Experiment: Amylopectin from Potato; autoclaved 2.5 mg/ml (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3182 and BT3183 are separated by 81 nucleotides BT3183 and BT3184 are separated by 2 nucleotides BT3184 and BT3185 are separated by 107 nucleotides BT3185 and BT3186 are separated by 11 nucleotides
BT3182: BT3182 - rubrerythrin (NCBI ptt file), at 4,071,799 to 4,072,377
BT3182
BT3183: BT3183 - hypothetical protein (NCBI ptt file), at 4,072,459 to 4,072,731
BT3183
BT3184: BT3184 - L-aspartate oxidase (NCBI ptt file), at 4,072,734 to 4,074,305
BT3184
BT3185: BT3185 - hypothetical protein (NCBI ptt file), at 4,074,413 to 4,074,808
BT3185
BT3186: BT3186 - dihydrolipoamide dehydrogenase (NCBI ptt file), at 4,074,820 to 4,076,163
BT3186
Position (kb)
4072
4073
4074
4075 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 4071.744 kb on - strand at 4071.747 kb on + strand at 4071.773 kb on - strand at 4071.786 kb on - strand at 4071.786 kb on - strand at 4071.792 kb on + strand at 4071.798 kb on + strand at 4071.804 kb on + strand at 4071.813 kb on + strand at 4071.814 kb on - strand at 4071.870 kb on + strand, within BT3182 at 4071.870 kb on + strand, within BT3182 at 4071.870 kb on + strand, within BT3182 at 4071.871 kb on - strand, within BT3182 at 4071.871 kb on - strand, within BT3182 at 4072.002 kb on + strand, within BT3182 at 4072.002 kb on + strand, within BT3182 at 4072.002 kb on + strand, within BT3182 at 4072.002 kb on + strand, within BT3182 at 4072.003 kb on - strand, within BT3182 at 4072.003 kb on - strand, within BT3182 at 4072.007 kb on + strand, within BT3182 at 4072.014 kb on + strand, within BT3182 at 4072.044 kb on + strand, within BT3182 at 4072.044 kb on + strand, within BT3182 at 4072.047 kb on + strand, within BT3182 at 4072.048 kb on - strand, within BT3182 at 4072.048 kb on - strand, within BT3182 at 4072.158 kb on + strand, within BT3182 at 4072.162 kb on + strand, within BT3182 at 4072.241 kb on + strand, within BT3182 at 4072.241 kb on + strand, within BT3182 at 4072.241 kb on + strand, within BT3182 at 4072.289 kb on - strand, within BT3182 at 4072.291 kb on - strand, within BT3182 at 4072.349 kb on - strand at 4072.375 kb on + strand at 4072.376 kb on - strand at 4072.381 kb on + strand at 4072.382 kb on - strand at 4072.382 kb on - strand at 4072.395 kb on + strand at 4072.396 kb on - strand at 4072.402 kb on + strand at 4072.420 kb on - strand at 4072.420 kb on - strand at 4072.470 kb on + strand at 4072.471 kb on - strand at 4072.471 kb on - strand at 4072.506 kb on + strand, within BT3183 at 4072.521 kb on + strand, within BT3183 at 4072.522 kb on - strand, within BT3183 at 4072.550 kb on + strand, within BT3183 at 4072.550 kb on + strand, within BT3183 at 4072.551 kb on - strand, within BT3183 at 4072.551 kb on - strand, within BT3183 at 4072.556 kb on + strand, within BT3183 at 