Experiment: Amylopectin from Potato; autoclaved 2.5 mg/ml (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2648 and BT2649 overlap by 4 nucleotides BT2649 and BT2650 are separated by 97 nucleotides BT2650 and BT2651 are separated by 57 nucleotides BT2651 and BT2652 are separated by 347 nucleotides BT2652 and BT2653 are separated by 10 nucleotides
BT2648: BT2648 - hypothetical protein (NCBI ptt file), at 3,301,054 to 3,301,755
BT2648
BT2649: BT2649 - ThiF family protein, ubiquitin-activating enzyme (NCBI ptt file), at 3,301,752 to 3,302,549
BT2649
BT2650: BT2650 - conserved hypothetical protein (NCBI ptt file), at 3,302,647 to 3,303,348
BT2650
BT2651: BT2651 - conserved hypothetical protein (NCBI ptt file), at 3,303,406 to 3,303,699
BT2651
BT2652: BT2652 - conserved hypothetical protein (NCBI ptt file), at 3,304,047 to 3,304,334
BT2652
BT2653: BT2653 - hypothetical protein (NCBI ptt file), at 3,304,345 to 3,304,638
BT2653
Position (kb)
3302
3303
3304 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3301.715 kb on + strand at 3301.745 kb on + strand at 3301.767 kb on + strand at 3301.780 kb on - strand at 3301.795 kb on - strand at 3301.903 kb on + strand, within BT2649 at 3301.904 kb on - strand, within BT2649 at 3301.904 kb on - strand, within BT2649 at 3301.948 kb on + strand, within BT2649 at 3301.948 kb on + strand, within BT2649 at 3301.949 kb on - strand, within BT2649 at 3302.066 kb on - strand, within BT2649 at 3302.260 kb on + strand, within BT2649 at 3302.260 kb on + strand, within BT2649 at 3302.261 kb on - strand, within BT2649 at 3302.261 kb on - strand, within BT2649 at 3302.282 kb on - strand, within BT2649 at 3302.350 kb on - strand, within BT2649 at 3302.422 kb on - strand, within BT2649 at 3302.434 kb on + strand, within BT2649 at 3302.461 kb on + strand, within BT2649 at 3302.462 kb on - strand, within BT2649 at 3302.505 kb on + strand at 3302.505 kb on + strand at 3302.505 kb on + strand at 3302.505 kb on + strand at 3302.505 kb on + strand at 3302.506 kb on - strand at 3302.506 kb on - strand at 3302.507 kb on + strand at 3302.508 kb on - strand at 3302.545 kb on + strand at 3302.548 kb on - strand at 3302.628 kb on + strand at 3302.629 kb on - strand at 3302.721 kb on - strand, within BT2650 at 3302.748 kb on - strand, within BT2650 at 3302.889 kb on + strand, within BT2650 at 3302.890 kb on - strand, within BT2650 at 3302.940 kb on + strand, within BT2650 at 3302.941 kb on - strand, within BT2650 at 3302.950 kb on + strand, within BT2650 at 3302.950 kb on + strand, within BT2650 at 3302.951 kb on - strand, within BT2650 at 3303.012 kb on + strand, within BT2650 at 3303.012 kb on + strand, within BT2650 at 3303.012 kb on + strand, within BT2650 at 3303.030 kb on + strand, within BT2650 at 3303.033 kb on - strand, within BT2650 at 3303.123 kb on + strand, within BT2650 at 3303.224 kb on + strand, within BT2650 at 3303.225 kb on - strand, within BT2650 at 3303.226 kb on + strand, within BT2650 at 3303.227 kb on - strand, within BT2650 at 3303.227 kb on - strand, within BT2650 at 3303.229 kb on - strand, within BT2650 at 3303.229 kb on - strand, within BT2650 at 3303.229 kb on - strand, within