Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT1275

Experiment: Amylopectin from Potato; autoclaved 2.5 mg/ml (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT1273 and BT1274 are separated by 109 nucleotidesBT1274 and BT1275 are separated by 15 nucleotidesBT1275 and BT1276 are separated by 7 nucleotidesBT1276 and BT1277 are separated by 17 nucleotides BT1273: BT1273 - L-fucose isomerase (NCBI ptt file), at 1,582,840 to 1,584,615 BT1273 BT1274: BT1274 - L-fuculose-1-phosphate aldolase FucA (4.1.2.17); D-ribulose-1-phosphate aldolase (from data), at 1,584,725 to 1,585,363 BT1274 BT1275: BT1275 - L-fuculose kinase fucK (EC 2.7.1.51); D-ribulose kinase (EC 2.7.1.47) (from data), at 1,585,379 to 1,586,809 BT1275 BT1276: BT1276 - L-fucose mutarotase FucU (EC 5.1.3.29) (from data), at 1,586,817 to 1,587,209 BT1276 BT1277: BT1277 - L-fucose/D-arabinose symporter FucP (from data), at 1,587,227 to 1,588,543 BT1277 Position (kb) 1585 1586 1587Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1584.394 kb on + strand, within BT1273at 1584.439 kb on + strandat 1584.439 kb on + strandat 1584.509 kb on + strandat 1584.509 kb on + strandat 1584.510 kb on - strandat 1584.588 kb on + strandat 1584.604 kb on - strandat 1584.666 kb on + strandat 1584.699 kb on + strandat 1584.700 kb on - strandat 1584.712 kb on - strandat 1584.725 kb on - strandat 1584.899 kb on - strand, within BT1274at 1584.902 kb on - strand, within BT1274at 1584.941 kb on - strand, within BT1274at 1584.987 kb on + strand, within BT1274at 1584.988 kb on - strand, within BT1274at 1584.993 kb on + strand, within BT1274at 1585.108 kb on - strand, within BT1274at 1585.210 kb on - strand, within BT1274at 1585.236 kb on - strand, within BT1274at 1585.246 kb on + strand, within BT1274at 1585.275 kb on + strand, within BT1274at 1585.361 kb on + strandat 1585.376 kb on - strandat 1585.403 kb on + strandat 1585.486 kb on - strandat 1585.558 kb on + strand, within BT1275at 1585.617 kb on + strand, within BT1275at 1585.618 kb on - strand, within BT1275at 1585.622 kb on - strand, within BT1275at 1585.723 kb on - strand, within BT1275at 1585.731 kb on + strand, within BT1275at 1585.751 kb on + strand, within BT1275at 1585.751 kb on + strand, within BT1275at 1585.752 kb on - strand, within BT1275at 1585.768 kb on - strand, within BT1275at 1585.768 kb on - strand, within BT1275at 1585.768 kb on - strand, within BT1275at 1585.773 kb on + strand, within BT1275at 1585.773 kb on + strand, within BT1275at 1585.774 kb on - strand, within BT1275at 1585.787 kb on - strand, within BT1275at 1585.828 kb on - strand, within BT1275at 1585.877 kb on + strand, within BT1275at 1585.881 kb on - strand, within BT1275at 1586.070 kb on + strand, within BT1275at 1586.208 kb on + strand, within BT1275at 1586.257 kb on - strand, within BT1275at 1586.352 kb on - strand, within BT1275at 1586.357 kb on - strand, within BT1275at 1586.399 kb on - strand, within BT1275at 1586.520 kb on + strand, within BT1275at 1586.521 kb on - strand, within BT1275at 1586.764 kb on - strandat 1586.767 kb on - strandat 1586.790 kb on + strandat 1586.791 kb on - strandat 1586.791 kb on - strandat 1586.915 kb on - strand, within BT1276at 1586.950 kb on + strand, within BT1276at 1586.951 kb on - strand, within BT1276at 1586.951 kb on - strand, within BT1276at 1586.951 kb on - strand, within BT1276at 1586.983 kb on + strand, within BT1276at 1586.983 kb on + strand, within BT1276at 1587.006 kb on + strand, within BT1276at 1587.006 kb on + strand, within BT1276at 1587.196 kb on + strandat 1587.200 kb on + strandat 1587.238 kb on + strandat 1587.238 kb on + strandat 1587.239 kb on - strandat 1587.248 kb on - strandat 1587.277 kb on + strandat 1587.289 kb on - strandat 1587.292 kb on + strandat 1587.292 kb on + strandat 1587.293 kb on - strandat 1587.296 kb on - strandat 1587.334 kb on + strandat 1587.484 kb on - strand, within BT1277at 1587.504 kb on - strand, within BT1277at 1587.551 kb on + strand, within BT1277at 1587.572 kb on + strand, within BT1277at 1587.625 kb on + strand, within BT1277at 1587.628 kb on - strand, within BT1277at 1587.655 kb on + strand, within BT1277at 1587.717 kb on + strand, within BT1277at 1587.718 kb on - strand, within BT1277at 1587.740 kb on - strand, within BT1277

