Experiment: Pullulan; autoclaved 2.5 mg/ml (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1616 and BT1617 are separated by 91 nucleotides BT1617 and BT1618 are separated by 78 nucleotides BT1618 and BT1619 are separated by 113 nucleotides
BT1616: BT1616 - conserved hypothetical protein (NCBI ptt file), at 1,986,387 to 1,987,508
BT1616
BT1617: BT1617 - RNA polymerase ECF-type sigma factor (NCBI ptt file), at 1,987,600 to 1,988,163
BT1617
BT1618: BT1618 - putative anti-sigma factor (NCBI ptt file), at 1,988,242 to 1,989,249
BT1618
BT1619: BT1619 - putative outer membrane protein, probably involved in nutrient binding (NCBI ptt file), at 1,989,363 to 1,992,815
BT1619
Position (kb)
1988
1989
1990 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1987.265 kb on + strand, within BT1616 at 1987.271 kb on + strand, within BT1616 at 1987.319 kb on - strand, within BT1616 at 1987.384 kb on + strand, within BT1616 at 1987.427 kb on + strand at 1987.431 kb on + strand at 1987.431 kb on + strand at 1987.500 kb on - strand at 1987.500 kb on - strand at 1987.500 kb on - strand at 1987.501 kb on + strand at 1987.532 kb on - strand at 1987.635 kb on + strand at 1987.681 kb on + strand, within BT1617 at 1987.685 kb on - strand, within BT1617 at 1987.704 kb on + strand, within BT1617 at 1987.794 kb on + strand, within BT1617 at 1987.869 kb on - strand, within BT1617 at 1987.906 kb on + strand, within BT1617 at 1988.019 kb on + strand, within BT1617 at 1988.020 kb on - strand, within BT1617 at 1988.116 kb on - strand at 1988.172 kb on - strand at 1988.211 kb on - strand at 1988.211 kb on - strand at 1988.215 kb on - strand at 1988.473 kb on + strand, within BT1618 at 1988.474 kb on - strand, within BT1618 at 1988.475 kb on + strand, within BT1618 at 1988.476 kb on - strand, within BT1618 at 1988.476 kb on - strand, within BT1618 at 1988.507 kb on + strand, within BT1618 at 1988.508 kb on - strand, within BT1618 at 1988.529 kb on + strand, within BT1618 at 1988.530 kb on - strand, within BT1618 at 1988.532 kb on - strand, within BT1618 at 1988.542 kb on + strand, within BT1618 at 1988.543 kb on - strand, within BT1618 at 1988.547 kb on - strand, within BT1618 at 1988.547 kb on - strand, within BT1618 at 1988.548 kb on + strand, within BT1618 at 1988.549 kb on - strand, within BT1618 at 1988.551 kb on - strand, within BT1618 at 1988.552 kb on + strand, within BT1618 at 1988.553 kb on - strand, within BT1618 at 1988.553 kb on - strand, within BT1618 at 1988.591 kb on - strand, within BT1618 at 1988.664 kb on - strand, within BT1618 at 1988.664 kb on - strand, within BT1618 at 1988.705 kb on + strand, within BT1618 at 1988.707 kb on + strand, within BT1618 at 1988.895 kb on + strand, within BT1618 at 1988.896 kb on - strand, within BT1618 at 1988.930 kb on - strand, within BT1618 at 1989.057 kb on - strand, within BT1618 at 1989.058 kb on + strand, within BT1618 at 1989.065 kb on + strand, within BT1618 at 1989.066 kb on - strand, within BT1618 at 1989.066 kb on - strand, within BT1618 at 1989.104 kb on + strand, within BT1618 at 1989.105 kb on - strand, within BT1618 at 1989.105 kb on - strand, within BT1618 at 1989.105 kb on - strand, within BT1618 at 1989.105 kb on - strand, within BT1618 at 1989.105 kb on - strand, within BT1618 at 1989.107 kb on - strand, within BT1618 at 1989.109 kb on - strand, within BT1618 at 1989.185 kb on - strand at 1989.185 kb on - strand at 1989.185 kb on - strand at 1989.186 kb on + strand at 1989.187 kb on - strand at 1989.187 kb on - strand at 1989.326 kb on - strand at 1989.342 kb on + strand at 1989.389 kb on - strand at 1989.436 kb on + strand at 1989.452 kb on - strand at 1989.456 kb on + strand at 1989.543 kb on - strand at 1989.677 kb on + strand at 1989.678 kb on - strand at 1989.681 kb on - strand at 1989.726 kb on - strand, within BT1619 at 1989.749 kb on - strand, within BT1619 at 1989.766 kb on + strand, within BT1619 at 1989.790 kb on + strand, within BT1619 at 1989.953 kb on - strand, within BT1619 at 1989.969 kb on - strand, within BT1619 at 1989.978 kb on - strand, within BT1619 at 1990.040 kb on - strand, within BT1619 at 1990.049 kb on - strand, within BT1619 at 1990.167 kb on + strand, within BT1619
Per-strain Table
Position Strand Gene LocusTag Fraction Pullulan; autoclaved 2.5 mg/ml (C) remove 1,987,265 + BT1616 0.78 -2.2 1,987,271 + BT1616 0.79 -0.1 1,987,319 - BT1616 0.83 +0.5 1,987,384 + BT1616 0.89 -0.6 1,987,427 + -2.5 1,987,431 + -1.4 1,987,431 + -1.2 1,987,500 - -1.6 1,987,500 - -1.1 1,987,500 - -2.1 1,987,501 + -2.1 1,987,532 - -0.1 1,987,635 + -0.5 1,987,681 + BT1617 0.14 -1.3 1,987,685 - BT1617 0.15 -1.6 1,987,704 + BT1617 0.18 -1.6 1,987,794 + BT1617 0.34 -0.9 1,987,869 - BT1617 0.48 -1.9 1,987,906 + BT1617 0.54 -1.5 1,988,019 + BT1617 0.74 -0.1 1,988,020 - BT1617 0.74 -1.6 1,988,116 - -1.7 1,988,172 - -1.0 1,988,211 - -2.1 1,988,211 - -1.9 1,988,215 - +1.3 1,988,473 + BT1618 0.23 -2.1 1,988,474 - BT1618 0.23 -1.9 1,988,475 + BT1618 0.23 -0.1 1,988,476 - BT1618 0.23 -2.4 1,988,476 - BT1618 0.23 -0.1 1,988,507 + BT1618 0.26 +0.9 1,988,508 - BT1618 0.26 -1.6 1,988,529 + BT1618 0.28 -2.1 1,988,530 - BT1618 0.29 -1.1 1,988,532 - BT1618 0.29 -0.9 1,988,542 + BT1618 0.30 -0.5 1,988,543 - BT1618 0.30 -1.9 1,988,547 - BT1618 0.30 +1.9 1,988,547 - BT1618 0.30 -1.9 1,988,548 + BT1618 0.30 -0.1 1,988,549 - BT1618 0.30 +0.9 1,988,551 - BT1618 0.31 -2.1 1,988,552 + BT1618 0.31 -2.5 1,988,553 - BT1618 0.31 -1.4 1,988,553 - BT1618 0.31 -1.4 1,988,591 - BT1618 0.35 -2.1 1,988,664 - BT1618 0.42 -1.9 1,988,664 - BT1618 0.42 -1.1 1,988,705 + BT1618 0.46 -0.1 1,988,707 + BT1618 0.46 -0.1 1,988,895 + BT1618 0.65 +0.9 1,988,896 - BT1618 0.65 -1.1 1,988,930 - BT1618 0.68 -0.1 1,989,057 - BT1618 0.81 -2.0 1,989,058 + BT1618 0.81 -1.5 1,989,065 + BT1618 0.82 -2.4 1,989,066 - BT1618 0.82 -1.0 1,989,066 - BT1618 0.82 +1.9 1,989,104 + BT1618 0.86 -1.4 1,989,105 - BT1618 0.86 -1.6 1,989,105 - BT1618 0.86 -0.6 1,989,105 - BT1618 0.86 -2.1 1,989,105 - BT1618 0.86 -1.5 1,989,105 - BT1618 0.86 -0.6 1,989,107 - BT1618 0.86 -3.0 1,989,109 - BT1618 0.86 -1.1 1,989,185 - -0.4 1,989,185 - -2.1 1,989,185 - -1.6 1,989,186 + -1.1 1,989,187 - -0.1 1,989,187 - -1.1 1,989,326 - -1.3 1,989,342 + -1.6 1,989,389 - -0.1 1,989,436 + -0.6 1,989,452 - -1.3 1,989,456 + -1.5 1,989,543 - +0.5 1,989,677 + -1.9 1,989,678 - -2.6 1,989,681 - -1.7 1,989,726 - BT1619 0.11 -0.6 1,989,749 - BT1619 0.11 -0.6 1,989,766 + BT1619 0.12 -0.1 1,989,790 + BT1619 0.12 -0.8 1,989,953 - BT1619 0.17 -2.5 1,989,969 - BT1619 0.18 +0.9 1,989,978 - BT1619 0.18 -1.1 1,990,040 - BT1619 0.20 -1.6 1,990,049 - BT1619 0.20 -2.9 1,990,167 + BT1619 0.23 -1.4
Or see this region's nucleotide sequence