Experiment: Amylopectin from Potato; autoclaved 2.5 mg/ml (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3502 and BT3503 are separated by 231 nucleotides BT3503 and BT3504 are separated by 57 nucleotides BT3504 and BT3505 are separated by 16 nucleotides
BT3502: BT3502 - hypothetical protein (NCBI ptt file), at 4,517,092 to 4,517,367
BT3502
BT3503: BT3503 - glutaminase A (NCBI ptt file), at 4,517,599 to 4,518,114
BT3503
BT3504: BT3504 - hypothetical protein (NCBI ptt file), at 4,518,172 to 4,519,728
BT3504
BT3505: BT3505 - putative outer membrane protein, probably involved in nutrient binding (NCBI ptt file), at 4,519,745 to 4,522,867
BT3505
Position (kb)
4518
4519
4520 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4517.172 kb on - strand, within BT3502 at 4517.225 kb on + strand, within BT3502 at 4517.237 kb on - strand, within BT3502 at 4517.237 kb on - strand, within BT3502 at 4517.239 kb on - strand at 4517.239 kb on - strand, within BT3502 at 4517.250 kb on - strand, within BT3502 at 4517.332 kb on - strand, within BT3502 at 4517.377 kb on + strand at 4517.378 kb on - strand at 4517.381 kb on + strand at 4517.386 kb on + strand at 4517.426 kb on - strand at 4517.475 kb on - strand at 4517.494 kb on + strand at 4517.538 kb on - strand at 4517.981 kb on - strand, within BT3503 at 4518.132 kb on - strand at 4518.182 kb on + strand at 4518.235 kb on - strand at 4518.257 kb on + strand at 4518.258 kb on - strand at 4518.338 kb on + strand, within BT3504 at 4518.339 kb on - strand, within BT3504 at 4518.414 kb on + strand, within BT3504 at 4518.415 kb on - strand, within BT3504 at 4518.434 kb on - strand, within BT3504 at 4518.440 kb on - strand, within BT3504 at 4518.489 kb on - strand, within BT3504 at 4518.534 kb on + strand, within BT3504 at 4518.534 kb on + strand, within BT3504 at 4518.539 kb on - strand, within BT3504 at 4518.557 kb on + strand, within BT3504 at 4518.561 kb on + strand, within BT3504 at 4518.703 kb on - strand, within BT3504 at 4518.720 kb on + strand at 4518.721 kb on - strand, within BT3504 at 4518.727 kb on - strand, within BT3504 at 4518.780 kb on + strand, within BT3504 at 4518.784 kb on - strand, within BT3504 at 4518.784 kb on - strand, within BT3504 at 4518.820 kb on - strand, within BT3504 at 4518.827 kb on + strand, within BT3504 at 4518.828 kb on - strand, within BT3504 at 4518.828 kb on - strand, within BT3504 at 4518.836 kb on - strand, within BT3504 at 4518.853 kb on + strand, within BT3504 at 4518.853 kb on + strand, within BT3504 at 4518.856 kb on - strand, within BT3504 at 4518.856 kb on - strand, within BT3504 at 4518.863 kb on + strand, within BT3504 at 4518.875 kb on - strand, within BT3504 at 4518.906 kb on - strand, within BT3504 at 4518.906 kb on - strand, within BT3504 at 4518.907 kb on + strand, within BT3504 at 4518.909 kb on + strand, within BT3504 at 4518.913 