Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00105 and BPHYT_RS00110 overlap by 4 nucleotides BPHYT_RS00110 and BPHYT_RS00115 are separated by 5 nucleotides BPHYT_RS00115 and BPHYT_RS00120 are separated by 64 nucleotides
BPHYT_RS00105: BPHYT_RS00105 - ethanolamine ammonia lyase large subunit, at 31,432 to 32,826
_RS00105
BPHYT_RS00110: BPHYT_RS00110 - ethanolamine ammonia-lyase small subunit, at 32,823 to 33,620
_RS00110
BPHYT_RS00115: BPHYT_RS00115 - acetaldehyde dehydrogenase, at 33,626 to 34,021
_RS00115
BPHYT_RS00120: BPHYT_RS00120 - aldehyde dehydrogenase, at 34,086 to 35,606
_RS00120
Position (kb) 32
33
34 Strain fitness (log2 ratio) -2
-1
0
1
2 at 31.979 kb on - strand, within BPHYT_RS00105 at 32.236 kb on + strand, within BPHYT_RS00105 at 32.237 kb on - strand, within BPHYT_RS00105 at 32.287 kb on + strand, within BPHYT_RS00105 at 32.288 kb on - strand, within BPHYT_RS00105 at 32.288 kb on - strand, within BPHYT_RS00105 at 32.360 kb on + strand, within BPHYT_RS00105 at 32.361 kb on - strand, within BPHYT_RS00105 at 32.368 kb on + strand, within BPHYT_RS00105 at 32.434 kb on + strand, within BPHYT_RS00105 at 32.441 kb on - strand, within BPHYT_RS00105 at 32.598 kb on + strand, within BPHYT_RS00105 at 32.644 kb on + strand, within BPHYT_RS00105 at 32.644 kb on + strand, within BPHYT_RS00105 at 32.907 kb on - strand, within BPHYT_RS00110 at 33.393 kb on + strand, within BPHYT_RS00110 at 33.492 kb on + strand, within BPHYT_RS00110 at 33.618 kb on + strand at 33.710 kb on + strand, within BPHYT_RS00115 at 33.813 kb on + strand, within BPHYT_RS00115 at 34.112 kb on + strand at 34.114 kb on + strand at 34.114 kb on + strand at 34.115 kb on - strand at 34.115 kb on - strand at 34.232 kb on - strand at 34.325 kb on - strand, within BPHYT_RS00120 at 34.332 kb on - strand, within BPHYT_RS00120 at 34.456 kb on + strand, within BPHYT_RS00120 at 34.457 kb on - strand, within BPHYT_RS00120 at 34.457 kb on - strand, within BPHYT_RS00120 at 34.544 kb on - strand, within BPHYT_RS00120 at 34.544 kb on - strand, within BPHYT_RS00120
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 31,979 - BPHYT_RS00105 0.39 +0.2 32,236 + BPHYT_RS00105 0.58 +0.3 32,237 - BPHYT_RS00105 0.58 +0.6 32,287 + BPHYT_RS00105 0.61 -0.5 32,288 - BPHYT_RS00105 0.61 +0.1 32,288 - BPHYT_RS00105 0.61 -0.4 32,360 + BPHYT_RS00105 0.67 -0.9 32,361 - BPHYT_RS00105 0.67 +0.0 32,368 + BPHYT_RS00105 0.67 +0.9 32,434 + BPHYT_RS00105 0.72 +2.7 32,441 - BPHYT_RS00105 0.72 +1.4 32,598 + BPHYT_RS00105 0.84 +0.8 32,644 + BPHYT_RS00105 0.87 -0.4 32,644 + BPHYT_RS00105 0.87 +0.1 32,907 - BPHYT_RS00110 0.11 +0.2 33,393 + BPHYT_RS00110 0.71 -0.1 33,492 + BPHYT_RS00110 0.84 +1.3 33,618 + +1.1 33,710 + BPHYT_RS00115 0.21 -1.2 33,813 + BPHYT_RS00115 0.47 -0.2 34,112 + -0.7 34,114 + +0.1 34,114 + -1.0 34,115 - -1.0 34,115 - -1.9 34,232 - +1.1 34,325 - BPHYT_RS00120 0.16 -0.0 34,332 - BPHYT_RS00120 0.16 -1.2 34,456 + BPHYT_RS00120 0.24 -0.3 34,457 - BPHYT_RS00120 0.24 -1.9 34,457 - BPHYT_RS00120 0.24 -0.7 34,544 - BPHYT_RS00120 0.30 -0.2 34,544 - BPHYT_RS00120 0.30 -0.0
Or see this region's nucleotide sequence