Experiment: Amylopectin from Potato; autoclaved 2.5 mg/ml (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1613 and BT1614 are separated by 47 nucleotides BT1614 and BT1615 are separated by 42 nucleotides BT1615 and BT1616 are separated by 55 nucleotides
BT1613: BT1613 - conserved hypothetical protein (NCBI ptt file), at 1,983,876 to 1,984,457
BT1613
BT1614: BT1614 - hypothetical protein (NCBI ptt file), at 1,984,505 to 1,984,738
BT1614
BT1615: BT1615 - aminoacyl-histidine dipeptidase (NCBI ptt file), at 1,984,781 to 1,986,331
BT1615
BT1616: BT1616 - conserved hypothetical protein (NCBI ptt file), at 1,986,387 to 1,987,508
BT1616
Position (kb)
1984
1985
1986
1987 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1983.825 kb on + strand at 1983.825 kb on + strand at 1983.877 kb on + strand at 1983.896 kb on - strand at 1983.896 kb on - strand at 1983.915 kb on + strand at 1983.935 kb on + strand, within BT1613 at 1983.935 kb on + strand, within BT1613 at 1983.936 kb on - strand, within BT1613 at 1983.937 kb on + strand, within BT1613 at 1983.937 kb on + strand, within BT1613 at 1983.938 kb on - strand, within BT1613 at 1983.938 kb on - strand, within BT1613 at 1983.945 kb on - strand, within BT1613 at 1983.992 kb on + strand, within BT1613 at 1983.992 kb on + strand, within BT1613 at 1984.008 kb on - strand, within BT1613 at 1984.010 kb on + strand, within BT1613 at 1984.058 kb on + strand, within BT1613 at 1984.059 kb on - strand, within BT1613 at 1984.064 kb on + strand, within BT1613 at 1984.064 kb on + strand, within BT1613 at 1984.065 kb on - strand, within BT1613 at 1984.094 kb on + strand, within BT1613 at 1984.141 kb on + strand, within BT1613 at 1984.141 kb on + strand, within BT1613 at 1984.142 kb on - strand, within BT1613 at 1984.303 kb on + strand, within BT1613 at 1984.304 kb on - strand, within BT1613 at 1984.304 kb on - strand, within BT1613 at 1984.320 kb on - strand, within BT1613 at 1984.320 kb on - strand, within BT1613 at 1984.323 kb on + strand, within BT1613 at 1984.333 kb on + strand, within BT1613 at 1984.334 kb on - strand, within BT1613 at 1984.334 kb on - strand, within BT1613 at 1984.380 kb on - strand, within BT1613 at 1984.395 kb on - strand, within BT1613 at 1984.447 kb on - strand at 1984.463 kb on - strand at 1984.468 kb on - strand at 1984.513 kb on + strand at 1984.513 kb on + strand at 1984.686 kb on - strand, within BT1614 at 1984.686 kb on - strand, within BT1614 at 1984.718 kb on + strand at 1984.846 kb on + strand at 1984.847 kb on - strand at 1984.847 kb on - strand at 1984.975 kb on - strand, within BT1615 at 1985.013 kb on + strand, within BT1615 at 1985.013 kb on + strand, within BT1615 at 1985.083 kb on - strand, within BT1615 at 1985.128 kb on - strand, within BT1615 at 1985.227 kb on - strand, within BT1615 at 1985.484 kb on + strand, within BT1615 at 1985.525 kb on - strand, within BT1615 at 1985.556 kb on + strand, within BT1615 at 1985.655 kb on - strand, within BT1615 at 1985.655 kb on - strand, within BT1615 at 1985.659 kb on + strand, within BT1615 at 1985.710 kb on + strand, within BT1615 at 1985.727 kb on + strand, within BT1615 at 1985.813 kb on - strand, within BT1615 at 1985.815 kb on + strand, within BT1615 at 1985.816 kb on - strand, within BT1615 at 1985.816 kb on - strand, within BT1615 at 1985.840 kb on + strand, within BT1615 at 1985.841 kb on - strand, within BT1615 at 1985.859 kb on + strand, within BT1615 at 1985.859 kb on + strand, within BT1615 at 1985.859 kb on + strand, within BT1615 at 1985.859 kb on + strand, within BT1615 at 1985.860 kb on - strand, within BT1615 at 1985.860 kb on - strand, within BT1615 at 1985.861 kb on + strand, within BT1615 at 1985.861 kb on + strand, within BT1615 at 1985.862 kb on - strand, within BT1615 at 1985.878 kb on + strand, within BT1615 at 1985.892 kb on + strand, within BT1615 at 1985.971 kb on + strand, within BT1615 at 1986.127 kb on - strand, within BT1615 at 1986.191 kb on + strand at 1986.201 kb on + strand at 1986.223 kb on + strand at 1986.351 kb