Experiment: Pullulan; autoclaved 2.5 mg/ml (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1404 and BT1405 are separated by 156 nucleotides BT1405 and BT1406 are separated by 7 nucleotides BT1406 and BT1407 overlap by 14 nucleotides
BT1404: BT1404 - putative transcriptional regulator (NCBI ptt file), at 1,732,613 to 1,733,503
BT1404
BT1405: BT1405 - conserved hypothetical protein (NCBI ptt file), at 1,733,660 to 1,734,331
BT1405
BT1406: BT1406 - transcriptional regulator, TetR family (NCBI ptt file), at 1,734,339 to 1,734,962
BT1406
BT1407: BT1407 - activator of (R)-2-hydroxyglutaryl-CoA dehydratase (NCBI ptt file), at 1,734,949 to 1,739,172
BT1407
Position (kb)
1734
1735 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1733.348 kb on + strand, within BT1404 at 1733.356 kb on - strand, within BT1404 at 1733.392 kb on - strand, within BT1404 at 1733.416 kb on + strand at 1733.417 kb on - strand at 1733.440 kb on - strand at 1733.579 kb on + strand at 1733.645 kb on - strand at 1733.647 kb on - strand at 1733.652 kb on - strand at 1733.680 kb on + strand at 1733.687 kb on + strand at 1733.767 kb on + strand, within BT1405 at 1733.767 kb on + strand, within BT1405 at 1733.784 kb on - strand, within BT1405 at 1733.790 kb on + strand, within BT1405 at 1733.899 kb on + strand, within BT1405 at 1733.908 kb on - strand, within BT1405 at 1733.956 kb on + strand, within BT1405 at 1733.957 kb on - strand, within BT1405 at 1733.993 kb on + strand, within BT1405 at 1733.993 kb on + strand, within BT1405 at 1734.006 kb on + strand, within BT1405 at 1734.012 kb on + strand, within BT1405 at 1734.033 kb on + strand, within BT1405 at 1734.034 kb on - strand, within BT1405 at 1734.072 kb on + strand, within BT1405 at 1734.073 kb on - strand, within BT1405 at 1734.121 kb on + strand, within BT1405 at 1734.259 kb on + strand, within BT1405 at 1734.260 kb on - strand, within BT1405 at 1734.329 kb on + strand at 1734.340 kb on + strand at 1734.379 kb on + strand at 1734.380 kb on - strand at 1734.402 kb on - strand, within BT1406 at 1734.479 kb on + strand, within BT1406 at 1734.481 kb on + strand, within BT1406 at 1734.488 kb on + strand, within BT1406 at 1734.490 kb on + strand, within BT1406 at 1734.495 kb on + strand, within BT1406 at 1734.496 kb on - strand, within BT1406 at 1734.572 kb on - strand, within BT1406 at 1734.682 kb on + strand, within BT1406 at 1734.682 kb on + strand, within BT1406 at 1734.738 kb on + strand, within BT1406 at 1734.740 kb on + strand, within BT1406 at 1734.768 kb on + strand, within BT1406 at 1734.774 kb on - strand, within BT1406 at 1734.897 kb on - strand, within BT1406 at 1734.900 kb on + strand at 1734.900 kb on + strand at 1734.944 kb on + strand at 1735.005 kb on + strand at 1735.015 kb on + strand at 1735.016 kb on - strand at 1735.039 kb on - strand at 1735.113 kb on + strand at 1735.242 kb on + strand at 1735.243 kb on - strand at 1735.266 kb on + strand at 1735.297 kb on - strand at 1735.297 kb on - strand at 1735.462 kb on + strand, within BT1407 at 1735.554 kb on + strand, within BT1407 at 1735.558 kb on + strand, within BT1407 at 1735.558 kb on + strand, within BT1407 at 1735.567 kb on - strand, within BT1407 at 1735.570 kb on + strand, within BT1407 at 1735.570 kb on + strand, within BT1407 at 1735.662 kb on - strand, within BT1407 at 1735.745 kb on + strand, within BT1407 at 1735.813 kb on - strand, within BT1407 at 1735.859 kb on + strand, within BT1407
Per-strain Table
Position Strand Gene LocusTag Fraction Pullulan; autoclaved 2.5 mg/ml (C) remove 1,733,348 + BT1404 0.82 +0.0 1,733,356 - BT1404 0.83 +1.0 1,733,392 - BT1404 0.87 +0.0 1,733,416 + -1.6 1,733,417 - -1.0 1,733,440 - -0.2 1,733,579 + -1.8 1,733,645 - -1.0 1,733,647 - -2.0 1,733,652 - -2.0 1,733,680 + -1.8 1,733,687 + -2.3 1,733,767 + BT1405 0.16 +2.3 1,733,767 + BT1405 0.16 -1.1 1,733,784 - BT1405 0.18 +0.0 1,733,790 + BT1405 0.19 -0.9 1,733,899 + BT1405 0.36 -1.4 1,733,908 - BT1405 0.37 +0.0 1,733,956 + BT1405 0.44 -0.4 1,733,957 - BT1405 0.44 -0.5 1,733,993 + BT1405 0.50 -1.6 1,733,993 + BT1405 0.50 -1.2 1,734,006 + BT1405 0.51 -1.0 1,734,012 + BT1405 0.52 -1.2 1,734,033 + BT1405 0.56 -0.5 1,734,034 - BT1405 0.56 -0.6 1,734,072 + BT1405 0.61 +0.0 1,734,073 - BT1405 0.61 -0.6 1,734,121 + BT1405 0.69 -2.1 1,734,259 + BT1405 0.89 -1.0 1,734,260 - BT1405 0.89 +0.0 1,734,329 + -1.6 1,734,340 + +0.0 1,734,379 + -1.0 1,734,380 - +0.4 1,734,402 - BT1406 0.10 -1.6 1,734,479 + BT1406 0.22 -0.4 1,734,481 + BT1406 0.23 -1.2 1,734,488 + BT1406 0.24 -1.6 1,734,490 + BT1406 0.24 +0.0 1,734,495 + BT1406 0.25 -1.0 1,734,496 - BT1406 0.25 +0.0 1,734,572 - BT1406 0.37 -1.2 1,734,682 + BT1406 0.55 -2.0 1,734,682 + BT1406 0.55 -1.7 1,734,738 + BT1406 0.64 -2.8 1,734,740 + BT1406 0.64 +0.0 1,734,768 + BT1406 0.69 -2.1 1,734,774 - BT1406 0.70 +0.0 1,734,897 - BT1406 0.89 -1.5 1,734,900 + -0.4 1,734,900 + -1.6 1,734,944 + -2.0 1,735,005 + -0.6 1,735,015 + +0.0 1,735,016 - -1.6 1,735,039 - -1.5 1,735,113 + -1.0 1,735,242 + +0.0 1,735,243 - -1.3 1,735,266 + -1.8 1,735,297 - +0.0 1,735,297 - -1.0 1,735,462 + BT1407 0.12 -1.0 1,735,554 + BT1407 0.14 -1.0 1,735,558 + BT1407 0.14 +0.0 1,735,558 + BT1407 0.14 -1.6 1,735,567 - BT1407 0.15 -1.0 1,735,570 + BT1407 0.15 -1.0 1,735,570 + BT1407 0.15 -3.2 1,735,662 - BT1407 0.17 -1.0 1,735,745 + BT1407 0.19 +0.0 1,735,813 - BT1407 0.20 +1.0 1,735,859 + BT1407 0.22 +1.2
Or see this region's nucleotide sequence