Experiment: Amylopectin from Potato; autoclaved 2.5 mg/ml (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1842 and BT1843 are separated by 117 nucleotides BT1843 and BT1844 overlap by 4 nucleotides BT1844 and BT1845 are separated by 160 nucleotides
BT1842: BT1842 - alpha-L-fucosidase precursor (NCBI ptt file), at 2,313,348 to 2,314,664
BT1842
BT1843: BT1843 - adenylosuccinate synthetase (NCBI ptt file), at 2,314,782 to 2,316,053
BT1843
BT1844: BT1844 - transcriptional regulator, putative iron uptake regulation protein (NCBI ptt file), at 2,316,050 to 2,316,541
BT1844
BT1845: BT1845 - conserved hypothetical protein (NCBI ptt file), at 2,316,702 to 2,317,367
BT1845
Position (kb)
2314
2315
2316
2317 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2313.783 kb on - strand, within BT1842 at 2313.788 kb on + strand, within BT1842 at 2313.788 kb on + strand, within BT1842 at 2313.791 kb on - strand, within BT1842 at 2313.800 kb on + strand, within BT1842 at 2313.800 kb on + strand, within BT1842 at 2313.866 kb on + strand, within BT1842 at 2313.895 kb on - strand, within BT1842 at 2313.936 kb on - strand, within BT1842 at 2313.943 kb on - strand, within BT1842 at 2313.960 kb on + strand, within BT1842 at 2313.979 kb on + strand, within BT1842 at 2313.979 kb on + strand, within BT1842 at 2314.067 kb on + strand, within BT1842 at 2314.108 kb on + strand, within BT1842 at 2314.201 kb on + strand, within BT1842 at 2314.204 kb on + strand, within BT1842 at 2314.304 kb on - strand, within BT1842 at 2314.357 kb on + strand at 2314.361 kb on + strand, within BT1842 at 2314.361 kb on + strand, within BT1842 at 2314.395 kb on - strand, within BT1842 at 2314.441 kb on + strand, within BT1842 at 2314.463 kb on + strand, within BT1842 at 2314.501 kb on + strand, within BT1842 at 2314.532 kb on + strand, within BT1842 at 2314.629 kb on + strand at 2314.629 kb on + strand at 2314.632 kb on + strand at 2314.633 kb on - strand at 2314.636 kb on - strand at 2314.636 kb on - strand at 2314.636 kb on - strand at 2314.675 kb on - strand at 2314.678 kb on + strand at 2314.740 kb on - strand at 2314.745 kb on - strand at 2314.745 kb on - strand at 2314.746 kb on + strand at 2314.762 kb on + strand at 2314.793 kb on + strand at 2314.796 kb on - strand at 2314.796 kb on - strand at 2314.879 kb on + strand at 2314.879 kb on + strand at 2314.929 kb on - strand, within BT1843 at 2314.957 kb on + strand, within BT1843 at 2314.957 kb on + strand, within BT1843 at 2314.959 kb on + strand, within BT1843 at 2314.959 kb on + strand, within BT1843 at 2314.960 kb on - strand, within BT1843 at 2315.017 kb on - strand, within BT1843 at 2315.023 kb on + strand, within BT1843 at 2315.023 kb on + strand, within BT1843 at 2315.045 kb