on - strand at 1986.406 kb on + strand at 1986.407 kb on - strand at 1986.429 kb on + strand at 1986.430 kb on - strand at 1986.430 kb on - strand at 1986.431 kb on + strand at 1986.646 kb on - strand, within BT1616 at 1986.654 kb on + strand, within BT1616 at 1986.675 kb on - strand, within BT1616 at 1986.754 kb on + strand, within BT1616 at 1986.754 kb on + strand, within BT1616 at 1986.758 kb on - strand, within BT1616 at 1986.805 kb on - strand, within BT1616 at 1986.810 kb on - strand, within BT1616 at 1986.810 kb on - strand, within BT1616 at 1986.855 kb on + strand, within BT1616 at 1986.868 kb on + strand, within BT1616 at 1986.878 kb on - strand, within BT1616 at 1987.162 kb on + strand, within BT1616 at 1987.166 kb on - strand, within BT1616 at 1987.166 kb on - strand, within BT1616 at 1987.218 kb on + strand, within BT1616 at 1987.265 kb on + strand, within BT1616 at 1987.271 kb on + strand, within BT1616 at 1987.319 kb on - strand, within BT1616
Per-strain Table
Position Strand Gene LocusTag Fraction Amylopectin from Potato; autoclaved 2.5 mg/ml (C) remove 1,983,825 + +0.1 1,983,825 + +0.3 1,983,877 + -0.9 1,983,896 - -1.5 1,983,896 - -0.9 1,983,915 + +1.7 1,983,935 + BT1613 0.10 +2.1 1,983,935 + BT1613 0.10 +0.1 1,983,936 - BT1613 0.10 +0.1 1,983,937 + BT1613 0.10 +0.1 1,983,937 + BT1613 0.10 -0.6 1,983,938 - BT1613 0.11 +0.1 1,983,938 - BT1613 0.11 -0.9 1,983,945 - BT1613 0.12 -0.5 1,983,992 + BT1613 0.20 -1.5 1,983,992 + BT1613 0.20 +0.1 1,984,008 - BT1613 0.23 +2.4 1,984,010 + BT1613 0.23 -1.1 1,984,058 + BT1613 0.31 -0.1 1,984,059 - BT1613 0.31 -1.9 1,984,064 + BT1613 0.32 +0.1 1,984,064 + BT1613 0.32 -1.5 1,984,065 - BT1613 0.32 -0.5 1,984,094 + BT1613 0.37 +0.1 1,984,141 + BT1613 0.46 -0.9 1,984,141 + BT1613 0.46 +0.1 1,984,142 - BT1613 0.46 +0.1 1,984,303 + BT1613 0.73 -1.5 1,984,304 - BT1613 0.74 +0.1 1,984,304 - BT1613 0.74 +1.7 1,984,320 - BT1613 0.76 +1.1 1,984,320 - BT1613 0.76 -0.9 1,984,323 + BT1613 0.77 -1.5 1,984,333 + BT1613 0.79 +0.1 1,984,334 - BT1613 0.79 -1.5 1,984,334 - BT1613 0.79 +1.1 1,984,380 - BT1613 0.87 -0.9 1,984,395 - BT1613 0.89 -2.1 1,984,447 - -0.9 1,984,463 - +1.4 1,984,468 - -1.5 1,984,513 + -2.2 1,984,513 + +0.1 1,984,686 - BT1614 0.77 +0.1 1,984,686 - BT1614 0.77 -1.5 1,984,718 + +0.1 1,984,846 + -0.2 1,984,847 - +0.7 1,984,847 - +0.1 1,984,975 - BT1615 0.13 -0.9 1,985,013 + BT1615 0.15 -1.5 1,985,013 + BT1615 0.15 +0.1 1,985,083 - BT1615 0.19 -0.1 1,985,128 - BT1615 0.22 -1.5 1,985,227 - BT1615 0.29 +0.5 1,985,484 + BT1615 0.45 +0.1 1,985,525 - BT1615 0.48 -1.5 1,985,556 + BT1615 0.50 -1.5 1,985,655 - BT1615 0.56 -1.9 1,985,655 - BT1615 0.56 -1.2 1,985,659 + BT1615 0.57 -1.9 1,985,710 + BT1615 0.60 +1.7 1,985,727 + BT1615 0.61 +1.1 1,985,813 - BT1615 0.67 -1.5 1,985,815 + BT1615 0.67 -0.5 1,985,816 - BT1615 0.67 -1.5 1,985,816 - BT1615 0.67 -1.2 1,985,840 + BT1615 0.68 +2.9 1,985,841 - BT1615 0.68 +0.1 1,985,859 + BT1615 0.70 -1.2 1,985,859 + BT1615 0.70 +0.1 1,985,859 + BT1615 0.70 -1.9 1,985,859 + BT1615 0.70 -0.9 1,985,860 - BT1615 0.70 -2.2 1,985,860 - BT1615 0.70 -2.4 1,985,861 + BT1615 0.70 -0.5 1,985,861 + BT1615 0.70 +0.1 1,985,862 - BT1615 0.70 -1.5 1,985,878 + BT1615 0.71 +2.1 1,985,892 + BT1615 0.72 -0.9 1,985,971 + BT1615 0.77 -1.1 1,986,127 - BT1615 0.87 -1.2 1,986,191 + -0.6 1,986,201 + -0.2 1,986,223 + +0.1 1,986,351 - -0.7 1,986,406 + +0.1 1,986,407 - -2.5 1,986,429 + -0.4 1,986,430 - -0.9 1,986,430 - -2.6 1,986,431 + -0.5 1,986,646 - BT1616 0.23 +0.1 1,986,654 + BT1616 0.24 -0.6 1,986,675 - BT1616 0.26 +0.1 1,986,754 + BT1616 0.33 -0.9 1,986,754 + BT1616 0.33 -0.3 1,986,758 - BT1616 0.33 +0.1 1,986,805 - BT1616 0.37 -0.6 1,986,810 - BT1616 0.38 +0.1 1,986,810 - BT1616 0.38 -0.5 1,986,855 + BT1616 0.42 -1.7 1,986,868 + BT1616 0.43 +0.1 1,986,878 - BT1616 0.44 -0.9 1,987,162 + BT1616 0.69 -1.2 1,987,166 - BT1616 0.69 -0.9 1,987,166 - BT1616 0.69 -0.9 1,987,218 + BT1616 0.74 -0.8 1,987,265 + BT1616 0.78 -1.5 1,987,271 + BT1616 0.79 +0.4 1,987,319 - BT1616 0.83 +0.7
Or see this region's nucleotide sequence