BT2650 at 3303.391 kb on + strand at 3303.394 kb on - strand at 3303.488 kb on + strand, within BT2651 at 3303.654 kb on + strand, within BT2651 at 3303.654 kb on + strand, within BT2651 at 3303.684 kb on + strand at 3303.684 kb on + strand at 3303.685 kb on - strand at 3303.688 kb on + strand at 3303.688 kb on + strand at 3303.688 kb on + strand at 3303.688 kb on + strand at 3303.688 kb on + strand at 3303.689 kb on - strand at 3303.693 kb on - strand at 3303.693 kb on - strand at 3303.738 kb on + strand at 3303.756 kb on + strand at 3303.764 kb on + strand at 3303.766 kb on + strand at 3303.766 kb on + strand at 3303.769 kb on + strand at 3303.769 kb on + strand at 3303.769 kb on + strand at 3303.848 kb on + strand at 3303.899 kb on + strand at 3304.015 kb on + strand at 3304.138 kb on + strand, within BT2652 at 3304.190 kb on + strand, within BT2652 at 3304.199 kb on + strand, within BT2652 at 3304.299 kb on + strand, within BT2652
Per-strain Table
Position Strand Gene LocusTag Fraction Amylopectin from Potato; autoclaved 2.5 mg/ml (C) remove 3,301,715 + -1.3 3,301,745 + +0.3 3,301,767 + -1.8 3,301,780 - -1.0 3,301,795 - -2.7 3,301,903 + BT2649 0.19 -1.9 3,301,904 - BT2649 0.19 -0.1 3,301,904 - BT2649 0.19 +1.3 3,301,948 + BT2649 0.25 -2.3 3,301,948 + BT2649 0.25 -1.6 3,301,949 - BT2649 0.25 +0.9 3,302,066 - BT2649 0.39 -1.3 3,302,260 + BT2649 0.64 -2.9 3,302,260 + BT2649 0.64 -0.9 3,302,261 - BT2649 0.64 -2.7 3,302,261 - BT2649 0.64 +0.6 3,302,282 - BT2649 0.66 -1.6 3,302,350 - BT2649 0.75 +0.3 3,302,422 - BT2649 0.84 -1.3 3,302,434 + BT2649 0.85 -1.3 3,302,461 + BT2649 0.89 -1.7 3,302,462 - BT2649 0.89 -1.8 3,302,505 + -3.4 3,302,505 + -1.7 3,302,505 + -1.7 3,302,505 + +2.3 3,302,505 + -1.3 3,302,506 - -0.7 3,302,506 - +0.3 3,302,507 + -0.7 3,302,508 - -1.3 3,302,545 + -1.3 3,302,548 - -2.3 3,302,628 + -1.3 3,302,629 - -1.5 3,302,721 - BT2650 0.11 -1.7 3,302,748 - BT2650 0.14 -0.5 3,302,889 + BT2650 0.34 +0.3 3,302,890 - BT2650 0.35 +0.3 3,302,940 + BT2650 0.42 -1.3 3,302,941 - BT2650 0.42 +0.3 3,302,950 + BT2650 0.43 -1.5 3,302,950 + BT2650 0.43 +0.3 3,302,951 - BT2650 0.43 -1.3 3,303,012 + BT2650 0.52 -0.1 3,303,012 + BT2650 0.52 -1.8 3,303,012 + BT2650 0.52 +1.3 3,303,030 + BT2650 0.55 -0.1 3,303,033 - BT2650 0.55 +0.3 3,303,123 + BT2650 0.68 -0.7 3,303,224 + BT2650 0.82 -1.7 3,303,225 - BT2650 0.82 -0.7 3,303,226 + BT2650 0.82 -1.5 3,303,227 - BT2650 0.83 -1.7 3,303,227 - BT2650 0.83 +0.3 3,303,229 - BT2650 0.83 +0.3 3,303,229 - BT2650 0.83 -0.3 3,303,229 - BT2650 0.83 +1.3 3,303,391 + -1.7 3,303,394 - -2.5 3,303,488 + BT2651 0.28 -1.2 3,303,654 + BT2651 0.84 -2.3 3,303,654 + BT2651 0.84 -1.6 3,303,684 + -2.5 3,303,684 + -2.6 3,303,685 - +1.3 3,303,688 + +1.9 3,303,688 + -1.7 3,303,688 + +0.3 3,303,688 + -1.7 3,303,688 + -0.3 3,303,689 - +0.3 3,303,693 - -2.0 3,303,693 - +1.3 3,303,738 + -2.1 3,303,756 + -1.3 3,303,764 + -1.8 3,303,766 + -0.1 3,303,766 + -2.9 3,303,769 + -0.8 3,303,769 + +0.3 3,303,769 + -1.7 3,303,848 + -2.1 3,303,899 + +0.3 3,304,015 + -0.5 3,304,138 + BT2652 0.32 -1.7 3,304,190 + BT2652 0.50 -1.1 3,304,199 + BT2652 0.53 -2.1 3,304,299 + BT2652 0.88 -2.3
Or see this region's nucleotide sequence