4072.556 kb on + strand, within BT3183 at 4072.556 kb on + strand, within BT3183 at 4072.580 kb on + strand, within BT3183 at 4072.611 kb on - strand, within BT3183 at 4072.662 kb on + strand, within BT3183 at 4072.671 kb on - strand, within BT3183 at 4072.681 kb on + strand, within BT3183 at 4072.682 kb on - strand, within BT3183 at 4072.707 kb on + strand at 4072.707 kb on - strand at 4072.708 kb on - strand at 4072.711 kb on + strand at 4072.712 kb on - strand at 4072.716 kb on + strand at 4072.717 kb on - strand at 4072.763 kb on + strand at 4072.763 kb on + strand at 4072.763 kb on + strand at 4072.766 kb on - strand at 4072.766 kb on - strand at 4072.816 kb on + strand at 4072.821 kb on + strand at 4072.821 kb on + strand at 4072.863 kb on + strand at 4072.952 kb on + strand, within BT3184 at 4072.956 kb on + strand, within BT3184 at 4072.956 kb on + strand, within BT3184 at 4072.956 kb on + strand, within BT3184 at 4072.956 kb on + strand, within BT3184 at 4072.956 kb on + strand, within BT3184 at 4072.957 kb on - strand, within BT3184 at 4072.960 kb on + strand, within BT3184 at 4072.960 kb on + strand, within BT3184 at 4072.960 kb on + strand, within BT3184 at 4072.961 kb on - strand, within BT3184 at 4072.961 kb on - strand, within BT3184 at 4072.961 kb on - strand, within BT3184 at 4072.961 kb on - strand, within BT3184 at 4072.961 kb on - strand, within BT3184 at 4072.961 kb on - strand, within BT3184 at 4072.965 kb on - strand, within BT3184 at 4072.965 kb on - strand, within BT3184 at 4073.008 kb on - strand, within BT3184 at 4073.008 kb on - strand, within BT3184 at 4073.072 kb on - strand, within BT3184 at 4073.094 kb on - strand, within BT3184 at 4073.117 kb on - strand, within BT3184 at 4073.119 kb on - strand, within BT3184 at 4073.197 kb on + strand, within BT3184 at 4073.255 kb on - strand, within BT3184 at 4073.282 kb on - strand, within BT3184 at 4073.324 kb on - strand, within BT3184 at 4073.381 kb on - strand, within BT3184 at 4073.381 kb on - strand, within BT3184 at 4073.468 kb on - strand, within BT3184 at 4073.505 kb on - strand, within BT3184 at 4073.565 kb on + strand, within BT3184 at 4073.566 kb on + strand, within BT3184 at 4073.566 kb on + strand, within BT3184 at 4073.612 kb on + strand, within BT3184 at 4073.627 kb on - strand, within BT3184 at 4073.691 kb on + strand, within BT3184 at 4073.709 kb on - strand, within BT3184 at 4073.728 kb on + strand, within BT3184 at 4073.729 kb on - strand, within BT3184 at 4073.729 kb on - strand, within BT3184 at 4073.732 kb on + strand, within BT3184 at 4073.732 kb on + strand, within BT3184 at 4073.732 kb on + strand, within BT3184 at 4073.732 kb on + strand, within BT3184 at 4073.732 kb on + strand, within BT3184 at 4073.732 kb on + strand, within BT3184 at 4073.764 kb on + strand, within BT3184 at 4073.833 kb on - strand, within BT3184 at 4073.924 