Experiment: Amylopectin from Potato; autoclaved 2.5 mg/ml (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2324 and BT2325 are separated by 38 nucleotides BT2325 and BT2326 overlap by 11 nucleotides BT2326 and BT2327 are separated by 279 nucleotides BT2327 and BT2328 are separated by 58 nucleotides
BT2324: BT2324 - DNA topoisomerase III (NCBI ptt file), at 2,900,707 to 2,902,806
BT2324
BT2325: BT2325 - hypothetical protein (NCBI ptt file), at 2,902,845 to 2,903,312
BT2325
BT2326: BT2326 - putative DNA methylase (NCBI ptt file), at 2,903,302 to 2,909,028
BT2326
BT2327: BT2327 - hypothetical protein (NCBI ptt file), at 2,909,308 to 2,909,772
BT2327
BT2328: BT2328 - conserved hypothetical protein (NCBI ptt file), at 2,909,831 to 2,910,562
BT2328
Position (kb)
2903
2904
2905
2906
2907
2908
2909
2910 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2902.332 kb on + strand, within BT2324 at 2902.400 kb on + strand, within BT2324 at 2902.442 kb on + strand, within BT2324 at 2902.450 kb on + strand, within BT2324 at 2902.451 kb on - strand, within BT2324 at 2902.451 kb on - strand, within BT2324 at 2902.451 kb on - strand, within BT2324 at 2902.732 kb on - strand at 2902.743 kb on + strand at 2902.743 kb on + strand at 2902.744 kb on - strand at 2902.749 kb on - strand at 2902.749 kb on - strand at 2902.805 kb on - strand at 2902.828 kb on - strand at 2902.843 kb on - strand at 2902.856 kb on + strand at 2902.900 kb on + strand, within BT2325 at 2902.905 kb on + strand, within BT2325 at 2902.905 kb on + strand, within BT2325 at 2902.906 kb on - strand, within BT2325 at 2902.906 kb on - strand, within BT2325 at 2902.906 kb on - strand, within BT2325 at 2903.284 kb on + strand at 2903.310 kb on + strand at 2903.313 kb on + strand at 2903.338 kb on - strand at 2903.338 kb on - strand at 2903.339 kb on + strand at 2903.339 kb on + strand at 2903.409 kb on - strand at 2903.477 kb on + strand at 2903.570 kb on - strand at 2903.921 kb on + strand, within BT2326 at 2903.922 kb on - strand, within BT2326 at 2903.922 kb on - strand, within BT2326 at 2903.990 kb on - strand, within BT2326 at 2904.225 kb on + strand, within BT2326 at 2904.226 kb on - strand, within BT2326 at 2904.301 kb on - strand, within BT2326 at 2904.319 kb on - strand, within BT2326 at 2904.998 kb on + strand, within BT2326 at 2904.998 kb on + strand, within BT2326 at 2905.003 kb on + strand, within BT2326 at 2905.094 kb on + strand, within BT2326 at 2905.105 kb on + strand, within BT2326 at 2905.105 kb on + strand, within BT2326 at 2905.118 kb on - strand, within BT2326 at 2905.265 kb on + strand, within BT2326 at 2905.265 kb on + strand, within BT2326 at 2905.357 kb on - strand, within BT2326 at 2905.421 kb on - strand, within BT2326 at 2905.486 kb on + strand, within BT2326 at 2905.487 kb on - strand, within BT2326 at 2905.507 kb on + strand, within BT2326 at 2905.771 kb on + strand, within BT2326 at 2905.908 kb on - strand, within BT2326 at 2906.053 kb on + strand, within BT2326 at 2906.152 kb on + strand, within BT2326 at 2907.201 kb on - strand, within BT2326 at 2907.407 kb on - strand, within BT2326 at 2907.407 kb on - strand, within BT2326 at 2907.414 kb