Experiment: Amylopectin from Potato; autoclaved 2.5 mg/ml (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1273 and BT1274 are separated by 109 nucleotides BT1274 and BT1275 are separated by 15 nucleotides BT1275 and BT1276 are separated by 7 nucleotides BT1276 and BT1277 are separated by 17 nucleotides
BT1273: BT1273 - L-fucose isomerase (NCBI ptt file), at 1,582,840 to 1,584,615
BT1273
BT1274: BT1274 - L-fuculose-1-phosphate aldolase FucA (4.1.2.17); D-ribulose-1-phosphate aldolase (from data) , at 1,584,725 to 1,585,363
BT1274
BT1275: BT1275 - L-fuculose kinase fucK (EC 2.7.1.51); D-ribulose kinase (EC 2.7.1.47) (from data) , at 1,585,379 to 1,586,809
BT1275
BT1276: BT1276 - L-fucose mutarotase FucU (EC 5.1.3.29) (from data) , at 1,586,817 to 1,587,209
BT1276
BT1277: BT1277 - L-fucose/D-arabinose symporter FucP (from data) , at 1,587,227 to 1,588,543
BT1277
Position (kb)
1585
1586
1587 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1584.394 kb on + strand, within BT1273 at 1584.439 kb on + strand at 1584.439 kb on + strand at 1584.509 kb on + strand at 1584.509 kb on + strand at 1584.510 kb on - strand at 1584.588 kb on + strand at 1584.604 kb on - strand at 1584.666 kb on + strand at 1584.699 kb on + strand at 1584.700 kb on - strand at 1584.712 kb on - strand at 1584.725 kb on - strand at 1584.899 kb on - strand, within BT1274 at 1584.902 kb on - strand, within BT1274 at 1584.941 kb on - strand, within BT1274 at 1584.987 kb on + strand, within BT1274 at 1584.988 kb on - strand, within BT1274 at 1584.993 kb on + strand, within BT1274 at 1585.108 kb on - strand, within BT1274 at 1585.210 kb on - strand, within BT1274 at 1585.236 kb on - strand, within BT1274 at 1585.246 kb on + strand, within BT1274 at 1585.275 kb on + strand, within BT1274 at 1585.361 kb on + strand at 1585.376 kb on - strand at 1585.403 kb on + strand at 1585.486 kb on - strand at 1585.558 kb on + strand, within BT1275 at 1585.617 kb on + strand, within BT1275 at 1585.618 kb on - strand, within BT1275 at 1585.622 kb on - strand, within BT1275 at 1585.723 kb on - strand, within BT1275 at 1585.731 kb on + strand, within BT1275 at 1585.751 kb on + strand, within BT1275 at 1585.751 kb on + strand, within BT1275 at 1585.752 kb on - strand, within BT1275 at 1585.768 kb on - strand, within BT1275 at 1585.768 kb on - strand, within BT1275 at 1585.768 kb on - strand, within BT1275 at 1585.773 kb on + strand, within BT1275 at 1585.773 kb on + strand, within BT1275 at 1585.774 kb on - strand, within BT1275 at 1585.787 kb on - strand, within BT1275 at 1585.828 kb on - strand, within BT1275 at 1585.877 kb on + strand, within BT1275 at 1585.881 kb on - strand, within BT1275 at 1586.070 kb on + strand, within BT1275 at 1586.208 kb on + strand, within BT1275 at 1586.257 kb on - strand, within BT1275 at 1586.352 kb on - strand, within BT1275 at 1586.357 kb on - strand, within BT1275 at 1586.399 kb on - strand, within BT1275 at 1586.520 kb on + strand, within BT1275 at 1586.521 kb on - strand, within BT1275 at 1586.764 kb on - strand at 1586.767 kb on - strand at 1586.790 kb on + strand at 1586.791 kb on - strand at 1586.791 kb on - strand at 1586.915 kb on - strand, within BT1276 at 1586.950 kb on + strand, within BT1276 at 1586.951 kb on - strand, within BT1276 at 1586.951 kb on - strand, within BT1276 at 1586.951 kb on - strand, within