kb on - strand, within BT3504 at 4518.985 kb on - strand, within BT3504 at 4518.993 kb on + strand, within BT3504 at 4518.997 kb on - strand, within BT3504 at 4519.022 kb on - strand, within BT3504 at 4519.030 kb on - strand, within BT3504 at 4519.033 kb on + strand, within BT3504 at 4519.110 kb on + strand, within BT3504 at 4519.145 kb on + strand, within BT3504 at 4519.146 kb on - strand, within BT3504 at 4519.204 kb on + strand, within BT3504 at 4519.235 kb on - strand, within BT3504 at 4519.235 kb on - strand, within BT3504 at 4519.235 kb on - strand, within BT3504 at 4519.276 kb on - strand, within BT3504 at 4519.306 kb on - strand, within BT3504 at 4519.320 kb on + strand, within BT3504 at 4519.347 kb on + strand, within BT3504 at 4519.347 kb on + strand, within BT3504 at 4519.348 kb on - strand, within BT3504 at 4519.354 kb on - strand, within BT3504 at 4519.449 kb on + strand, within BT3504 at 4519.449 kb on + strand, within BT3504 at 4519.450 kb on - strand, within BT3504 at 4519.472 kb on + strand, within BT3504 at 4519.574 kb on + strand at 4519.574 kb on + strand at 4519.617 kb on - strand at 4519.635 kb on + strand at 4519.689 kb on + strand at 4519.745 kb on - strand at 4519.956 kb on + strand at 4520.023 kb on - strand at 4520.049 kb on + strand at 4520.067 kb on + strand, within BT3505 at 4520.075 kb on - strand, within BT3505 at 4520.112 kb on + strand, within BT3505 at 4520.159 kb on - strand, within BT3505 at 4520.159 kb on - strand, within BT3505 at 4520.194 kb on + strand, within BT3505 at 4520.247 kb on + strand, within BT3505 at 4520.285 kb on + strand, within BT3505 at 4520.288 kb on - strand, within BT3505 at 4520.288 kb on - strand, within BT3505 at 4520.329 kb on + strand, within BT3505 at 4520.383 kb on + strand, within BT3505 at 4520.384 kb on - strand, within BT3505 at 4520.384 kb on - strand, within BT3505 at 4520.384 kb on - strand, within BT3505 at 4520.388 kb on - strand, within BT3505 at 4520.388 kb on - strand, within BT3505 at 4520.415 kb on + strand, within BT3505 at 4520.415 kb on + strand, within BT3505 at 4520.423 kb on - strand, within BT3505 at 4520.477 kb on - strand, within BT3505 at 4520.480 kb on + strand, within BT3505 at 4520.481 kb on - strand, within BT3505 at 4520.482 kb on + strand, within BT3505 at 4520.486 kb on - strand, within BT3505 at 4520.495 kb on - strand, within BT3505 at 4520.599 kb on - strand, within BT3505 at 4520.659 kb on - strand, within BT3505
Per-strain Table
