Experiment: Amylopectin from Potato; autoclaved 2.5 mg/ml (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4512 and BT4513 are separated by 4 nucleotides BT4513 and BT4514 are separated by 18 nucleotides
BT4512: BT4512 - putative zinc-type alcohol dehydrogenase (NCBI ptt file), at 5,928,828 to 5,929,880
BT4512
BT4513: BT4513 - putative rRNA methylase (NCBI ptt file), at 5,929,885 to 5,930,685
BT4513
BT4514: BT4514 - hypothetical protein (NCBI ptt file), at 5,930,704 to 5,931,522
BT4514
Position (kb)
5929
5930
5931 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5928.936 kb on - strand, within BT4512 at 5928.941 kb on + strand, within BT4512 at 5928.956 kb on + strand, within BT4512 at 5928.956 kb on + strand, within BT4512 at 5928.968 kb on + strand, within BT4512 at 5929.016 kb on - strand, within BT4512 at 5929.016 kb on - strand, within BT4512 at 5929.016 kb on - strand, within BT4512 at 5929.046 kb on + strand, within BT4512 at 5929.046 kb on + strand, within BT4512 at 5929.047 kb on - strand, within BT4512 at 5929.126 kb on - strand, within BT4512 at 5929.166 kb on - strand, within BT4512 at 5929.207 kb on + strand, within BT4512 at 5929.207 kb on + strand, within BT4512 at 5929.207 kb on + strand, within BT4512 at 5929.235 kb on + strand, within BT4512 at 5929.335 kb on - strand, within BT4512 at 5929.409 kb on + strand, within BT4512 at 5929.410 kb on - strand, within BT4512 at 5929.472 kb on - strand, within BT4512 at 5929.665 kb on + strand, within BT4512 at 5929.666 kb on - strand, within BT4512 at 5929.700 kb on - strand, within BT4512 at 5929.755 kb on - strand, within BT4512 at 5929.767 kb on - strand, within BT4512 at 5929.806 kb on + strand at 5929.807 kb on - strand at 5929.874 kb on + strand at 5929.875 kb on - strand at 5929.884 kb on + strand at 5929.885 kb on - strand at 5929.932 kb on - strand at 5929.968 kb on + strand, within BT4513 at 5930.048 kb on + strand, within BT4513 at 5930.068 kb on - strand, within BT4513 at 5930.126 kb on - strand, within BT4513 at 5930.152 kb on - strand, within BT4513 at 5930.164 kb on + strand, within BT4513 at 5930.274 kb on + strand, within BT4513 at 5930.274 kb on + strand, within BT4513 at 5930.334 kb on - strand, within BT4513 at 5930.343 kb on + strand, within BT4513 at 5930.344 kb on - strand, within BT4513 at 5930.594 kb on - strand, within BT4513 at 5930.597 kb on + strand, within BT4513 at 5930.610 kb on + strand at 5930.693 kb on - strand at 5930.751 kb on + strand at 5930.753 kb on + strand at 5930.831 kb on - strand, within BT4514 at 5930.841 kb on + strand, within BT4514 at 5930.851 kb on - strand, within BT4514 at 5930.958 kb on - strand, within BT4514 at 5930.958 kb on - strand, within BT4514 at 5930.975 kb on - strand, within BT4514 at 5931.052 kb on - strand, within BT4514 at 5931.074 kb on + strand, within BT4514 at 5931.099 kb on - strand, within BT4514 at 5931.119 kb on + strand, within BT4514 at 5931.119 kb on + strand, within BT4514 at 5931.119 kb on + strand, within BT4514 at 5931.120 kb on - strand, within BT4514 at 5931.177 kb on - strand, within BT4514 at 5931.186 kb on + strand, within BT4514 at 5931.186 kb on + strand, within BT4514 at 5931.187 kb on - strand, within