Experiment: Amylopectin from Potato; autoclaved 2.5 mg/ml (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1418 and BT1419 are separated by 156 nucleotides BT1419 and BT1420 overlap by 1 nucleotides BT1420 and BT1421 are separated by 86 nucleotides BT1421 and BT1422 are separated by 196 nucleotides BT1422 and BT1423 are separated by 2 nucleotides
BT1418: BT1418 - quinol oxidase (NCBI ptt file), at 1,751,850 to 1,752,446
BT1418
BT1419: BT1419 - hypothetical protein (NCBI ptt file), at 1,752,603 to 1,753,097
BT1419
BT1420: BT1420 - conserved hypothetical protein (NCBI ptt file), at 1,753,097 to 1,755,253
BT1420
BT1421: BT1421 - hypothetical protein (NCBI ptt file), at 1,755,340 to 1,755,654
BT1421
BT1422: BT1422 - conserved hypothetical protein (NCBI ptt file), at 1,755,851 to 1,756,195
BT1422
BT1423: BT1423 - conserved hypothetical protein (NCBI ptt file), at 1,756,198 to 1,756,584
BT1423
Position (kb)
1753
1754
1755
1756 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1752.323 kb on + strand, within BT1418 at 1752.323 kb on + strand, within BT1418 at 1752.324 kb on - strand, within BT1418 at 1752.340 kb on + strand, within BT1418 at 1752.345 kb on - strand, within BT1418 at 1752.368 kb on + strand, within BT1418 at 1752.368 kb on + strand, within BT1418 at 1752.393 kb on + strand at 1752.429 kb on - strand at 1752.472 kb on + strand at 1752.497 kb on + strand at 1752.497 kb on + strand at 1752.508 kb on - strand at 1752.600 kb on + strand at 1752.667 kb on + strand, within BT1419 at 1752.747 kb on - strand, within BT1419 at 1752.791 kb on - strand, within BT1419 at 1752.879 kb on + strand, within BT1419 at 1752.879 kb on + strand, within BT1419 at 1752.893 kb on - strand, within BT1419 at 1753.037 kb on + strand, within BT1419 at 1753.191 kb on + strand at 1753.192 kb on - strand at 1753.303 kb on + strand at 1753.369 kb on - strand, within BT1420 at 1753.393 kb on + strand, within BT1420 at 1753.501 kb on + strand, within BT1420 at 1753.665 kb on + strand, within BT1420 at 1753.799 kb on - strand, within BT1420 at 1753.906 kb on + strand, within BT1420 at 1753.916 kb on + strand, within BT1420 at 1753.932 kb on + strand, within BT1420 at 1753.990 kb on + strand, within BT1420 at 1753.999 kb on - strand, within BT1420 at 1754.004 kb on + strand, within BT1420 at 1754.045 kb on - strand, within BT1420 at 1754.054 kb on - strand, within BT1420 at 1754.153 kb on - strand, within BT1420 at 1754.512 kb on + strand, within BT1420 at 1754.513 kb on - strand, within BT1420 at 1754.784 kb on - strand, within BT1420 at 1754.798 kb on + strand, within BT1420 at 1754.798 kb on + strand, within BT1420 at 1754.948 kb on + strand, within BT1420 at 1755.013 kb on + strand, within BT1420 at 1755.013 kb on + strand, within BT1420 at 1755.169 kb on + strand at 1755.170 kb on - strand at 1755.170 kb on - strand at 1755.212 kb on + strand at 1755.238 kb on + strand at 1755.272 kb on + strand at 1755.301 kb on + strand at 1755.302 kb on - strand at 1755.337 kb on + strand at 1755.341 kb on + strand at 1755.408 kb on + strand, within BT1421 at 1755.408 kb on + strand, within BT1421 at 1755.485 kb on + strand, within BT1421 at 1755.610 kb on + strand, within BT1421 at 1755.623 kb on - strand at 1755.626 kb on + strand at 1755.626 kb on + strand at 1755.650 kb on + strand at 1755.851 kb on - strand at 1755.870 kb on + strand at 1755.871 kb on - strand at 1755.933 kb on + strand, within BT1422 at 1755.966 kb on + strand, within BT1422 at 1756.021 kb on - strand, within BT1422 at 1756.045 kb on - strand, within BT1422 at 1756.125 kb on + strand, within BT1422 at 1756.126 kb on - strand, within BT1422 at 1756.138 kb on + strand, within BT1422 at 1756.138 kb on + strand, within BT1422 at 1756.182 kb on + strand at 1756.182 kb on + strand at 1756.185 kb on + strand at 1756.190 kb on - strand at 1756.220 kb on + strand at 1756.221 kb on - strand at 1756.221 kb on - strand at 1756.221 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Amylopectin from Potato; autoclaved 2.5 mg/ml (C) remove 1,752,323 + BT1418 0.79 -1.2 1,752,323 + BT1418 0.79 -1.2 1,752,324 - BT1418 0.79 +0.0 1,752,340 + BT1418 0.82 -1.7 1,752,345 - BT1418 0.83 -0.2 1,752,368 + BT1418 0.87 -1.3 1,752,368 + BT1418 0.87 +0.6 1,752,393 + -0.5 1,752,429 - +0.3 1,752,472 + -1.4 1,752,497 + -0.7 1,752,497 + +0.3 1,752,508 - +0.9 1,752,600 + -3.0 1,752,667 + BT1419 0.13 -2.8 1,752,747 - BT1419 0.29 +0.4 1,752,791 - BT1419 0.38 -1.6 1,752,879 + BT1419 0.56 -0.1 1,752,879 + BT1419 0.56 -0.6 1,752,893 - BT1419 0.59 -1.2 1,753,037 + BT1419 0.88 -2.5 1,753,191 + -2.6 1,753,192 - +0.4 1,753,303 + -1.0 1,753,369 - BT1420 0.13 +0.4 1,753,393 + BT1420 0.14 -2.0 1,753,501 + BT1420 0.19 -0.6 1,753,665 + BT1420 0.26 -3.2 1,753,799 - BT1420 0.33 -2.2 1,753,906 + BT1420 0.38 -0.7 1,753,916 + BT1420 0.38 -2.5 1,753,932 + BT1420 0.39 -2.0 1,753,990 + BT1420 0.41 -2.0 1,753,999 - BT1420 0.42 -2.5 1,754,004 + BT1420 0.42 +0.3 1,754,045 - BT1420 0.44 +0.3 1,754,054 - BT1420 0.44 +0.3 1,754,153 - BT1420 0.49 -1.7 1,754,512 + BT1420 0.66 +0.3 1,754,513 - BT1420 0.66 -1.2 1,754,784 - BT1420 0.78 +0.3 1,754,798 + BT1420 0.79 -1.2 1,754,798 + BT1420 0.79 -1.2 1,754,948 + BT1420 0.86 -3.1 1,755,013 + BT1420 0.89 -1.2 1,755,013 + BT1420 0.89 -2.4 1,755,169 + -0.6 1,755,170 - +0.4 1,755,170 - -0.6 1,755,212 + +1.4 1,755,238 + -1.2 1,755,272 + -0.6 1,755,301 + -1.2 1,755,302 - +0.9 1,755,337 + -1.6 1,755,341 + +0.4 1,755,408 + BT1421 0.22 -2.2 1,755,408 + BT1421 0.22 -1.6 1,755,485 + BT1421 0.46 -3.0 1,755,610 + BT1421 0.86 -0.4 1,755,623 - -1.4 1,755,626 + -1.6 1,755,626 + -0.6 1,755,650 + +0.4 1,755,851 - -1.1 1,755,870 + -1.6 1,755,871 - -0.6 1,755,933 + BT1422 0.24 +0.4 1,755,966 + BT1422 0.33 -2.1 1,756,021 - BT1422 0.49 -2.0 1,756,045 - BT1422 0.56 -2.2 1,756,125 + BT1422 0.79 -1.2 1,756,126 - BT1422 0.80 -0.2 1,756,138 + BT1422 0.83 -2.6 1,756,138 + BT1422 0.83 -1.6 1,756,182 + -0.4 1,756,182 + -0.6 1,756,185 + -1.2 1,756,190 - -2.2 1,756,220 + -0.6 1,756,221 - -1.5 1,756,221 - +0.4 1,756,221 - -1.6
Or see this region's nucleotide sequence