on + strand, within BT2326 at 2907.430 kb on - strand, within BT2326 at 2907.545 kb on + strand, within BT2326 at 2907.546 kb on - strand, within BT2326 at 2907.805 kb on + strand, within BT2326 at 2907.832 kb on + strand, within BT2326 at 2907.833 kb on - strand, within BT2326 at 2907.833 kb on - strand, within BT2326 at 2907.850 kb on + strand, within BT2326 at 2907.941 kb on + strand, within BT2326 at 2907.942 kb on - strand, within BT2326 at 2907.956 kb on - strand, within BT2326 at 2908.161 kb on + strand, within BT2326 at 2908.408 kb on + strand, within BT2326 at 2908.414 kb on + strand, within BT2326 at 2908.504 kb on + strand at 2908.825 kb on + strand at 2908.826 kb on - strand at 2909.027 kb on - strand at 2909.098 kb on + strand at 2909.098 kb on + strand at 2909.099 kb on - strand at 2909.100 kb on + strand at 2909.101 kb on - strand at 2909.103 kb on + strand at 2909.105 kb on + strand at 2909.184 kb on + strand at 2909.193 kb on + strand at 2909.310 kb on - strand at 2909.310 kb on - strand at 2909.324 kb on - strand at 2909.324 kb on - strand at 2909.325 kb on + strand at 2909.326 kb on - strand at 2909.326 kb on - strand at 2909.328 kb on - strand at 2909.328 kb on - strand at 2909.343 kb on - strand at 2909.343 kb on - strand at 2909.343 kb on - strand at 2909.380 kb on + strand, within BT2327 at 2909.381 kb on - strand, within BT2327 at 2909.399 kb on - strand, within BT2327 at 2909.418 kb on + strand, within BT2327 at 2909.420 kb on + strand, within BT2327 at 2909.427 kb on + strand, within BT2327 at 2909.433 kb on + strand, within BT2327 at 2909.433 kb on + strand, within BT2327 at 2909.435 kb on + strand, within BT2327 at 2909.435 kb on + strand, within BT2327 at 2909.441 kb on + strand, within BT2327 at 2909.442 kb on - strand, within BT2327 at 2909.474 kb on + strand, within BT2327 at 2909.487 kb on + strand, within BT2327 at 2909.490 kb on + strand, within BT2327 at 2909.490 kb on + strand, within BT2327 at 2909.517 kb on + strand, within BT2327 at 2909.524 kb on + strand, within BT2327 at 2909.525 kb on - strand, within BT2327 at 2909.533 kb on + strand, within BT2327 at 2909.585 kb on + strand, within BT2327 at 2909.601 kb on + strand, within BT2327 at 2909.605 kb on - strand, within BT2327 at 2909.614 kb on + strand, within BT2327 at 2909.615 kb on - strand, within BT2327 at 2909.634 kb on - strand, within BT2327 at 2909.637 kb on - strand, within BT2327 at 2909.637 kb on - strand, within BT2327 at 2909.679 kb on + strand, within BT2327 at 2909.688 kb on - strand, within BT2327 at 2909.718 kb on + strand, within BT2327 at 2909.719 kb on - strand, within BT2327 at 2909.827 kb on + strand at 2909.839 kb on + strand at 2909.938 kb on + strand, within BT2328 at 2909.940 kb on + strand, within BT2328
Per-strain Table
