Experiment: Amylopectin from Potato; autoclaved 2.5 mg/ml (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3072 and BT3073 are separated by 137 nucleotides BT3073 and BT3074 are separated by 2 nucleotides BT3074 and BT3075 overlap by 10 nucleotides
BT3072: BT3072 - collagenase precursor (NCBI ptt file), at 3,895,716 to 3,896,999
BT3072
BT3073: BT3073 - putative TIM-barrel enzyme, possible dehydrogenase, contains a highly conserved dihydrouridine synthase domain (NCBI ptt file), at 3,897,137 to 3,898,129
BT3073
BT3074: BT3074 - conserved hypothetical protein (NCBI ptt file), at 3,898,132 to 3,899,097
BT3074
BT3075: BT3075 - NAD-dependent epimerase/dehydratase family protein (NCBI ptt file), at 3,899,088 to 3,900,095
BT3075
Position (kb)
3897
3898
3899 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3896.157 kb on - strand, within BT3072 at 3896.188 kb on + strand, within BT3072 at 3896.204 kb on + strand, within BT3072 at 3896.213 kb on - strand, within BT3072 at 3896.213 kb on - strand, within BT3072 at 3896.391 kb on - strand, within BT3072 at 3896.391 kb on - strand, within BT3072 at 3896.438 kb on - strand, within BT3072 at 3896.519 kb on + strand, within BT3072 at 3896.520 kb on - strand, within BT3072 at 3896.543 kb on - strand, within BT3072 at 3896.683 kb on + strand, within BT3072 at 3896.774 kb on + strand, within BT3072 at 3896.780 kb on - strand, within BT3072 at 3896.783 kb on + strand, within BT3072 at 3896.790 kb on - strand, within BT3072 at 3896.839 kb on - strand, within BT3072 at 3897.145 kb on - strand at 3897.145 kb on - strand at 3897.151 kb on + strand at 3897.153 kb on + strand at 3897.153 kb on + strand at 3897.153 kb on + strand at 3897.153 kb on + strand at 3897.172 kb on + strand at 3897.172 kb on + strand at 3897.249 kb on + strand, within BT3073 at 3897.249 kb on + strand, within BT3073 at 3897.249 kb on + strand, within BT3073 at 3897.250 kb on - strand, within BT3073 at 3897.252 kb on - strand, within BT3073 at 3897.354 kb on - strand, within BT3073 at 3897.359 kb on - strand, within BT3073 at 3897.454 kb on - strand, within BT3073 at 3897.541 kb on - strand, within BT3073 at 3897.564 kb on + strand, within BT3073 at 3897.584 kb on - strand, within BT3073 at 3897.613 kb on - strand, within BT3073 at 3897.669 kb on - strand, within BT3073 at 3897.669 kb on - strand, within BT3073 at 3897.733 kb on + strand, within BT3073 at 3897.734 kb on - strand, within BT3073 at 3897.734 kb on - strand, within BT3073 at 3897.919 kb on - strand, within BT3073 at 3898.042 kb on + strand at 3898.134 kb on - strand at 3898.138 kb on - strand at 3898.188 kb on - strand at 3898.248 kb on - strand, within BT3074 at 3898.249 kb on + strand, within BT3074 at 3898.250 kb on - strand, within BT3074 at 3898.274 kb on + strand, within BT3074 at 3898.323 kb on + strand, within BT3074 at 3898.330 kb on - strand, within BT3074 at 3898.450 kb on + strand, within BT3074 at 3898.451 kb on - strand, within BT3074 at 3898.577 kb on + strand, within BT3074 at 3898.578 kb on - strand, within BT3074 at 3898.581 kb on + strand, within BT3074 at 3898.601 kb on - strand, within BT3074 at 3898.639 kb on - strand, within BT3074 at 3898.677 kb on - strand, within BT3074 at 3898.743 kb on + strand, within BT3074 at 3898.743 kb on + strand, within BT3074 at 3898.764 kb on + strand, within BT3074 at 3898.779 kb on + strand, within BT3074 at 3899.025 kb on - strand at 3899.036 kb on - strand at 3899.083 kb on + strand at 3899.084 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Amylopectin from Potato; autoclaved 2.5 mg/ml (C) remove 3,896,157 - BT3072 0.34 -2.1 3,896,188 + BT3072 0.37 -0.6 3,896,204 + BT3072 0.38 -1.1 3,896,213 - BT3072 0.39 -1.1 3,896,213 - BT3072 0.39 +0.4 3,896,391 - BT3072 0.53 -0.9 3,896,391 - BT3072 0.53 -0.6 3,896,438 - BT3072 0.56 -2.4 3,896,519 + BT3072 0.63 +0.4 3,896,520 - BT3072 0.63 -0.6 3,896,543 - BT3072 0.64 -2.4 3,896,683 + BT3072 0.75 -2.1 3,896,774 + BT3072 0.82 -1.6 3,896,780 - BT3072 0.83 -3.9 3,896,783 + BT3072 0.83 -1.1 3,896,790 - BT3072 0.84 -0.9 3,896,839 - BT3072 0.87 +0.4 3,897,145 - -1.9 3,897,145 - -2.1 3,897,151 + -1.6 3,897,153 + -0.5 3,897,153 + -2.7 3,897,153 + -1.3 3,897,153 + -3.0 3,897,172 + -3.0 3,897,172 + -0.6 3,897,249 + BT3073 0.11 -2.2 3,897,249 + BT3073 0.11 -1.6 3,897,249 + BT3073 0.11 +0.4 3,897,250 - BT3073 0.11 -1.6 3,897,252 - BT3073 0.12 -0.6 3,897,354 - BT3073 0.22 -3.7 3,897,359 - BT3073 0.22 +2.0 3,897,454 - BT3073 0.32 +0.4 3,897,541 - BT3073 0.41 -1.6 3,897,564 + BT3073 0.43 -1.6 3,897,584 - BT3073 0.45 -2.6 3,897,613 - BT3073 0.48 -0.7 3,897,669 - BT3073 0.54 -0.9 3,897,669 - BT3073 0.54 -1.6 3,897,733 + BT3073 0.60 -0.9 3,897,734 - BT3073 0.60 -2.1 3,897,734 - BT3073 0.60 -1.9 3,897,919 - BT3073 0.79 -1.1 3,898,042 + -1.1 3,898,134 - -1.6 3,898,138 - -1.6 3,898,188 - +0.4 3,898,248 - BT3074 0.12 -1.6 3,898,249 + BT3074 0.12 -1.1 3,898,250 - BT3074 0.12 +0.4 3,898,274 + BT3074 0.15 -1.6 3,898,323 + BT3074 0.20 -1.7 3,898,330 - BT3074 0.20 -2.8 3,898,450 + BT3074 0.33 -1.6 3,898,451 - BT3074 0.33 +0.4 3,898,577 + BT3074 0.46 +0.4 3,898,578 - BT3074 0.46 +0.4 3,898,581 + BT3074 0.46 +0.4 3,898,601 - BT3074 0.49 -2.1 3,898,639 - BT3074 0.52 +0.4 3,898,677 - BT3074 0.56 +0.4 3,898,743 + BT3074 0.63 -1.1 3,898,743 + BT3074 0.63 -1.1 3,898,764 + BT3074 0.65 -0.6 3,898,779 + BT3074 0.67 +0.4 3,899,025 - -0.6 3,899,036 - -0.6 3,899,083 + -1.6 3,899,084 - +0.4
Or see this region's nucleotide sequence