Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00670 and BPHYT_RS00675 are separated by 239 nucleotides BPHYT_RS00675 and BPHYT_RS00680 are separated by 54 nucleotides
BPHYT_RS00670: BPHYT_RS00670 - acyl-CoA dehydrogenase, at 157,784 to 159,094
_RS00670
BPHYT_RS00675: BPHYT_RS00675 - GntR family transcriptional regulator, at 159,334 to 160,170
_RS00675
BPHYT_RS00680: BPHYT_RS00680 - amidase, at 160,225 to 161,649
_RS00680
Position (kb) 159
160
161 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 158.415 kb on + strand, within BPHYT_RS00670 at 158.488 kb on + strand, within BPHYT_RS00670 at 158.488 kb on + strand, within BPHYT_RS00670 at 158.489 kb on - strand, within BPHYT_RS00670 at 158.520 kb on + strand, within BPHYT_RS00670 at 158.642 kb on - strand at 158.642 kb on - strand, within BPHYT_RS00670 at 159.084 kb on + strand at 159.085 kb on - strand at 159.146 kb on + strand at 159.172 kb on + strand at 159.173 kb on - strand at 159.186 kb on - strand at 159.221 kb on + strand at 159.287 kb on + strand at 159.320 kb on + strand at 159.320 kb on + strand at 159.321 kb on - strand at 159.321 kb on - strand at 159.333 kb on + strand at 159.333 kb on + strand at 159.333 kb on + strand at 159.334 kb on - strand at 159.606 kb on + strand, within BPHYT_RS00675 at 159.607 kb on - strand, within BPHYT_RS00675 at 159.649 kb on + strand, within BPHYT_RS00675 at 159.650 kb on - strand, within BPHYT_RS00675 at 159.685 kb on + strand, within BPHYT_RS00675 at 159.685 kb on + strand, within BPHYT_RS00675 at 159.685 kb on + strand, within BPHYT_RS00675 at 159.686 kb on - strand, within BPHYT_RS00675 at 159.686 kb on - strand, within BPHYT_RS00675 at 159.714 kb on - strand, within BPHYT_RS00675 at 159.783 kb on - strand, within BPHYT_RS00675 at 159.921 kb on + strand, within BPHYT_RS00675 at 159.922 kb on - strand, within BPHYT_RS00675 at 159.961 kb on + strand, within BPHYT_RS00675 at 159.962 kb on - strand, within BPHYT_RS00675 at 159.962 kb on - strand, within BPHYT_RS00675 at 160.216 kb on + strand at 160.220 kb on + strand at 160.220 kb on + strand at 160.221 kb on - strand at 160.221 kb on - strand at 160.221 kb on - strand at 160.242 kb on + strand at 160.401 kb on - strand, within BPHYT_RS00680 at 160.405 kb on - strand, within BPHYT_RS00680 at 160.405 kb on - strand, within BPHYT_RS00680 at 160.405 kb on - strand, within BPHYT_RS00680 at 160.505 kb on + strand, within BPHYT_RS00680 at 160.506 kb on - strand, within BPHYT_RS00680 at 160.506 kb on - strand, within BPHYT_RS00680 at 160.506 kb on - strand, within BPHYT_RS00680 at 160.506 kb on - strand, within BPHYT_RS00680 at 160.628 kb on + strand, within BPHYT_RS00680 at 160.628 kb on + strand, within BPHYT_RS00680 at 160.629 kb on - strand, within BPHYT_RS00680 at 160.629 kb on - strand, within