Position Strand Gene LocusTag Fraction Amylopectin from Potato; autoclaved 2.5 mg/ml (C) remove 2,902,332 + BT2324 0.77 -0.9 2,902,400 + BT2324 0.81 +0.4 2,902,442 + BT2324 0.83 -0.8 2,902,450 + BT2324 0.83 +1.4 2,902,451 - BT2324 0.83 -0.9 2,902,451 - BT2324 0.83 +1.4 2,902,451 - BT2324 0.83 -1.9 2,902,732 - +0.4 2,902,743 + -0.6 2,902,743 + -1.1 2,902,744 - -1.0 2,902,749 - -1.6 2,902,749 - -0.9 2,902,805 - -1.3 2,902,828 - +0.4 2,902,843 - -1.6 2,902,856 + -0.9 2,902,900 + BT2325 0.12 -1.2 2,902,905 + BT2325 0.13 +0.0 2,902,905 + BT2325 0.13 -1.2 2,902,906 - BT2325 0.13 -1.3 2,902,906 - BT2325 0.13 -0.8 2,902,906 - BT2325 0.13 -0.6 2,903,284 + -1.3 2,903,310 + -0.7 2,903,313 + -0.8 2,903,338 - -0.6 2,903,338 - -1.6 2,903,339 + -0.8 2,903,339 + -1.2 2,903,409 - -0.6 2,903,477 + -2.2 2,903,570 - +0.4 2,903,921 + BT2326 0.11 -0.6 2,903,922 - BT2326 0.11 -0.4 2,903,922 - BT2326 0.11 -0.6 2,903,990 - BT2326 0.12 -1.3 2,904,225 + BT2326 0.16 -0.6 2,904,226 - BT2326 0.16 +0.0 2,904,301 - BT2326 0.17 -0.6 2,904,319 - BT2326 0.18 +1.2 2,904,998 + BT2326 0.30 -0.4 2,904,998 + BT2326 0.30 -0.5 2,905,003 + BT2326 0.30 -0.3 2,905,094 + BT2326 0.31 +0.0 2,905,105 + BT2326 0.31 -2.6 2,905,105 + BT2326 0.31 -1.6 2,905,118 - BT2326 0.32 -0.6 2,905,265 + BT2326 0.34 +1.2 2,905,265 + BT2326 0.34 +0.4 2,905,357 - BT2326 0.36 +0.0 2,905,421 - BT2326 0.37 -0.5 2,905,486 + BT2326 0.38 -1.0 2,905,487 - BT2326 0.38 -2.2 2,905,507 + BT2326 0.39 +0.4 2,905,771 + BT2326 0.43 -1.4 2,905,908 - BT2326 0.46 -1.9 2,906,053 + BT2326 0.48 -0.8 2,906,152 + BT2326 0.50 +0.2 2,907,201 - BT2326 0.68 +0.4 2,907,407 - BT2326 0.72 -0.6 2,907,407 - BT2326 0.72 -0.6 2,907,414 + BT2326 0.72 -0.6 2,907,430 - BT2326 0.72 -1.6 2,907,545 + BT2326 0.74 -0.2 2,907,546 - BT2326 0.74 -0.3 2,907,805 + BT2326 0.79 -0.4 2,907,832 + BT2326 0.79 +0.4 2,907,833 - BT2326 0.79 -0.2 2,907,833 - BT2326 0.79 -0.2 2,907,850 + BT2326 0.79 -1.2 2,907,941 + BT2326 0.81 +1.4 2,907,942 - BT2326 0.81 -3.6 2,907,956 - BT2326 0.81 -1.0 2,908,161 + BT2326 0.85 -1.1 2,908,408 + BT2326 0.89 -0.6 2,908,414 + BT2326 0.89 +0.4 2,908,504 + -0.6 2,908,825 + +1.4 2,908,826 - -2.9 2,909,027 - -1.0 2,909,098 + -0.4 2,909,098 + -0.6 2,909,099 - +0.4 2,909,100 + +0.4 2,909,101 - +0.0 2,909,103 + -1.2 2,909,105 + -0.7 2,909,184 + -1.0 2,909,193 + -1.2 2,909,310 - +0.4 2,909,310 - -2.0 2,909,324 - -1.3 2,909,324 - -1.6 2,909,325 + -0.6 2,909,326 - -0.2 2,909,326 - +0.4 2,909,328 - -1.2 2,909,328 - -1.6 2,909,343 - -0.9 2,909,343 - -0.9 2,909,343 - -1.1 2,909,380 + BT2327 0.15 -0.4 2,909,381 - BT2327 0.16 -2.6 2,909,399 - BT2327 0.20 -1.1 2,909,418 + BT2327 0.24 -1.5 2,909,420 + BT2327 0.24 -0.6 2,909,427 + BT2327 0.26 -1.2 2,909,433 + BT2327 0.27 -0.2 2,909,433 + BT2327 0.27 -0.6 2,909,435 + BT2327 0.27 -0.2 2,909,435 + BT2327 0.27 -0.9 2,909,441 + BT2327 0.29 -0.6 2,909,442 - BT2327 0.29 +0.4 2,909,474 + BT2327 0.36 -1.6 2,909,487 + BT2327 0.38 -0.3 2,909,490 + BT2327 0.39 -1.1 2,909,490 + BT2327 0.39 -1.7 2,909,517 + BT2327 0.45 -1.7 2,909,524 + BT2327 0.46 -2.2 2,909,525 - BT2327 0.47 +0.0 2,909,533 + BT2327 0.48 -0.5 2,909,585 + BT2327 0.60 -1.6 2,909,601 + BT2327 0.63 -0.3 2,909,605 - BT2327 0.64 -0.4 2,909,614 + BT2327 0.66 +0.0 2,909,615 - BT2327 0.66 -1.2 2,909,634 - BT2327 0.70 -0.8 2,909,637 - BT2327 0.71 -2.0 2,909,637 - BT2327 0.71 +0.4 2,909,679 + BT2327 0.80 -1.1 2,909,688 - BT2327 0.82 -0.3 2,909,718 + BT2327 0.88 +0.4 2,909,719 - BT2327 0.88 -1.9 2,909,827 + -1.4 2,909,839 + -2.4 2,909,938 + BT2328 0.15 -1.4 2,909,940 + BT2328 0.15 -0.6
Or see this region's nucleotide sequence