Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00150 and BPHYT_RS00155 are separated by 302 nucleotides BPHYT_RS00155 and BPHYT_RS00160 are separated by 124 nucleotides
BPHYT_RS00150: BPHYT_RS00150 - DEAD/DEAH box helicase, at 40,276 to 41,679
_RS00150
BPHYT_RS00155: BPHYT_RS00155 - aminotransferase, at 41,982 to 43,136
_RS00155
BPHYT_RS00160: BPHYT_RS00160 - ABC transporter substrate-binding protein, at 43,261 to 44,049
_RS00160
Position (kb) 41
42
43
44 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 41.287 kb on - strand, within BPHYT_RS00150 at 41.575 kb on + strand at 41.576 kb on - strand at 41.576 kb on - strand at 41.576 kb on - strand at 41.576 kb on - strand at 41.733 kb on - strand at 41.740 kb on - strand at 41.902 kb on - strand at 41.975 kb on + strand at 41.981 kb on + strand at 41.982 kb on - strand at 42.022 kb on + strand at 42.228 kb on + strand, within BPHYT_RS00155 at 42.291 kb on + strand, within BPHYT_RS00155 at 42.337 kb on - strand, within BPHYT_RS00155 at 42.337 kb on - strand, within BPHYT_RS00155 at 42.360 kb on + strand, within BPHYT_RS00155 at 42.361 kb on - strand, within BPHYT_RS00155 at 42.591 kb on + strand, within BPHYT_RS00155 at 42.739 kb on + strand, within BPHYT_RS00155 at 42.801 kb on + strand, within BPHYT_RS00155 at 42.838 kb on - strand, within BPHYT_RS00155 at 42.838 kb on - strand, within BPHYT_RS00155 at 42.858 kb on + strand, within BPHYT_RS00155 at 42.858 kb on + strand, within BPHYT_RS00155 at 42.858 kb on + strand, within BPHYT_RS00155 at 43.044 kb on - strand at 43.052 kb on + strand at 43.140 kb on + strand at 43.141 kb on - strand at 43.173 kb on + strand at 43.173 kb on + strand at 43.185 kb on + strand at 43.185 kb on + strand at 43.185 kb on + strand at 43.456 kb on - strand, within BPHYT_RS00160 at 43.768 kb on + strand, within BPHYT_RS00160 at 43.768 kb on + strand, within BPHYT_RS00160 at 44.047 kb on + strand at 44.048 kb on - strand at 44.048 kb on - strand at 44.059 kb on + strand at 44.059 kb on + strand at 44.129 kb on + strand at 44.130 kb on - strand at 44.133 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 41,287 - BPHYT_RS00150 0.72 -1.6 41,575 + +0.2 41,576 - +2.7 41,576 - -0.4 41,576 - +0.3 41,576 - -0.1 41,733 - +1.5 41,740 - -0.5 41,902 - +1.7 41,975 + -1.9 41,981 + -1.0 41,982 - -0.6 42,022 + -0.2 42,228 + BPHYT_RS00155 0.21 +0.2 42,291 + BPHYT_RS00155 0.27 -1.9 42,337 - BPHYT_RS00155 0.31 +0.3 42,337 - BPHYT_RS00155 0.31 +0.2 42,360 + BPHYT_RS00155 0.33 +0.5 42,361 - BPHYT_RS00155 0.33 +3.0 42,591 + BPHYT_RS00155 0.53 -0.6 42,739 + BPHYT_RS00155 0.66 -1.2 42,801 + BPHYT_RS00155 0.71 -0.9 42,838 - BPHYT_RS00155 0.74 -0.2 42,838 - BPHYT_RS00155 0.74 -1.6 42,858 + BPHYT_RS00155 0.76 -1.2 42,858 + BPHYT_RS00155 0.76 -1.4 42,858 + BPHYT_RS00155 0.76 -0.2 43,044 - -0.5 43,052 + -2.0 43,140 + -0.1 43,141 - +0.7 43,173 + -3.0 43,173 + -0.3 43,185 + +2.4 43,185 + -0.5 43,185 + -1.5 43,456 - BPHYT_RS00160 0.25 -1.5 43,768 + BPHYT_RS00160 0.64 -0.4 43,768 + BPHYT_RS00160 0.64 -1.2 44,047 + +0.3 44,048 - -0.8 44,048 - +1.1 44,059 + -0.4 44,059 + -0.2 44,129 + +0.3 44,130 - +0.1 44,133 + -0.6
Or see this region's nucleotide sequence