on + strand, within BT1843 at 2315.101 kb on - strand, within BT1843 at 2315.101 kb on - strand, within BT1843 at 2315.189 kb on + strand, within BT1843 at 2315.245 kb on - strand, within BT1843 at 2315.302 kb on - strand, within BT1843 at 2315.349 kb on - strand, within BT1843 at 2315.486 kb on + strand, within BT1843 at 2315.524 kb on + strand, within BT1843 at 2315.649 kb on - strand, within BT1843 at 2315.705 kb on - strand, within BT1843 at 2315.901 kb on + strand, within BT1843 at 2315.901 kb on + strand, within BT1843 at 2315.901 kb on + strand, within BT1843 at 2315.905 kb on + strand, within BT1843 at 2315.930 kb on + strand at 2315.930 kb on + strand at 2315.930 kb on + strand at 2315.931 kb on - strand at 2315.931 kb on - strand at 2315.931 kb on - strand at 2315.974 kb on + strand at 2315.985 kb on + strand at 2315.989 kb on - strand at 2316.034 kb on - strand at 2316.046 kb on - strand at 2316.092 kb on - strand at 2316.127 kb on + strand, within BT1844 at 2316.128 kb on - strand, within BT1844 at 2316.130 kb on - strand, within BT1844 at 2316.229 kb on + strand, within BT1844 at 2316.233 kb on + strand, within BT1844 at 2316.233 kb on + strand at 2316.233 kb on + strand, within BT1844 at 2316.274 kb on - strand, within BT1844 at 2316.337 kb on + strand, within BT1844 at 2316.341 kb on - strand, within BT1844 at 2316.341 kb on - strand, within BT1844 at 2316.394 kb on - strand, within BT1844 at 2316.400 kb on + strand, within BT1844 at 2316.429 kb on + strand, within BT1844 at 2316.429 kb on + strand, within BT1844 at 2316.490 kb on - strand, within BT1844 at 2316.514 kb on + strand at 2316.568 kb on + strand at 2316.607 kb on + strand at 2316.612 kb on - strand at 2316.618 kb on + strand at 2316.619 kb on - strand at 2316.625 kb on + strand at 2316.628 kb on + strand at 2316.632 kb on + strand at 2316.633 kb on - strand at 2316.642 kb on - strand at 2316.642 kb on - strand at 2316.645 kb on + strand at 2316.682 kb on + strand at 2316.683 kb on - strand at 2316.683 kb on - strand at 2316.683 kb on - strand at 2316.687 kb on - strand at 2316.693 kb on + strand at 2316.697 kb on - strand at 2316.697 kb on - strand at 2316.700 kb on + strand at 2316.825 kb on - strand, within BT1845 at 2316.825 kb on - strand, within BT1845 at 2316.825 kb on - strand, within BT1845 at 2316.826 kb on + strand, within BT1845 at 2316.827 kb on - strand, within BT1845
Per-strain Table
