Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00265 and BPHYT_RS00270 overlap by 4 nucleotides BPHYT_RS00270 and BPHYT_RS00275 are separated by 37 nucleotides BPHYT_RS00275 and BPHYT_RS00280 are separated by 2 nucleotides
BPHYT_RS00265: BPHYT_RS00265 - branched-chain amino acid ABC transporter substrate-binding protein, at 67,674 to 69,488
_RS00265
BPHYT_RS00270: BPHYT_RS00270 - branched-chain amino acid ABC transporter, at 69,485 to 70,186
_RS00270
BPHYT_RS00275: BPHYT_RS00275 - urease subunit beta, at 70,224 to 70,910
_RS00275
BPHYT_RS00280: BPHYT_RS00280 - urease subunit alpha, at 70,913 to 72,628
_RS00280
Position (kb)
70
71 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 69.286 kb on + strand, within BPHYT_RS00265 at 69.315 kb on - strand at 69.315 kb on - strand at 69.453 kb on + strand at 69.453 kb on + strand at 69.454 kb on - strand at 69.455 kb on + strand at 69.456 kb on - strand at 69.525 kb on - strand at 69.732 kb on - strand, within BPHYT_RS00270 at 69.732 kb on - strand, within BPHYT_RS00270 at 70.094 kb on + strand, within BPHYT_RS00270 at 70.095 kb on - strand, within BPHYT_RS00270 at 70.306 kb on + strand, within BPHYT_RS00275 at 70.307 kb on - strand, within BPHYT_RS00275 at 70.460 kb on - strand, within BPHYT_RS00275 at 70.460 kb on - strand, within BPHYT_RS00275 at 70.460 kb on - strand, within BPHYT_RS00275 at 70.607 kb on + strand, within BPHYT_RS00275 at 70.608 kb on - strand, within BPHYT_RS00275 at 70.683 kb on - strand, within BPHYT_RS00275 at 70.772 kb on + strand, within BPHYT_RS00275 at 70.772 kb on + strand, within BPHYT_RS00275 at 70.772 kb on + strand, within BPHYT_RS00275 at 70.773 kb on - strand, within BPHYT_RS00275 at 70.908 kb on + strand at 70.908 kb on + strand at 70.909 kb on - strand at 70.909 kb on - strand at 70.909 kb on - strand at 70.909 kb on - strand at 70.941 kb on - strand at 71.200 kb on + strand, within BPHYT_RS00280 at 71.200 kb on + strand, within BPHYT_RS00280 at 71.733 kb on - strand, within BPHYT_RS00280 at 71.733 kb on - strand, within BPHYT_RS00280 at 71.842 kb on + strand, within BPHYT_RS00280 at 71.842 kb on + strand, within BPHYT_RS00280 at 71.843 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 69,286 + BPHYT_RS00265 0.89 -2.4 69,315 - -1.5 69,315 - -1.0 69,453 + -0.2 69,453 + +0.4 69,454 - -0.5 69,455 + -0.0 69,456 - -0.6 69,525 - -0.7 69,732 - BPHYT_RS00270 0.35 +1.0 69,732 - BPHYT_RS00270 0.35 +0.5 70,094 + BPHYT_RS00270 0.87 -1.1 70,095 - BPHYT_RS00270 0.87 -0.9 70,306 + BPHYT_RS00275 0.12 -0.1 70,307 - BPHYT_RS00275 0.12 -3.2 70,460 - BPHYT_RS00275 0.34 +0.3 70,460 - BPHYT_RS00275 0.34 +1.2 70,460 - BPHYT_RS00275 0.34 -0.2 70,607 + BPHYT_RS00275 0.56 -0.4 70,608 - BPHYT_RS00275 0.56 -0.1 70,683 - BPHYT_RS00275 0.67 +0.1 70,772 + BPHYT_RS00275 0.80 +0.8 70,772 + BPHYT_RS00275 0.80 -0.7 70,772 + BPHYT_RS00275 0.80 -0.3 70,773 - BPHYT_RS00275 0.80 +0.3 70,908 + -1.4 70,908 + -2.8 70,909 - +0.3 70,909 - -0.2 70,909 - -1.2 70,909 - +0.6 70,941 - -1.2 71,200 + BPHYT_RS00280 0.17 +0.9 71,200 + BPHYT_RS00280 0.17 +0.9 71,733 - BPHYT_RS00280 0.48 +1.7 71,733 - BPHYT_RS00280 0.48 -0.4 71,842 + BPHYT_RS00280 0.54 +0.0 71,842 + BPHYT_RS00280 0.54 +0.1 71,843 - -0.9
Or see this region's nucleotide sequence