Experiment: Amylopectin from Potato; autoclaved 2.5 mg/ml (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2233 and BT2234 are separated by 54 nucleotides BT2234 and BT2235 overlap by 1 nucleotides BT2235 and BT2236 are separated by 67 nucleotides BT2236 and BT2237 are separated by 50 nucleotides BT2237 and BT2238 are separated by 1 nucleotides
BT2233: BT2233 - hypothetical protein (NCBI ptt file), at 2,802,745 to 2,803,044
BT2233
BT2234: BT2234 - putative cytosine/adenosine deaminase (NCBI ptt file), at 2,803,099 to 2,803,542
BT2234
BT2235: BT2235 - hypothetical protein (NCBI ptt file), at 2,803,542 to 2,803,775
BT2235
BT2236: BT2236 - conserved hypothetical protein (NCBI ptt file), at 2,803,843 to 2,804,208
BT2236
BT2237: BT2237 - conserved hypothetical protein (NCBI ptt file), at 2,804,259 to 2,804,615
BT2237
BT2238: BT2238 - putative biotin--(acetyl-CoA carboxylase) synthetase (NCBI ptt file), at 2,804,617 to 2,805,363
BT2238
Position (kb)
2803
2804
2805 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2803.045 kb on + strand at 2803.047 kb on + strand at 2803.052 kb on + strand at 2803.085 kb on + strand at 2803.641 kb on - strand, within BT2235 at 2803.733 kb on - strand, within BT2235 at 2803.776 kb on - strand at 2803.797 kb on - strand at 2804.013 kb on + strand, within BT2236 at 2804.099 kb on + strand, within BT2236 at 2804.168 kb on + strand, within BT2236 at 2804.177 kb on + strand at 2804.191 kb on + strand at 2804.201 kb on + strand at 2804.258 kb on + strand at 2804.290 kb on + strand at 2804.290 kb on + strand at 2804.332 kb on + strand, within BT2237 at 2804.445 kb on + strand, within BT2237 at 2804.515 kb on + strand, within BT2237
Per-strain Table
Position Strand Gene LocusTag Fraction Amylopectin from Potato; autoclaved 2.5 mg/ml (C) remove 2,803,045 + +0.4 2,803,047 + -0.8 2,803,052 + -1.6 2,803,085 + -1.5 2,803,641 - BT2235 0.42 -1.4 2,803,733 - BT2235 0.82 -0.8 2,803,776 - -0.6 2,803,797 - -1.1 2,804,013 + BT2236 0.46 -3.0 2,804,099 + BT2236 0.70 -2.4 2,804,168 + BT2236 0.89 +1.4 2,804,177 + +0.4 2,804,191 + +0.4 2,804,201 + -0.6 2,804,258 + -2.1 2,804,290 + -1.1 2,804,290 + -0.6 2,804,332 + BT2237 0.20 +0.1 2,804,445 + BT2237 0.52 -2.1 2,804,515 + BT2237 0.72 -0.6
Or see this region's nucleotide sequence