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Per-strain Table

Position Strand Gene LocusTag Fraction Amylopectin from Potato; autoclaved 2.5 mg/ml (C)
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1,584,394 + BT1273 0.88 -0.7
1,584,439 + +0.9
1,584,439 + -1.2
1,584,509 + -1.4
1,584,509 + +1.3
1,584,510 - -0.7
1,584,588 + +0.9
1,584,604 - -0.7
1,584,666 + +0.3
1,584,699 + -1.6
1,584,700 - +1.3
1,584,712 - -1.2
1,584,725 - -1.2
1,584,899 - BT1274 0.27 +0.3
1,584,902 - BT1274 0.28 -0.8
1,584,941 - BT1274 0.34 -1.2
1,584,987 + BT1274 0.41 +1.3
1,584,988 - BT1274 0.41 -0.3
1,584,993 + BT1274 0.42 +1.1
1,585,108 - BT1274 0.60 -0.7
1,585,210 - BT1274 0.76 -0.7
1,585,236 - BT1274 0.80 -0.7
1,585,246 + BT1274 0.82 -2.2
1,585,275 + BT1274 0.86 +0.3
1,585,361 + -1.7
1,585,376 - -0.4
1,585,403 + +0.1
1,585,486 - -0.2
1,585,558 + BT1275 0.13 -0.7
1,585,617 + BT1275 0.17 +0.8
1,585,618 - BT1275 0.17 -0.4
1,585,622 - BT1275 0.17 -1.2
1,585,723 - BT1275 0.24 -0.1
1,585,731 + BT1275 0.25 -2.0
1,585,751 + BT1275 0.26 -0.7
1,585,751 + BT1275 0.26 -1.9
1,585,752 - BT1275 0.26 -2.3
1,585,768 - BT1275 0.27 -1.2
1,585,768 - BT1275 0.27 -0.9
1,585,768 - BT1275 0.27 +0.3
1,585,773 + BT1275 0.28 -0.2
1,585,773 + BT1275 0.28 +1.7
1,585,774 - BT1275 0.28 -2.0
1,585,787 - BT1275 0.29 +0.3
1,585,828 - BT1275 0.31 -1.2
1,585,877 + BT1275 0.35 -1.2
1,585,881 - BT1275 0.35 -0.7
1,586,070 + BT1275 0.48 -0.7
1,586,208 + BT1275 0.58 -2.2
1,586,257 - BT1275 0.61 -1.7
1,586,352 - BT1275 0.68 +2.3
1,586,357 - BT1275 0.68 -1.4
1,586,399 - BT1275 0.71 +0.3
1,586,520 + BT1275 0.80 +0.3
1,586,521 - BT1275 0.80 -1.0
1,586,764 - -1.2
1,586,767 - -1.2
1,586,790 + -0.5
1,586,791 - -0.7
1,586,791 - +0.3
1,586,915 - BT1276 0.25 +0.9
1,586,950 + BT1276 0.34 -0.4
1,586,951 - BT1276 0.34 +0.3
1,586,951 - BT1276 0.34 +0.3
1,586,951 - BT1276 0.34 -1.2
1,586,983 + BT1276 0.42 -1.7
1,586,983 + BT1276 0.42 -2.0
1,587,006 + BT1276 0.48 +0.0
1,587,006 + BT1276 0.48 +0.3
1,587,196 + +1.3
1,587,200 + +0.3
1,587,238 + -0.2
1,587,238 + -0.7
1,587,239 - +0.5
1,587,248 - -3.4
1,587,277 + -1.2
1,587,289 - +1.3
1,587,292 + -1.2
1,587,292 + +0.3
1,587,293 - -1.2
1,587,296 - -2.2
1,587,334 + -0.7
1,587,484 - BT1277 0.20 -1.2
1,587,504 - BT1277 0.21 -2.2
1,587,551 + BT1277 0.25 -1.8
1,587,572 + BT1277 0.26 -1.0
1,587,625 + BT1277 0.30 -2.2
1,587,628 - BT1277 0.30 -2.0
1,587,655 + BT1277 0.32 +0.3
1,587,717 + BT1277 0.37 -0.7
1,587,718 - BT1277 0.37 -1.1
1,587,740 - BT1277 0.39 -1.8

Or see this region's nucleotide sequence