755 at 173.069 kb on + strand, within BPHYT_RS00755 at 173.069 kb on + strand, within BPHYT_RS00755 at 173.070 kb on - strand, within BPHYT_RS00755 at 173.070 kb on - strand, within BPHYT_RS00755
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 170,764 + -0.7 170,765 - -0.3 170,925 - +1.8 170,925 - +1.5 170,991 + BPHYT_RS00735 0.35 -0.5 170,992 - BPHYT_RS00735 0.35 -0.2 170,999 + BPHYT_RS00735 0.38 +0.5 171,000 - BPHYT_RS00735 0.38 +0.7 171,000 - BPHYT_RS00735 0.38 -0.6 171,299 + BPHYT_RS00740 0.47 -0.5 171,299 + BPHYT_RS00740 0.47 -0.0 171,300 - BPHYT_RS00740 0.47 -1.4 171,300 - BPHYT_RS00740 0.47 +0.4 171,529 + -0.8 171,530 - +0.0 171,530 - -0.5 171,533 + +1.1 171,533 + -0.1 171,664 - +0.9 172,158 - -0.4 172,171 - +0.5 172,171 - -0.5 172,171 - -0.1 172,171 - +0.4 172,318 + BPHYT_RS00750 0.56 -1.0 172,319 - BPHYT_RS00750 0.57 -0.8 172,482 + +0.2 172,504 + +0.2 172,505 - -0.6 172,505 - -0.8 172,507 + -0.6 172,508 - +1.9 172,508 - -0.4 172,508 - +0.9 172,508 - +0.2 172,695 + -0.4 172,695 + -0.2 172,696 - -0.9 172,703 + +0.1 172,754 - +0.1 172,754 - +0.1 172,830 + -0.4 172,830 + -0.1 172,830 + -0.7 173,069 + BPHYT_RS00755 0.12 -0.4 173,069 + BPHYT_RS00755 0.12 -0.1 173,069 + BPHYT_RS00755 0.12 -0.5 173,070 - BPHYT_RS00755 0.12 +0.6 173,070 - BPHYT_RS00755 0.12 -0.1
Or see this region's nucleotide sequence