BT1276 at 1586.983 kb on + strand, within BT1276 at 1586.983 kb on + strand, within BT1276 at 1587.006 kb on + strand, within BT1276 at 1587.006 kb on + strand, within BT1276 at 1587.196 kb on + strand at 1587.200 kb on + strand at 1587.238 kb on + strand at 1587.238 kb on + strand at 1587.239 kb on - strand at 1587.248 kb on - strand at 1587.277 kb on + strand at 1587.289 kb on - strand at 1587.292 kb on + strand at 1587.292 kb on + strand at 1587.293 kb on - strand at 1587.296 kb on - strand at 1587.334 kb on + strand at 1587.484 kb on - strand, within BT1277 at 1587.504 kb on - strand, within BT1277 at 1587.551 kb on + strand, within BT1277 at 1587.572 kb on + strand, within BT1277 at 1587.625 kb on + strand, within BT1277 at 1587.628 kb on - strand, within BT1277 at 1587.655 kb on + strand, within BT1277 at 1587.717 kb on + strand, within BT1277 at 1587.718 kb on - strand, within BT1277 at 1587.740 kb on - strand, within BT1277
Per-strain Table
Position Strand Gene LocusTag Fraction Amylopectin from Potato; autoclaved 2.5 mg/ml (C) remove 1,584,394 + BT1273 0.88 -0.7 1,584,439 + +0.9 1,584,439 + -1.2 1,584,509 + -1.4 1,584,509 + +1.3 1,584,510 - -0.7 1,584,588 + +0.9 1,584,604 - -0.7 1,584,666 + +0.3 1,584,699 + -1.6 1,584,700 - +1.3 1,584,712 - -1.2 1,584,725 - -1.2 1,584,899 - BT1274 0.27 +0.3 1,584,902 - BT1274 0.28 -0.8 1,584,941 - BT1274 0.34 -1.2 1,584,987 + BT1274 0.41 +1.3 1,584,988 - BT1274 0.41 -0.3 1,584,993 + BT1274 0.42 +1.1 1,585,108 - BT1274 0.60 -0.7 1,585,210 - BT1274 0.76 -0.7 1,585,236 - BT1274 0.80 -0.7 1,585,246 + BT1274 0.82 -2.2 1,585,275 + BT1274 0.86 +0.3 1,585,361 + -1.7 1,585,376 - -0.4 1,585,403 + +0.1 1,585,486 - -0.2 1,585,558 + BT1275 0.13 -0.7 1,585,617 + BT1275 0.17 +0.8 1,585,618 - BT1275 0.17 -0.4 1,585,622 - BT1275 0.17 -1.2 1,585,723 - BT1275 0.24 -0.1 1,585,731 + BT1275 0.25 -2.0 1,585,751 + BT1275 0.26 -0.7 1,585,751 + BT1275 0.26 -1.9 1,585,752 - BT1275 0.26 -2.3 1,585,768 - BT1275 0.27 -1.2 1,585,768 - BT1275 0.27 -0.9 1,585,768 - BT1275 0.27 +0.3 1,585,773 + BT1275 0.28 -0.2 1,585,773 + BT1275 0.28 +1.7 1,585,774 - BT1275 0.28 -2.0 1,585,787 - BT1275 0.29 +0.3 1,585,828 - BT1275 0.31 -1.2 1,585,877 + BT1275 0.35 -1.2 1,585,881 - BT1275 0.35 -0.7 1,586,070 + BT1275 0.48 -0.7 1,586,208 + BT1275 0.58 -2.2 1,586,257 - BT1275 0.61 -1.7 1,586,352 - BT1275 0.68 +2.3 1,586,357 - BT1275 0.68 -1.4 1,586,399 - BT1275 0.71 +0.3 1,586,520 + BT1275 0.80 +0.3 1,586,521 - BT1275 0.80 -1.0 1,586,764 - -1.2 1,586,767 - -1.2 1,586,790 + -0.5 1,586,791 - -0.7 1,586,791 - +0.3 1,586,915 - BT1276 0.25 +0.9 1,586,950 + BT1276 0.34 -0.4 1,586,951 - BT1276 0.34 +0.3 1,586,951 - BT1276 0.34 +0.3 1,586,951 - BT1276 0.34 -1.2 1,586,983 + BT1276 0.42 -1.7 1,586,983 + BT1276 0.42 -2.0 1,587,006 + BT1276 0.48 +0.0 1,587,006 + BT1276 0.48 +0.3 1,587,196 + +1.3 1,587,200 + +0.3 1,587,238 + -0.2 1,587,238 + -0.7 1,587,239 - +0.5 1,587,248 - -3.4 1,587,277 + -1.2 1,587,289 - +1.3 1,587,292 + -1.2 1,587,292 + +0.3 1,587,293 - -1.2 1,587,296 - -2.2 1,587,334 + -0.7 1,587,484 - BT1277 0.20 -1.2 1,587,504 - BT1277 0.21 -2.2 1,587,551 + BT1277 0.25 -1.8 1,587,572 + BT1277 0.26 -1.0 1,587,625 + BT1277 0.30 -2.2 1,587,628 - BT1277 0.30 -2.0 1,587,655 + BT1277 0.32 +0.3 1,587,717 + BT1277 0.37 -0.7 1,587,718 - BT1277 0.37 -1.1 1,587,740 - BT1277 0.39 -1.8
Or see this region's nucleotide sequence