Experiment: Amylopectin from Potato; autoclaved 2.5 mg/ml (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tRNA-Gly and BT0954 are separated by 741 nucleotides BT0954 and BT0955 are separated by 6 nucleotides
BT_t18: tRNA-Gly - tRNA-Gly (RefSeq), at 1,163,463 to 1,163,535
tRNA-Gly
BT0954: BT0954 - conserved hypothetical protein (NCBI ptt file), at 1,164,277 to 1,165,689
BT0954
BT0955: BT0955 - conserved hypothetical protein, putative membrane protein (NCBI ptt file), at 1,165,696 to 1,166,685
BT0955
Position (kb)
1164
1165
1166 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 1163.286 kb on + strand at 1163.286 kb on + strand at 1163.287 kb on - strand at 1163.315 kb on + strand at 1163.322 kb on + strand at 1163.334 kb on - strand at 1163.334 kb on - strand at 1163.443 kb on - strand at 1163.507 kb on + strand, within tRNA-Gly at 1163.539 kb on - strand at 1163.545 kb on - strand at 1163.640 kb on - strand at 1163.645 kb on - strand at 1163.646 kb on + strand at 1163.843 kb on + strand at 1163.864 kb on - strand at 1163.908 kb on + strand at 1163.921 kb on + strand at 1163.943 kb on - strand at 1164.068 kb on + strand at 1164.070 kb on + strand at 1164.071 kb on - strand at 1164.103 kb on - strand at 1164.144 kb on - strand at 1164.172 kb on + strand at 1164.264 kb on + strand at 1164.270 kb on - strand at 1164.543 kb on + strand, within BT0954 at 1164.566 kb on + strand, within BT0954 at 1164.593 kb on + strand, within BT0954 at 1164.618 kb on - strand, within BT0954 at 1164.809 kb on - strand, within BT0954 at 1165.063 kb on - strand, within BT0954 at 1165.114 kb on - strand, within BT0954 at 1165.278 kb on + strand, within BT0954 at 1165.429 kb on - strand, within BT0954 at 1165.429 kb on - strand, within BT0954 at 1165.604 kb on - strand at 1165.607 kb on - strand at 1165.613 kb on - strand at 1165.738 kb on - strand at 1165.779 kb on - strand at 1165.908 kb on + strand, within BT0955 at 1166.001 kb on - strand, within BT0955 at 1166.046 kb on - strand, within BT0955 at 1166.070 kb on + strand, within BT0955 at 1166.089 kb on + strand, within BT0955 at 1166.103 kb on + strand, within BT0955 at 1166.111 kb on - strand, within BT0955 at 1166.158 kb on - strand, within BT0955 at 1166.158 kb on - strand, within BT0955 at 1166.194 kb on + strand, within BT0955 at 1166.257 kb on - strand, within BT0955 at 1166.343 kb on - strand, within BT0955 at 1166.591 kb on - strand at 1166.598 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Amylopectin from Potato; autoclaved 2.5 mg/ml (C) remove 1,163,286 + -2.2 1,163,286 + +0.4 1,163,287 - -1.8 1,163,315 + -1.0 1,163,322 + +0.0 1,163,334 - -0.6 1,163,334 - +0.4 1,163,443 - -1.2 1,163,507 + tRNA-Gly BT_t18 0.60 -0.6 1,163,539 - -1.6 1,163,545 - +0.4 1,163,640 - -1.6 1,163,645 - -0.6 1,163,646 + -0.6 1,163,843 + +0.4 1,163,864 - +0.4 1,163,908 + -2.5 1,163,921 + -2.0 1,163,943 - -2.6 1,164,068 + +0.4 1,164,070 + +0.4 1,164,071 - +0.4 1,164,103 - -2.0 1,164,144 - -1.6 1,164,172 + +0.9 1,164,264 + +1.9 1,164,270 - +0.4 1,164,543 + BT0954 0.19 -1.2 1,164,566 + BT0954 0.20 -0.6 1,164,593 + BT0954 0.22 +0.4 1,164,618 - BT0954 0.24 -2.5 1,164,809 - BT0954 0.38 -5.9 1,165,063 - BT0954 0.56 -2.1 1,165,114 - BT0954 0.59 -0.6 1,165,278 + BT0954 0.71 +1.4 1,165,429 - BT0954 0.82 +0.4 1,165,429 - BT0954 0.82 -1.2 1,165,604 - -2.0 1,165,607 - -1.2 1,165,613 - +1.9 1,165,738 - +0.4 1,165,779 - -0.2 1,165,908 + BT0955 0.21 -0.6 1,166,001 - BT0955 0.31 -1.5 1,166,046 - BT0955 0.35 -1.6 1,166,070 + BT0955 0.38 -0.2 1,166,089 + BT0955 0.40 -0.1 1,166,103 + BT0955 0.41 -1.2 1,166,111 - BT0955 0.42 +0.4 1,166,158 - BT0955 0.47 -3.0 1,166,158 - BT0955 0.47 -1.6 1,166,194 + BT0955 0.50 +0.9 1,166,257 - BT0955 0.57 -0.6 1,166,343 - BT0955 0.65 -1.6 1,166,591 - -1.0 1,166,598 - +0.4
Or see this region's nucleotide sequence