Position Strand Gene LocusTag Fraction Amylopectin from Potato; autoclaved 2.5 mg/ml (C) remove 2,313,783 - BT1842 0.33 -0.6 2,313,788 + BT1842 0.33 -0.6 2,313,788 + BT1842 0.33 +0.0 2,313,791 - BT1842 0.34 -0.6 2,313,800 + BT1842 0.34 -1.6 2,313,800 + BT1842 0.34 +1.4 2,313,866 + BT1842 0.39 -1.2 2,313,895 - BT1842 0.42 -2.4 2,313,936 - BT1842 0.45 -1.3 2,313,943 - BT1842 0.45 -1.4 2,313,960 + BT1842 0.46 -0.6 2,313,979 + BT1842 0.48 -0.6 2,313,979 + BT1842 0.48 -0.4 2,314,067 + BT1842 0.55 -0.2 2,314,108 + BT1842 0.58 -0.6 2,314,201 + BT1842 0.65 -1.6 2,314,204 + BT1842 0.65 +1.1 2,314,304 - BT1842 0.73 -0.6 2,314,357 + +0.4 2,314,361 + BT1842 0.77 -0.6 2,314,361 + BT1842 0.77 -0.6 2,314,395 - BT1842 0.79 -1.6 2,314,441 + BT1842 0.83 -0.5 2,314,463 + BT1842 0.85 -0.2 2,314,501 + BT1842 0.88 -1.2 2,314,532 + BT1842 0.90 -2.1 2,314,629 + -2.4 2,314,629 + +1.3 2,314,632 + -1.0 2,314,633 - -1.6 2,314,636 - -0.2 2,314,636 - -4.9 2,314,636 - -0.7 2,314,675 - -0.6 2,314,678 + -0.6 2,314,740 - -0.1 2,314,745 - +1.4 2,314,745 - +0.2 2,314,746 + -1.2 2,314,762 + -0.2 2,314,793 + +0.4 2,314,796 - -2.2 2,314,796 - -1.1 2,314,879 + -0.9 2,314,879 + -3.5 2,314,929 - BT1843 0.12 -1.6 2,314,957 + BT1843 0.14 +0.4 2,314,957 + BT1843 0.14 -2.4 2,314,959 + BT1843 0.14 -3.2 2,314,959 + BT1843 0.14 -1.2 2,314,960 - BT1843 0.14 -3.9 2,315,017 - BT1843 0.18 +0.4 2,315,023 + BT1843 0.19 +0.4 2,315,023 + BT1843 0.19 -0.6 2,315,045 + BT1843 0.21 -0.6 2,315,101 - BT1843 0.25 -0.6 2,315,101 - BT1843 0.25 +0.4 2,315,189 + BT1843 0.32 -2.2 2,315,245 - BT1843 0.36 -2.7 2,315,302 - BT1843 0.41 -1.6 2,315,349 - BT1843 0.45 -2.6 2,315,486 + BT1843 0.55 -4.4 2,315,524 + BT1843 0.58 -4.1 2,315,649 - BT1843 0.68 -2.2 2,315,705 - BT1843 0.73 -4.1 2,315,901 + BT1843 0.88 -3.2 2,315,901 + BT1843 0.88 -2.4 2,315,901 + BT1843 0.88 +0.4 2,315,905 + BT1843 0.88 -3.8 2,315,930 + -0.6 2,315,930 + -1.6 2,315,930 + -0.6 2,315,931 - -1.6 2,315,931 - -4.2 2,315,931 - -2.9 2,315,974 + -0.6 2,315,985 + -3.7 2,315,989 - -2.2 2,316,034 - -1.2 2,316,046 - -3.8 2,316,092 - -1.9 2,316,127 + BT1844 0.16 +0.4 2,316,128 - BT1844 0.16 -1.6 2,316,130 - BT1844 0.16 -0.4 2,316,229 + BT1844 0.36 -2.9 2,316,233 + BT1844 0.37 -3.5 2,316,233 + -0.6 2,316,233 + BT1844 0.37 -1.2 2,316,274 - BT1844 0.46 -2.4 2,316,337 + BT1844 0.58 -2.9 2,316,341 - BT1844 0.59 -2.2 2,316,341 - BT1844 0.59 -0.0 2,316,394 - BT1844 0.70 -0.2 2,316,400 + BT1844 0.71 -1.2 2,316,429 + BT1844 0.77 -3.8 2,316,429 + BT1844 0.77 -4.2 2,316,490 - BT1844 0.89 +0.4 2,316,514 + -0.6 2,316,568 + -3.7 2,316,607 + -2.6 2,316,612 - -0.6 2,316,618 + -1.1 2,316,619 - -2.2 2,316,625 + -0.9 2,316,628 + -1.2 2,316,632 + -1.8 2,316,633 - -1.2 2,316,642 - -0.6 2,316,642 - -1.6 2,316,645 + -1.2 2,316,682 + -0.7 2,316,683 - -0.9 2,316,683 - -2.8 2,316,683 - -0.8 2,316,687 - -2.0 2,316,693 + -0.7 2,316,697 - -1.2 2,316,697 - -2.4 2,316,700 + -1.2 2,316,825 - BT1845 0.18 +0.4 2,316,825 - BT1845 0.18 -0.2 2,316,825 - BT1845 0.18 -0.8 2,316,826 + BT1845 0.19 +0.4 2,316,827 - BT1845 0.19 -0.6
Or see this region's nucleotide sequence