BT4514 at 5931.212 kb on + strand, within BT4514 at 5931.212 kb on + strand, within BT4514 at 5931.258 kb on - strand, within BT4514 at 5931.258 kb on - strand, within BT4514 at 5931.291 kb on - strand, within BT4514 at 5931.291 kb on - strand, within BT4514 at 5931.315 kb on + strand, within BT4514 at 5931.317 kb on + strand, within BT4514 at 5931.375 kb on + strand, within BT4514 at 5931.442 kb on - strand at 5931.507 kb on + strand at 5931.507 kb on + strand at 5931.522 kb on + strand at 5931.522 kb on + strand at 5931.523 kb on - strand at 5931.523 kb on - strand at 5931.550 kb on + strand at 5931.550 kb on + strand at 5931.558 kb on - strand at 5931.611 kb on + strand at 5931.655 kb on + strand at 5931.656 kb on - strand at 5931.680 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Amylopectin from Potato; autoclaved 2.5 mg/ml (C) remove 5,928,936 - BT4512 0.10 -0.0 5,928,941 + BT4512 0.11 -0.6 5,928,956 + BT4512 0.12 -2.0 5,928,956 + BT4512 0.12 -0.5 5,928,968 + BT4512 0.13 -0.3 5,929,016 - BT4512 0.18 -1.2 5,929,016 - BT4512 0.18 -0.6 5,929,016 - BT4512 0.18 -0.0 5,929,046 + BT4512 0.21 +0.4 5,929,046 + BT4512 0.21 -1.3 5,929,047 - BT4512 0.21 -1.2 5,929,126 - BT4512 0.28 +0.4 5,929,166 - BT4512 0.32 +0.3 5,929,207 + BT4512 0.36 -1.2 5,929,207 + BT4512 0.36 -1.9 5,929,207 + BT4512 0.36 +0.6 5,929,235 + BT4512 0.39 -0.6 5,929,335 - BT4512 0.48 -2.2 5,929,409 + BT4512 0.55 -2.4 5,929,410 - BT4512 0.55 +1.1 5,929,472 - BT4512 0.61 -1.9 5,929,665 + BT4512 0.79 -0.6 5,929,666 - BT4512 0.80 -1.9 5,929,700 - BT4512 0.83 -1.2 5,929,755 - BT4512 0.88 +1.4 5,929,767 - BT4512 0.89 -0.6 5,929,806 + +1.7 5,929,807 - -1.1 5,929,874 + -1.6 5,929,875 - -1.6 5,929,884 + +0.4 5,929,885 - +0.4 5,929,932 - +0.1 5,929,968 + BT4513 0.10 -1.2 5,930,048 + BT4513 0.20 -0.9 5,930,068 - BT4513 0.23 -0.3 5,930,126 - BT4513 0.30 -1.3 5,930,152 - BT4513 0.33 -2.2 5,930,164 + BT4513 0.35 -2.2 5,930,274 + BT4513 0.49 +0.4 5,930,274 + BT4513 0.49 -1.4 5,930,334 - BT4513 0.56 -1.2 5,930,343 + BT4513 0.57 -2.3 5,930,344 - BT4513 0.57 -1.9 5,930,594 - BT4513 0.89 -0.6 5,930,597 + BT4513 0.89 -1.5 5,930,610 + -0.6 5,930,693 - -1.6 5,930,751 + -0.2 5,930,753 + -1.9 5,930,831 - BT4514 0.16 +0.7 5,930,841 + BT4514 0.17 +0.4 5,930,851 - BT4514 0.18 -0.6 5,930,958 - BT4514 0.31 -0.6 5,930,958 - BT4514 0.31 -0.6 5,930,975 - BT4514 0.33 +0.4 5,931,052 - BT4514 0.42 -0.2 5,931,074 + BT4514 0.45 -0.6 5,931,099 - BT4514 0.48 -1.5 5,931,119 + BT4514 0.51 +1.4 5,931,119 + BT4514 0.51 -0.6 5,931,119 + BT4514 0.51 -0.4 5,931,120 - BT4514 0.51 -2.1 5,931,177 - BT4514 0.58 -2.3 5,931,186 + BT4514 0.59 -0.3 5,931,186 + BT4514 0.59 -0.3 5,931,187 - BT4514 0.59 -3.1 5,931,212 + BT4514 0.62 -1.2 5,931,212 + BT4514 0.62 -1.2 5,931,258 - BT4514 0.68 +0.4 5,931,258 - BT4514 0.68 +0.4 5,931,291 - BT4514 0.72 -0.9 5,931,291 - BT4514 0.72 -0.6 5,931,315 + BT4514 0.75 -1.9 5,931,317 + BT4514 0.75 -1.0 5,931,375 + BT4514 0.82 +2.0 5,931,442 - -0.6 5,931,507 + -0.6 5,931,507 + -1.4 5,931,522 + -1.4 5,931,522 + -0.6 5,931,523 - +0.4 5,931,523 - -0.6 5,931,550 + -2.4 5,931,550 + -0.2 5,931,558 - -2.4 5,931,611 + -0.7 5,931,655 + -1.6 5,931,656 - -0.2 5,931,680 - +0.4
Or see this region's nucleotide sequence