kb on + strand, within BT3184 at 4073.925 kb on - strand, within BT3184 at 4073.931 kb on - strand, within BT3184 at 4074.131 kb on + strand, within BT3184 at 4074.182 kb on + strand at 4074.182 kb on + strand at 4074.183 kb on - strand at 4074.183 kb on - strand at 4074.213 kb on - strand at 4074.301 kb on - strand at 4074.340 kb on + strand at 4074.477 kb on + strand, within BT3185 at 4074.507 kb on - strand, within BT3185 at 4074.546 kb on + strand, within BT3185 at 4074.562 kb on + strand, within BT3185 at 4074.563 kb on - strand, within BT3185 at 4074.575 kb on + strand, within BT3185 at 4074.643 kb on + strand, within BT3185 at 4074.643 kb on + strand, within BT3185 at 4074.643 kb on + strand, within BT3185 at 4074.662 kb on - strand, within BT3185 at 4074.710 kb on + strand, within BT3185 at 4074.814 kb on - strand at 4074.819 kb on + strand at 4074.827 kb on - strand at 4074.864 kb on + strand at 4074.864 kb on + strand at 4074.864 kb on + strand at 4074.865 kb on - strand at 4074.865 kb on - strand at 4074.865 kb on - strand at 4074.902 kb on + strand at 4074.921 kb on + strand at 4074.992 kb on - strand, within BT3186 at 4075.098 kb on - strand, within BT3186 at 4075.164 kb on - strand, within BT3186 at 4075.167 kb on + strand, within BT3186 at 4075.168 kb on - strand, within BT3186 at 4075.199 kb on - strand, within BT3186
Per-strain Table
Position Strand Gene LocusTag Fraction Amylopectin from Potato; autoclaved 2.5 mg/ml (C) remove 4,071,744 - -0.9 4,071,747 + -1.5 4,071,773 - -0.6 4,071,786 - -0.6 4,071,786 - -1.9 4,071,792 + -1.2 4,071,798 + -1.1 4,071,804 + -1.3 4,071,813 + +0.4 4,071,814 - -2.4 4,071,870 + BT3182 0.12 -1.2 4,071,870 + BT3182 0.12 -1.0 4,071,870 + BT3182 0.12 -1.4 4,071,871 - BT3182 0.12 +0.1 4,071,871 - BT3182 0.12 -0.2 4,072,002 + BT3182 0.35 -1.6 4,072,002 + BT3182 0.35 +0.4 4,072,002 + BT3182 0.35 -3.0 4,072,002 + BT3182 0.35 -3.4 4,072,003 - BT3182 0.35 +0.8 4,072,003 - BT3182 0.35 -0.6 4,072,007 + BT3182 0.36 -1.9 4,072,014 + BT3182 0.37 +0.4 4,072,044 + BT3182 0.42 -1.4 4,072,044 + BT3182 0.42 -1.1 4,072,047 + BT3182 0.43 +0.4 4,072,048 - BT3182 0.43 -1.2 4,072,048 - BT3182 0.43 +0.4 4,072,158 + BT3182 0.62 -0.8 4,072,162 + BT3182 0.63 -1.6 4,072,241 + BT3182 0.76 -1.1 4,072,241 + BT3182 0.76 +0.4 4,072,241 + BT3182 0.76 -1.1 4,072,289 - BT3182 0.85 -0.6 4,072,291 - BT3182 0.85 -0.8 4,072,349 - -2.4 4,072,375 + +2.0 4,072,376 - -0.8 4,072,381 + -0.9 4,072,382 - -0.7 4,072,382 - -2.9 4,072,395 + +0.0 4,072,396 - -0.8 4,072,402 + -0.6 4,072,420 - -2.6 4,072,420 - -1.4 4,072,470 + -1.2 4,072,471 - -1.0 4,072,471 - -1.2 4,072,506 + BT3183 0.17 -0.3 4,072,521 + BT3183 0.23 -1.2 4,072,522 - BT3183 0.23 -1.6 4,072,550 + BT3183 0.33 -1.2 4,072,550 + BT3183 0.33 -2.4 4,072,551 - BT3183 0.34 -0.2 4,072,551 - BT3183 0.34 -1.2 4,072,556 + BT3183 0.36 +0.0 4,072,556 + BT3183 0.36 -0.6 4,072,556 + BT3183 0.36 -1.2 4,072,580 + BT3183 0.44 +0.4 4,072,611 - BT3183 0.56 -1.3 4,072,662 + BT3183 0.74 -1.6 4,072,671 - BT3183 0.78 -2.0 4,072,681 + BT3183 0.81 +0.4 4,072,682 - BT3183 0.82 -0.6 4,072,707 + -2.2 4,072,707 - -1.2 4,072,708 - -1.6 4,072,711 + +0.4 4,072,712 - -2.2 4,072,716 + -1.6 4,072,717 - -3.0 4,072,763 + -3.8 4,072,763 + -3.0 4,072,763 + -1.6 4,072,766 - -4.2 4,072,766 - -3.8 4,072,816 + -2.6 4,072,821 + -1.2 4,072,821 + -1.7 4,072,863 + -3.4 4,072,952 + BT3184 0.14 -2.4 4,072,956 + BT3184 0.14 -2.9 4,072,956 + BT3184 0.14 -0.6 4,072,956 + BT3184 0.14 -2.7 4,072,956 + BT3184 0.14 -2.4 4,072,956 + BT3184 0.14 -3.9 4,072,957 - BT3184 0.14 -0.6 4,072,960 + BT3184 0.14 -2.7 4,072,960 + BT3184 0.14 -1.7 4,072,960 + BT3184 0.14 -2.4 4,072,961 - BT3184 0.14 -4.0 4,072,961 - BT3184 0.14 -2.8 4,072,961 - BT3184 0.14 -2.1 4,072,961 - BT3184 0.14 -4.8 4,072,961 - BT3184 0.14 -1.3 4,072,961 - BT3184 0.14 -5.5 4,072,965 - BT3184 0.15 -3.1 4,072,965 - BT3184 0.15 -4.7 4,073,008 - BT3184 0.17 -3.6 4,073,008 - BT3184 0.17 -3.0 4,073,072 - BT3184 0.22 -3.5 4,073,094 - BT3184 0.23 -2.6 4,073,117 - BT3184 0.24 -1.2 4,073,119 - BT3184 0.24 -1.2 4,073,197 + BT3184 0.29 -2.0 4,073,255 - BT3184 0.33 -1.9 4,073,282 - BT3184 0.35 -1.2 4,073,324 - BT3184 0.38 -2.2 4,073,381 - BT3184 0.41 -2.7 4,073,381 - BT3184 0.41 -3.9 4,073,468 - BT3184 0.47 -2.2 4,073,505 - BT3184 0.49 -4.4 4,073,565 + BT3184 0.53 -1.2 4,073,566 + BT3184 0.53 -4.3 4,073,566 + BT3184 0.53 -1.6 4,073,612 + BT3184 0.56 -0.6 4,073,627 - BT3184 0.57 -2.4 4,073,691 + BT3184 0.61 -0.9 4,073,709 - BT3184 0.62 -2.4 4,073,728 + BT3184 0.63 -3.4 4,073,729 - BT3184 0.63 -2.6 4,073,729 - BT3184 0.63 -1.6 4,073,732 + BT3184 0.63 -2.4 4,073,732 + BT3184 0.63 -0.6 4,073,732 + BT3184 0.63 -2.4 4,073,732 + BT3184 0.63 -2.7 4,073,732 + BT3184 0.63 -3.8 4,073,732 + BT3184 0.63 -0.6 4,073,764 + BT3184 0.66 -5.5 4,073,833 - BT3184 0.70 -4.4 4,073,924 + BT3184 0.76 -1.6 4,073,925 - BT3184 0.76 -1.8 4,073,931 - BT3184 0.76 -3.7 4,074,131 + BT3184 0.89 -4.9 4,074,182 + -2.9 4,074,182 + -2.4 4,074,183 - -3.5 4,074,183 - -2.4 4,074,213 - -2.7 4,074,301 - -2.7 4,074,340 + -0.2 4,074,477 + BT3185 0.16 -0.3 4,074,507 - BT3185 0.24 -1.0 4,074,546 + BT3185 0.34 -0.7 4,074,562 + BT3185 0.38 -1.2 4,074,563 - BT3185 0.38 -1.5 4,074,575 + BT3185 0.41 -1.2 4,074,643 + BT3185 0.58 -0.6 4,074,643 + BT3185 0.58 -1.4 4,074,643 + BT3185 0.58 -0.2 4,074,662 - BT3185 0.63 -1.1 4,074,710 + BT3185 0.75 -0.2 4,074,814 - -1.0 4,074,819 + -0.6 4,074,827 - -0.8 4,074,864 + -2.0 4,074,864 + -1.2 4,074,864 + -1.9 4,074,865 - -2.0 4,074,865 - -0.9 4,074,865 - -1.4 4,074,902 + -1.4 4,074,921 + -0.4 4,074,992 - BT3186 0.13 -0.5 4,075,098 - BT3186 0.21 -1.2 4,075,164 - BT3186 0.26 +0.4 4,075,167 + BT3186 0.26 -0.9 4,075,168 - BT3186 0.26 -1.0 4,075,199 - BT3186 0.28 -1.4
Or see this region's nucleotide sequence