Experiment: Amylopectin from Potato; autoclaved 2.5 mg/ml (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0202 and BT0203 are separated by 3 nucleotides BT0203 and BT0204 are separated by 90 nucleotides BT0204 and BT0205 are separated by 114 nucleotides
BT0202: BT0202 - histidinol-phosphate aminotransferase (EC 2.6.1.9) <small>(from data)</small>, at 217,238 to 218,278
BT0202
BT0203: BT0203 - histidine biosynthesis bifunctional protein hisB (NCBI ptt file), at 218,282 to 219,406
BT0203
BT0204: BT0204 - conserved hypothetical protein (NCBI ptt file), at 219,497 to 220,006
BT0204
BT0205: BT0205 - glutamine-dependent NAD+ synthetase (NCBI ptt file), at 220,121 to 222,046
BT0205
Position (kb) 218
219
220 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 217.282 kb on - strand at 217.311 kb on - strand at 217.313 kb on - strand at 217.361 kb on - strand, within BT0202 at 217.447 kb on + strand, within BT0202 at 217.448 kb on - strand, within BT0202 at 217.524 kb on + strand, within BT0202 at 217.524 kb on + strand, within BT0202 at 217.524 kb on + strand, within BT0202 at 217.525 kb on - strand, within BT0202 at 217.545 kb on - strand, within BT0202 at 217.554 kb on - strand, within BT0202 at 217.658 kb on - strand, within BT0202 at 217.731 kb on + strand, within BT0202 at 217.773 kb on - strand, within BT0202 at 217.775 kb on - strand, within BT0202 at 217.941 kb on - strand, within BT0202 at 217.941 kb on - strand, within BT0202 at 217.941 kb on - strand, within BT0202 at 217.992 kb on - strand, within BT0202 at 217.995 kb on - strand, within BT0202 at 218.084 kb on + strand, within BT0202 at 218.085 kb on - strand, within BT0202 at 218.277 kb on - strand at 218.281 kb on + strand at 218.281 kb on + strand at 218.282 kb on - strand at 218.309 kb on - strand at 218.346 kb on - strand at 218.627 kb on - strand, within BT0203 at 218.627 kb on - strand, within BT0203 at 218.870 kb on - strand, within BT0203 at 218.873 kb on + strand, within BT0203 at 218.874 kb on - strand, within BT0203 at 218.874 kb on - strand, within BT0203 at 219.058 kb on - strand, within BT0203 at 219.116 kb on - strand, within BT0203 at 219.172 kb on - strand, within BT0203 at 219.310 kb on - strand at 219.365 kb on - strand at 219.419 kb on + strand at 219.420 kb on - strand at 219.557 kb on - strand, within BT0204 at 219.580 kb on - strand, within BT0204 at 219.592 kb on - strand, within BT0204 at 219.641 kb on + strand, within BT0204 at 219.642 kb on - strand, within BT0204 at 219.712 kb on + strand, within BT0204 at 219.846 kb on + strand, within BT0204 at 219.853 kb on + strand, within BT0204 at 219.854 kb on - strand, within BT0204 at 219.857 kb on + strand, within BT0204 at 219.863 kb on - strand, within BT0204 at 219.863 kb on - strand, within BT0204 at 219.935 kb on - strand, within BT0204 at 219.946 kb on + strand, within BT0204 at 219.947 kb on - strand, within BT0204 at 219.997 kb on - strand at 220.020 kb on - strand at 220.038 kb on - strand at 220.080 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Amylopectin from Potato; autoclaved 2.5 mg/ml (C) remove 217,282 - -2.7 217,311 - -2.2 217,313 - -0.7 217,361 - BT0202 0.12 -0.7 217,447 + BT0202 0.20 -2.2 217,448 - BT0202 0.20 -0.7 217,524 + BT0202 0.27 -0.7 217,524 + BT0202 0.27 +0.3 217,524 + BT0202 0.27 -3.4 217,525 - BT0202 0.28 -2.0 217,545 - BT0202 0.29 -1.2 217,554 - BT0202 0.30 -4.7 217,658 - BT0202 0.40 -3.2 217,731 + BT0202 0.47 -2.0 217,773 - BT0202 0.51 -2.2 217,775 - BT0202 0.52 -3.0 217,941 - BT0202 0.68 -0.7 217,941 - BT0202 0.68 -3.0 217,941 - BT0202 0.68 -3.1 217,992 - BT0202 0.72 -2.0 217,995 - BT0202 0.73 +0.3 218,084 + BT0202 0.81 -3.1 218,085 - BT0202 0.81 -3.0 218,277 - -2.7 218,281 + -1.0 218,281 + -0.7 218,282 - -2.0 218,309 - -4.3 218,346 - -6.4 218,627 - BT0203 0.31 -3.8 218,627 - BT0203 0.31 +0.3 218,870 - BT0203 0.52 -2.0 218,873 + BT0203 0.53 +0.3 218,874 - BT0203 0.53 -1.2 218,874 - BT0203 0.53 -1.2 219,058 - BT0203 0.69 -1.2 219,116 - BT0203 0.74 -0.7 219,172 - BT0203 0.79 -2.0 219,310 - -3.0 219,365 - -1.7 219,419 + -2.7 219,420 - -1.7 219,557 - BT0204 0.12 -0.4 219,580 - BT0204 0.16 -0.7 219,592 - BT0204 0.19 -1.7 219,641 + BT0204 0.28 +0.3 219,642 - BT0204 0.28 -1.7 219,712 + BT0204 0.42 -1.2 219,846 + BT0204 0.68 -1.4 219,853 + BT0204 0.70 -1.4 219,854 - BT0204 0.70 +0.3 219,857 + BT0204 0.71 -0.1 219,863 - BT0204 0.72 -0.7 219,863 - BT0204 0.72 -0.2 219,935 - BT0204 0.86 +0.3 219,946 + BT0204 0.88 -2.1 219,947 - BT0204 0.88 -1.2 219,997 - -0.7 220,020 - -1.2 220,038 - -0.7 220,080 - -0.7
Or see this region's nucleotide sequence