Position Strand Gene LocusTag Fraction Amylopectin from Potato; autoclaved 2.5 mg/ml (C) remove 4,517,172 - BT3502 0.29 -0.6 4,517,225 + BT3502 0.48 +0.4 4,517,237 - BT3502 0.53 -1.2 4,517,237 - BT3502 0.53 -1.3 4,517,239 - -1.2 4,517,239 - BT3502 0.53 +0.0 4,517,250 - BT3502 0.57 +0.4 4,517,332 - BT3502 0.87 -0.6 4,517,377 + -1.6 4,517,378 - -1.6 4,517,381 + +0.8 4,517,386 + -0.6 4,517,426 - +1.4 4,517,475 - +0.4 4,517,494 + -0.6 4,517,538 - +2.4 4,517,981 - BT3503 0.74 -1.5 4,518,132 - -1.2 4,518,182 + -0.6 4,518,235 - -1.2 4,518,257 + -0.6 4,518,258 - -0.6 4,518,338 + BT3504 0.11 +0.4 4,518,339 - BT3504 0.11 +0.4 4,518,414 + BT3504 0.16 -0.6 4,518,415 - BT3504 0.16 -2.0 4,518,434 - BT3504 0.17 -0.9 4,518,440 - BT3504 0.17 -2.5 4,518,489 - BT3504 0.20 -2.2 4,518,534 + BT3504 0.23 -1.6 4,518,534 + BT3504 0.23 +0.4 4,518,539 - BT3504 0.24 +0.4 4,518,557 + BT3504 0.25 -2.0 4,518,561 + BT3504 0.25 +0.4 4,518,703 - BT3504 0.34 -1.6 4,518,720 + +0.4 4,518,721 - BT3504 0.35 -0.2 4,518,727 - BT3504 0.36 -0.9 4,518,780 + BT3504 0.39 +0.4 4,518,784 - BT3504 0.39 -0.6 4,518,784 - BT3504 0.39 -2.8 4,518,820 - BT3504 0.42 -0.3 4,518,827 + BT3504 0.42 +0.4 4,518,828 - BT3504 0.42 +1.4 4,518,828 - BT3504 0.42 -2.5 4,518,836 - BT3504 0.43 -1.3 4,518,853 + BT3504 0.44 +0.4 4,518,853 + BT3504 0.44 -0.2 4,518,856 - BT3504 0.44 -1.2 4,518,856 - BT3504 0.44 -0.6 4,518,863 + BT3504 0.44 -1.6 4,518,875 - BT3504 0.45 -0.6 4,518,906 - BT3504 0.47 -3.5 4,518,906 - BT3504 0.47 -3.4 4,518,907 + BT3504 0.47 -0.2 4,518,909 + BT3504 0.47 -0.6 4,518,913 - BT3504 0.48 -3.6 4,518,985 - BT3504 0.52 -2.8 4,518,993 + BT3504 0.53 -1.3 4,518,997 - BT3504 0.53 -1.2 4,519,022 - BT3504 0.55 +0.0 4,519,030 - BT3504 0.55 -1.6 4,519,033 + BT3504 0.55 +0.4 4,519,110 + BT3504 0.60 -1.5 4,519,145 + BT3504 0.62 -0.6 4,519,146 - BT3504 0.63 -1.3 4,519,204 + BT3504 0.66 -2.6 4,519,235 - BT3504 0.68 -2.6 4,519,235 - BT3504 0.68 -1.1 4,519,235 - BT3504 0.68 -1.3 4,519,276 - BT3504 0.71 +0.4 4,519,306 - BT3504 0.73 -2.2 4,519,320 + BT3504 0.74 -1.8 4,519,347 + BT3504 0.75 -1.6 4,519,347 + BT3504 0.75 -0.6 4,519,348 - BT3504 0.76 +1.4 4,519,354 - BT3504 0.76 -1.7 4,519,449 + BT3504 0.82 -0.9 4,519,449 + BT3504 0.82 -2.2 4,519,450 - BT3504 0.82 +0.4 4,519,472 + BT3504 0.83 -1.2 4,519,574 + +0.4 4,519,574 + -1.2 4,519,617 - +1.0 4,519,635 + -0.8 4,519,689 + -1.4 4,519,745 - -1.4 4,519,956 + +0.4 4,520,023 - -1.2 4,520,049 + -1.2 4,520,067 + BT3505 0.10 -1.0 4,520,075 - BT3505 0.11 -1.6 4,520,112 + BT3505 0.12 -1.9 4,520,159 - BT3505 0.13 -2.9 4,520,159 - BT3505 0.13 +0.4 4,520,194 + BT3505 0.14 +0.4 4,520,247 + BT3505 0.16 -1.0 4,520,285 + BT3505 0.17 -1.2 4,520,288 - BT3505 0.17 +0.4 4,520,288 - BT3505 0.17 -0.2 4,520,329 + BT3505 0.19 -0.2 4,520,383 + BT3505 0.20 -1.3 4,520,384 - BT3505 0.20 -0.7 4,520,384 - BT3505 0.20 -0.4 4,520,384 - BT3505 0.20 -2.7 4,520,388 - BT3505 0.21 -1.0 4,520,388 - BT3505 0.21 -0.6 4,520,415 + BT3505 0.21 -0.2 4,520,415 + BT3505 0.21 -2.2 4,520,423 - BT3505 0.22 -1.9 4,520,477 - BT3505 0.23 -0.8 4,520,480 + BT3505 0.24 -0.6 4,520,481 - BT3505 0.24 -1.3 4,520,482 + BT3505 0.24 -0.5 4,520,486 - BT3505 0.24 -0.8 4,520,495 - BT3505 0.24 -2.2 4,520,599 - BT3505 0.27 -1.7 4,520,659 - BT3505 0.29 -2.8
Or see this region's nucleotide sequence