BPHYT_RS00680 at 160.681 kb on - strand, within BPHYT_RS00680 at 160.856 kb on + strand, within BPHYT_RS00680 at 160.856 kb on + strand, within BPHYT_RS00680 at 160.856 kb on + strand, within BPHYT_RS00680 at 160.857 kb on - strand, within BPHYT_RS00680 at 160.857 kb on - strand, within BPHYT_RS00680 at 160.857 kb on - strand, within BPHYT_RS00680 at 160.862 kb on - strand, within BPHYT_RS00680 at 160.907 kb on + strand, within BPHYT_RS00680 at 160.934 kb on + strand, within BPHYT_RS00680 at 161.013 kb on + strand, within BPHYT_RS00680 at 161.085 kb on + strand, within BPHYT_RS00680 at 161.151 kb on + strand, within BPHYT_RS00680 at 161.151 kb on + strand, within BPHYT_RS00680
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 158,415 + BPHYT_RS00670 0.48 +0.1 158,488 + BPHYT_RS00670 0.54 -0.6 158,488 + BPHYT_RS00670 0.54 -0.5 158,489 - BPHYT_RS00670 0.54 -0.1 158,520 + BPHYT_RS00670 0.56 -0.0 158,642 - +0.1 158,642 - BPHYT_RS00670 0.65 +0.8 159,084 + -0.5 159,085 - -0.9 159,146 + -3.2 159,172 + +0.1 159,173 - -0.5 159,186 - -0.2 159,221 + -1.0 159,287 + -0.3 159,320 + +0.7 159,320 + -0.5 159,321 - -0.7 159,321 - +0.1 159,333 + -1.0 159,333 + -0.2 159,333 + +1.3 159,334 - -1.3 159,606 + BPHYT_RS00675 0.32 +0.1 159,607 - BPHYT_RS00675 0.33 -1.8 159,649 + BPHYT_RS00675 0.38 -0.6 159,650 - BPHYT_RS00675 0.38 -2.3 159,685 + BPHYT_RS00675 0.42 -0.8 159,685 + BPHYT_RS00675 0.42 -0.2 159,685 + BPHYT_RS00675 0.42 -3.2 159,686 - BPHYT_RS00675 0.42 +1.1 159,686 - BPHYT_RS00675 0.42 -1.4 159,714 - BPHYT_RS00675 0.45 +1.2 159,783 - BPHYT_RS00675 0.54 -0.6 159,921 + BPHYT_RS00675 0.70 -1.1 159,922 - BPHYT_RS00675 0.70 -1.9 159,961 + BPHYT_RS00675 0.75 -1.2 159,962 - BPHYT_RS00675 0.75 -1.2 159,962 - BPHYT_RS00675 0.75 -0.1 160,216 + -0.6 160,220 + +0.4 160,220 + -0.8 160,221 - -0.2 160,221 - -1.1 160,221 - -0.2 160,242 + +0.0 160,401 - BPHYT_RS00680 0.12 -3.2 160,405 - BPHYT_RS00680 0.13 -1.6 160,405 - BPHYT_RS00680 0.13 +0.0 160,405 - BPHYT_RS00680 0.13 -1.0 160,505 + BPHYT_RS00680 0.20 +0.1 160,506 - BPHYT_RS00680 0.20 +0.1 160,506 - BPHYT_RS00680 0.20 +1.0 160,506 - BPHYT_RS00680 0.20 -0.1 160,506 - BPHYT_RS00680 0.20 -0.5 160,628 + BPHYT_RS00680 0.28 +0.9 160,628 + BPHYT_RS00680 0.28 +1.7 160,629 - BPHYT_RS00680 0.28 -0.5 160,629 - BPHYT_RS00680 0.28 -0.3 160,681 - BPHYT_RS00680 0.32 +1.1 160,856 + BPHYT_RS00680 0.44 +0.1 160,856 + BPHYT_RS00680 0.44 +0.9 160,856 + BPHYT_RS00680 0.44 -1.0 160,857 - BPHYT_RS00680 0.44 +0.0 160,857 - BPHYT_RS00680 0.44 +0.3 160,857 - BPHYT_RS00680 0.44 -0.7 160,862 - BPHYT_RS00680 0.45 -1.0 160,907 + BPHYT_RS00680 0.48 +1.1 160,934 + BPHYT_RS00680 0.50 +0.8 161,013 + BPHYT_RS00680 0.55 -0.2 161,085 + BPHYT_RS00680 0.60 -0.6 161,151 + BPHYT_RS00680 0.65 -0.5 161,151 + BPHYT_RS00680 0.65 -0.3
Or see this region's nucleotide sequence