Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00255 and BPHYT_RS00260 are separated by 80 nucleotides BPHYT_RS00260 and BPHYT_RS00265 overlap by 8 nucleotides
BPHYT_RS00255: BPHYT_RS00255 - amino acid ABC transporter, at 65,601 to 66,764
_RS00255
BPHYT_RS00260: BPHYT_RS00260 - amino acid ABC transporter, at 66,845 to 67,681
_RS00260
BPHYT_RS00265: BPHYT_RS00265 - branched-chain amino acid ABC transporter substrate-binding protein, at 67,674 to 69,488
_RS00265
Position (kb) 66
67
68 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 65.862 kb on + strand, within BPHYT_RS00255 at 65.863 kb on - strand, within BPHYT_RS00255 at 65.969 kb on + strand, within BPHYT_RS00255 at 65.970 kb on - strand, within BPHYT_RS00255 at 66.009 kb on + strand, within BPHYT_RS00255 at 66.009 kb on + strand, within BPHYT_RS00255 at 66.009 kb on + strand, within BPHYT_RS00255 at 66.010 kb on - strand, within BPHYT_RS00255 at 66.010 kb on - strand, within BPHYT_RS00255 at 66.010 kb on - strand at 66.010 kb on - strand, within BPHYT_RS00255 at 66.010 kb on - strand, within BPHYT_RS00255 at 66.015 kb on + strand, within BPHYT_RS00255 at 66.015 kb on + strand, within BPHYT_RS00255 at 66.015 kb on + strand at 66.015 kb on + strand, within BPHYT_RS00255 at 66.016 kb on - strand, within BPHYT_RS00255 at 66.016 kb on - strand, within BPHYT_RS00255 at 66.016 kb on - strand, within BPHYT_RS00255 at 66.016 kb on - strand, within BPHYT_RS00255 at 66.017 kb on + strand, within BPHYT_RS00255 at 66.017 kb on + strand, within BPHYT_RS00255 at 66.018 kb on - strand, within BPHYT_RS00255 at 66.064 kb on - strand, within BPHYT_RS00255 at 66.064 kb on - strand, within BPHYT_RS00255 at 66.153 kb on + strand, within BPHYT_RS00255 at 66.153 kb on + strand, within BPHYT_RS00255 at 66.193 kb on - strand, within BPHYT_RS00255 at 66.403 kb on - strand, within BPHYT_RS00255 at 66.420 kb on + strand, within BPHYT_RS00255 at 66.420 kb on + strand, within BPHYT_RS00255 at 66.421 kb on - strand, within BPHYT_RS00255 at 66.522 kb on + strand, within BPHYT_RS00255 at 66.522 kb on + strand, within BPHYT_RS00255 at 66.672 kb on + strand at 66.686 kb on - strand at 66.842 kb on + strand at 66.842 kb on + strand at 66.843 kb on - strand at 66.843 kb on - strand at 66.843 kb on - strand at 66.843 kb on - strand at 66.844 kb on + strand at 66.845 kb on - strand at 66.927 kb on + strand at 66.928 kb on - strand at 66.994 kb on + strand, within BPHYT_RS00260 at 66.995 kb on - strand, within BPHYT_RS00260 at 67.094 kb on + strand, within BPHYT_RS00260 at 67.236 kb on - strand, within BPHYT_RS00260 at 67.236 kb on - strand, within BPHYT_RS00260 at 67.260 kb on + strand, within BPHYT_RS00260 at 67.261 kb on - strand, within BPHYT_RS00260 at 67.592 kb on + strand, within BPHYT_RS00260 at 67.594 kb on + strand, within BPHYT_RS00260 at 67.851 kb on + strand at 67.851 kb on + strand