Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00255 and BPHYT_RS00260 are separated by 80 nucleotides BPHYT_RS00260 and BPHYT_RS00265 overlap by 8 nucleotides BPHYT_RS00265 and BPHYT_RS00270 overlap by 4 nucleotides BPHYT_RS00270 and BPHYT_RS00275 are separated by 37 nucleotides
BPHYT_RS00255: BPHYT_RS00255 - amino acid ABC transporter, at 65,601 to 66,764
_RS00255
BPHYT_RS00260: BPHYT_RS00260 - amino acid ABC transporter, at 66,845 to 67,681
_RS00260
BPHYT_RS00265: BPHYT_RS00265 - branched-chain amino acid ABC transporter substrate-binding protein, at 67,674 to 69,488
_RS00265
BPHYT_RS00270: BPHYT_RS00270 - branched-chain amino acid ABC transporter, at 69,485 to 70,186
_RS00270
BPHYT_RS00275: BPHYT_RS00275 - urease subunit beta, at 70,224 to 70,910
_RS00275
Position (kb) 67
68
69
70 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 66.686 kb on - strand at 66.842 kb on + strand at 66.842 kb on + strand at 66.843 kb on - strand at 66.843 kb on - strand at 66.843 kb on - strand at 66.843 kb on - strand at 66.844 kb on + strand at 66.845 kb on - strand at 66.927 kb on + strand at 66.928 kb on - strand at 66.994 kb on + strand, within BPHYT_RS00260 at 66.995 kb on - strand, within BPHYT_RS00260 at 67.094 kb on + strand, within BPHYT_RS00260 at 67.236 kb on - strand, within BPHYT_RS00260 at 67.236 kb on - strand, within BPHYT_RS00260 at 67.260 kb on + strand, within BPHYT_RS00260 at 67.261 kb on - strand, within BPHYT_RS00260 at 67.592 kb on + strand, within BPHYT_RS00260 at 67.594 kb on + strand, within BPHYT_RS00260 at 67.851 kb on + strand at 67.851 kb on + strand at 67.854 kb on - strand at 67.854 kb on - strand at 67.854 kb on - strand at 67.854 kb on - strand at 67.902 kb on + strand, within BPHYT_RS00265 at 67.904 kb on + strand, within BPHYT_RS00265 at 67.918 kb on + strand, within BPHYT_RS00265 at 67.918 kb on + strand, within BPHYT_RS00265 at 67.919 kb on - strand, within BPHYT_RS00265 at 67.923 kb on - strand, within BPHYT_RS00265 at 68.058 kb on - strand, within BPHYT_RS00265 at 68.286 kb on + strand, within BPHYT_RS00265 at 68.367 kb on + strand, within BPHYT_RS00265 at 68.377 kb on - strand, within BPHYT_RS00265 at 68.413 kb on + strand, within BPHYT_RS00265 at 68.532 kb on + strand, within BPHYT_RS00265 at 68.533 kb on - strand, within BPHYT_RS00265 at 68.798 kb on - strand, within BPHYT_RS00265 at 69.074 kb on + strand, within BPHYT_RS00265 at 69.075 kb on - strand, within BPHYT_RS00265 at 69.202 kb on + strand, within BPHYT_RS00265 at 69.203 kb on - strand, within BPHYT_RS00265 at 69.286 kb on + strand, within BPHYT_RS00265 at 69.315 kb on - strand at 69.315 kb on - strand at 69.453 kb on + strand at 69.453 kb on + strand at 69.454 kb on - strand at 69.455 kb on + strand at 69.456 kb on - strand at 69.525 kb on - strand at 69.732 kb on - strand, within BPHYT_RS00270 at 69.732 kb on - strand, within BPHYT_RS00270 at 70.094 kb on + strand, within BPHYT_RS00270 at 70.095 kb on - strand, within BPHYT_RS00270 at 70.306 kb on + strand, within BPHYT_RS00275 at 70.307 kb on - strand, within BPHYT_RS00275 at 70.460 kb on - strand, within BPHYT_RS00275 at 70.460 kb on - strand, within BPHYT_RS00275 at 70.460 kb on - strand, within BPHYT_RS00275
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 66,686 - -0.2 66,842 + +0.3 66,842 + +2.1 66,843 - +0.6 66,843 - -0.5 66,843 - +1.1 66,843 - -1.0 66,844 + +0.2 66,845 - -0.2 66,927 + +0.3 66,928 - -0.8 66,994 + BPHYT_RS00260 0.18 -0.5 66,995 - BPHYT_RS00260 0.18 +1.2 67,094 + BPHYT_RS00260 0.30 -2.5 67,236 - BPHYT_RS00260 0.47 -0.3 67,236 - BPHYT_RS00260 0.47 -0.9 67,260 + BPHYT_RS00260 0.50 -1.1 67,261 - BPHYT_RS00260 0.50 -0.9 67,592 + BPHYT_RS00260 0.89 -0.2 67,594 + BPHYT_RS00260 0.89 -1.4 67,851 + -0.3 67,851 + +0.1 67,854 - +0.2 67,854 - +0.5 67,854 - -1.0 67,854 - +1.1 67,902 + BPHYT_RS00265 0.13 +0.5 67,904 + BPHYT_RS00265 0.13 +0.2 67,918 + BPHYT_RS00265 0.13 -0.3 67,918 + BPHYT_RS00265 0.13 +1.0 67,919 - BPHYT_RS00265 0.13 -0.4 67,923 - BPHYT_RS00265 0.14 -2.5 68,058 - BPHYT_RS00265 0.21 -0.6 68,286 + BPHYT_RS00265 0.34 -0.1 68,367 + BPHYT_RS00265 0.38 +0.2 68,377 - BPHYT_RS00265 0.39 -0.4 68,413 + BPHYT_RS00265 0.41 +0.2 68,532 + BPHYT_RS00265 0.47 +1.3 68,533 - BPHYT_RS00265 0.47 +3.4 68,798 - BPHYT_RS00265 0.62 +1.4 69,074 + BPHYT_RS00265 0.77 +1.4 69,075 - BPHYT_RS00265 0.77 -2.0 69,202 + BPHYT_RS00265 0.84 +0.1 69,203 - BPHYT_RS00265 0.84 -0.0 69,286 + BPHYT_RS00265 0.89 -2.4 69,315 - -1.5 69,315 - -1.0 69,453 + -0.2 69,453 + +0.4 69,454 - -0.5 69,455 + -0.0 69,456 - -0.6 69,525 - -0.7 69,732 - BPHYT_RS00270 0.35 +1.0 69,732 - BPHYT_RS00270 0.35 +0.5 70,094 + BPHYT_RS00270 0.87 -1.1 70,095 - BPHYT_RS00270 0.87 -0.9 70,306 + BPHYT_RS00275 0.12 -0.1 70,307 - BPHYT_RS00275 0.12 -3.2 70,460 - BPHYT_RS00275 0.34 +0.3 70,460 - BPHYT_RS00275 0.34 +1.2 70,460 - BPHYT_RS00275 0.34 -0.2
Or see this region's nucleotide sequence