Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00660 and BPHYT_RS00665 overlap by 17 nucleotides BPHYT_RS00665 and BPHYT_RS00670 overlap by 11 nucleotides BPHYT_RS00670 and BPHYT_RS00675 are separated by 239 nucleotides
BPHYT_RS00660: BPHYT_RS00660 - 3-oxoacyl-ACP synthase, at 156,510 to 157,532
_RS00660
BPHYT_RS00665: BPHYT_RS00665 - phosphopantetheine-binding protein, at 157,516 to 157,794
_RS00665
BPHYT_RS00670: BPHYT_RS00670 - acyl-CoA dehydrogenase, at 157,784 to 159,094
_RS00670
BPHYT_RS00675: BPHYT_RS00675 - GntR family transcriptional regulator, at 159,334 to 160,170
_RS00675
Position (kb) 157
158
159
160 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 156.869 kb on + strand, within BPHYT_RS00660 at 157.152 kb on + strand, within BPHYT_RS00660 at 157.298 kb on - strand, within BPHYT_RS00660 at 157.384 kb on + strand, within BPHYT_RS00660 at 157.385 kb on - strand, within BPHYT_RS00660 at 157.437 kb on + strand at 157.474 kb on + strand at 157.820 kb on + strand at 157.820 kb on + strand at 157.821 kb on - strand at 157.821 kb on - strand at 158.090 kb on - strand, within BPHYT_RS00670 at 158.319 kb on + strand, within BPHYT_RS00670 at 158.320 kb on - strand, within BPHYT_RS00670 at 158.415 kb on + strand, within BPHYT_RS00670 at 158.488 kb on + strand, within BPHYT_RS00670 at 158.488 kb on + strand, within BPHYT_RS00670 at 158.489 kb on - strand, within BPHYT_RS00670 at 158.520 kb on + strand, within BPHYT_RS00670 at 158.642 kb on - strand at 158.642 kb on - strand, within BPHYT_RS00670 at 159.084 kb on + strand at 159.085 kb on - strand at 159.146 kb on + strand at 159.172 kb on + strand at 159.173 kb on - strand at 159.186 kb on - strand at 159.221 kb on + strand at 159.287 kb on + strand at 159.320 kb on + strand at 159.320 kb on + strand at 159.321 kb on - strand at 159.321 kb on - strand at 159.333 kb on + strand at 159.333 kb on + strand at 159.333 kb on + strand at 159.334 kb on - strand at 159.606 kb on + strand, within BPHYT_RS00675 at 159.607 kb on - strand, within BPHYT_RS00675 at 159.649 kb on + strand, within BPHYT_RS00675 at 159.650 kb on - strand, within BPHYT_RS00675 at 159.685 kb on + strand, within BPHYT_RS00675 at 159.685 kb on + strand, within BPHYT_RS00675 at 159.685 kb on + strand, within BPHYT_RS00675 at 159.686 kb on - strand, within BPHYT_RS00675 at 159.686 kb on - strand, within BPHYT_RS00675 at 159.714 kb on - strand, within BPHYT_RS00675 at 159.783 kb on - strand, within BPHYT_RS00675 at 159.921 kb on + strand, within BPHYT_RS00675 at 159.922 kb on - strand, within BPHYT_RS00675 at 159.961 kb on + strand, within BPHYT_RS00675 at 159.962 kb on - strand, within BPHYT_RS00675 at 159.962 kb on - strand, within BPHYT_RS00675
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 156,869 + BPHYT_RS00660 0.35 +0.0 157,152 + BPHYT_RS00660 0.63 -1.5 157,298 - BPHYT_RS00660 0.77 -0.0 157,384 + BPHYT_RS00660 0.85 -0.1 157,385 - BPHYT_RS00660 0.86 -1.8 157,437 + +0.2 157,474 + -0.2 157,820 + +0.7 157,820 + -0.9 157,821 - -0.9 157,821 - +0.9 158,090 - BPHYT_RS00670 0.23 -0.5 158,319 + BPHYT_RS00670 0.41 -0.3 158,320 - BPHYT_RS00670 0.41 -0.4 158,415 + BPHYT_RS00670 0.48 +0.1 158,488 + BPHYT_RS00670 0.54 -0.6 158,488 + BPHYT_RS00670 0.54 -0.5 158,489 - BPHYT_RS00670 0.54 -0.1 158,520 + BPHYT_RS00670 0.56 -0.0 158,642 - +0.1 158,642 - BPHYT_RS00670 0.65 +0.8 159,084 + -0.5 159,085 - -0.9 159,146 + -3.2 159,172 + +0.1 159,173 - -0.5 159,186 - -0.2 159,221 + -1.0 159,287 + -0.3 159,320 + +0.7 159,320 + -0.5 159,321 - -0.7 159,321 - +0.1 159,333 + -1.0 159,333 + -0.2 159,333 + +1.3 159,334 - -1.3 159,606 + BPHYT_RS00675 0.32 +0.1 159,607 - BPHYT_RS00675 0.33 -1.8 159,649 + BPHYT_RS00675 0.38 -0.6 159,650 - BPHYT_RS00675 0.38 -2.3 159,685 + BPHYT_RS00675 0.42 -0.8 159,685 + BPHYT_RS00675 0.42 -0.2 159,685 + BPHYT_RS00675 0.42 -3.2 159,686 - BPHYT_RS00675 0.42 +1.1 159,686 - BPHYT_RS00675 0.42 -1.4 159,714 - BPHYT_RS00675 0.45 +1.2 159,783 - BPHYT_RS00675 0.54 -0.6 159,921 + BPHYT_RS00675 0.70 -1.1 159,922 - BPHYT_RS00675 0.70 -1.9 159,961 + BPHYT_RS00675 0.75 -1.2 159,962 - BPHYT_RS00675 0.75 -1.2 159,962 - BPHYT_RS00675 0.75 -0.1
Or see this region's nucleotide sequence