at 67.854 kb on - strand at 67.854 kb on - strand at 67.854 kb on - strand at 67.854 kb on - strand at 67.902 kb on + strand, within BPHYT_RS00265 at 67.904 kb on + strand, within BPHYT_RS00265 at 67.918 kb on + strand, within BPHYT_RS00265 at 67.918 kb on + strand, within BPHYT_RS00265 at 67.919 kb on - strand, within BPHYT_RS00265 at 67.923 kb on - strand, within BPHYT_RS00265 at 68.058 kb on - strand, within BPHYT_RS00265 at 68.286 kb on + strand, within BPHYT_RS00265 at 68.367 kb on + strand, within BPHYT_RS00265 at 68.377 kb on - strand, within BPHYT_RS00265 at 68.413 kb on + strand, within BPHYT_RS00265 at 68.532 kb on + strand, within BPHYT_RS00265 at 68.533 kb on - strand, within BPHYT_RS00265
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 65,862 + BPHYT_RS00255 0.22 -0.8 65,863 - BPHYT_RS00255 0.23 -0.0 65,969 + BPHYT_RS00255 0.32 -1.5 65,970 - BPHYT_RS00255 0.32 -1.4 66,009 + BPHYT_RS00255 0.35 +1.8 66,009 + BPHYT_RS00255 0.35 -0.4 66,009 + BPHYT_RS00255 0.35 +1.0 66,010 - BPHYT_RS00255 0.35 -0.5 66,010 - BPHYT_RS00255 0.35 +0.1 66,010 - +1.7 66,010 - BPHYT_RS00255 0.35 +0.2 66,010 - BPHYT_RS00255 0.35 +0.2 66,015 + BPHYT_RS00255 0.36 -0.5 66,015 + BPHYT_RS00255 0.36 +1.7 66,015 + +2.1 66,015 + BPHYT_RS00255 0.36 -0.1 66,016 - BPHYT_RS00255 0.36 -0.6 66,016 - BPHYT_RS00255 0.36 +1.6 66,016 - BPHYT_RS00255 0.36 +0.7 66,016 - BPHYT_RS00255 0.36 -0.9 66,017 + BPHYT_RS00255 0.36 -0.2 66,017 + BPHYT_RS00255 0.36 -1.1 66,018 - BPHYT_RS00255 0.36 -1.7 66,064 - BPHYT_RS00255 0.40 +1.1 66,064 - BPHYT_RS00255 0.40 +0.1 66,153 + BPHYT_RS00255 0.47 -1.9 66,153 + BPHYT_RS00255 0.47 +0.1 66,193 - BPHYT_RS00255 0.51 +1.4 66,403 - BPHYT_RS00255 0.69 -2.2 66,420 + BPHYT_RS00255 0.70 -0.1 66,420 + BPHYT_RS00255 0.70 -0.1 66,421 - BPHYT_RS00255 0.70 -0.8 66,522 + BPHYT_RS00255 0.79 -2.2 66,522 + BPHYT_RS00255 0.79 +0.5 66,672 + -0.4 66,686 - -0.2 66,842 + +0.3 66,842 + +2.1 66,843 - +0.6 66,843 - -0.5 66,843 - +1.1 66,843 - -1.0 66,844 + +0.2 66,845 - -0.2 66,927 + +0.3 66,928 - -0.8 66,994 + BPHYT_RS00260 0.18 -0.5 66,995 - BPHYT_RS00260 0.18 +1.2 67,094 + BPHYT_RS00260 0.30 -2.5 67,236 - BPHYT_RS00260 0.47 -0.3 67,236 - BPHYT_RS00260 0.47 -0.9 67,260 + BPHYT_RS00260 0.50 -1.1 67,261 - BPHYT_RS00260 0.50 -0.9 67,592 + BPHYT_RS00260 0.89 -0.2 67,594 + BPHYT_RS00260 0.89 -1.4 67,851 + -0.3 67,851 + +0.1 67,854 - +0.2 67,854 - +0.5 67,854 - -1.0 67,854 - +1.1 67,902 + BPHYT_RS00265 0.13 +0.5 67,904 + BPHYT_RS00265 0.13 +0.2 67,918 + BPHYT_RS00265 0.13 -0.3 67,918 + BPHYT_RS00265 0.13 +1.0 67,919 - BPHYT_RS00265 0.13 -0.4 67,923 - BPHYT_RS00265 0.14 -2.5 68,058 - BPHYT_RS00265 0.21 -0.6 68,286 + BPHYT_RS00265 0.34 -0.1 68,367 + BPHYT_RS00265 0.38 +0.2 68,377 - BPHYT_RS00265 0.39 -0.4 68,413 + BPHYT_RS00265 0.41 +0.2 68,532 + BPHYT_RS00265 0.47 +1.3 68,533 - BPHYT_RS00265 0.47 +3.4
Or see this region's nucleotide sequence