Experiment: polygalacturonic (ultrapure); autoclaved 2.5 mg/ml (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0134 and BT0135 overlap by 23 nucleotides BT0135 and BT0136 are separated by 152 nucleotides BT0136 and BT0137 are separated by 104 nucleotides
BT0134: BT0134 - conserved hypothetical protein (NCBI ptt file), at 130,680 to 131,063
BT0134
BT0135: BT0135 - conserved hypothetical protein (NCBI ptt file), at 131,041 to 131,823
BT0135
BT0136: BT0136 - hypothetical protein (NCBI ptt file), at 131,976 to 133,586
BT0136
BT0137: BT0137 - conserved hypothetical protein (NCBI ptt file), at 133,691 to 135,616
BT0137
Position (kb) 131
132
133
134 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 130.982 kb on + strand, within BT0134 at 131.044 kb on + strand at 131.045 kb on - strand at 131.077 kb on + strand at 131.083 kb on + strand at 131.124 kb on - strand, within BT0135 at 131.197 kb on + strand, within BT0135 at 131.206 kb on + strand, within BT0135 at 131.242 kb on - strand, within BT0135 at 131.401 kb on - strand, within BT0135 at 131.406 kb on + strand, within BT0135 at 131.419 kb on - strand, within BT0135 at 131.472 kb on + strand, within BT0135 at 131.489 kb on - strand, within BT0135 at 131.569 kb on + strand, within BT0135 at 131.570 kb on - strand, within BT0135 at 131.656 kb on + strand, within BT0135 at 131.726 kb on + strand, within BT0135 at 131.791 kb on - strand at 131.792 kb on + strand at 132.010 kb on + strand at 132.018 kb on - strand at 132.022 kb on + strand at 132.067 kb on + strand at 132.078 kb on - strand at 132.156 kb on + strand, within BT0136 at 132.158 kb on - strand, within BT0136 at 132.161 kb on + strand, within BT0136 at 132.171 kb on - strand, within BT0136 at 132.249 kb on - strand, within BT0136 at 132.278 kb on - strand, within BT0136 at 132.470 kb on + strand, within BT0136 at 132.473 kb on + strand, within BT0136 at 132.519 kb on - strand, within BT0136 at 132.609 kb on + strand, within BT0136 at 132.683 kb on - strand, within BT0136 at 132.684 kb on + strand, within BT0136 at 132.736 kb on - strand, within BT0136 at 132.736 kb on - strand, within BT0136 at 132.747 kb on - strand, within BT0136 at 132.827 kb on + strand, within BT0136 at 132.949 kb on - strand, within BT0136 at 132.962 kb on - strand, within BT0136 at 132.985 kb on - strand, within BT0136 at 133.043 kb on + strand, within BT0136 at 133.083 kb on - strand, within BT0136 at 133.160 kb on + strand, within BT0136 at 133.163 kb on + strand, within BT0136 at 133.200 kb on - strand, within BT0136 at 133.202 kb on + strand, within BT0136 at 133.203 kb on - strand, within BT0136 at 133.250 kb on + strand, within BT0136 at 133.258 kb on - strand, within BT0136 at 133.281 kb on - strand, within BT0136 at 133.409 kb on - strand, within BT0136 at 133.411 kb on - strand, within BT0136 at 133.413 kb on + strand, within BT0136 at 133.416 kb on + strand, within BT0136 at 133.417 kb on - strand, within BT0136 at 133.460 kb on - strand at 133.488 kb on + strand at 133.508 kb on - strand at 133.508 kb on - strand at 133.640 kb on - strand at 133.640 kb on - strand at 133.684 kb on - strand at 133.703 kb on - strand at 133.720 kb on - strand at 133.722 kb on + strand at 133.765 kb on - strand at 133.876 kb on + strand at 133.877 kb on - strand at 133.877 kb on - strand at 133.979 kb on - strand, within BT0137 at 133.979 kb on - strand, within BT0137 at 133.986 kb on + strand, within BT0137 at 134.036 kb on - strand, within BT0137 at 134.036 kb on - strand, within BT0137 at 134.174 kb on + strand, within BT0137 at 134.232 kb on - strand, within BT0137 at 134.232 kb on - strand, within BT0137 at 134.233 kb on + strand, within BT0137 at 134.242 kb on + strand, within BT0137 at 134.250 kb on - strand, within BT0137 at 134.271 kb on - strand, within BT0137 at 134.271 kb on - strand, within BT0137 at 134.274 kb on + strand, within BT0137 at 134.277 kb on - strand, within BT0137 at 134.380 kb on + strand, within BT0137 at 134.418 kb on + strand, within BT0137 at 134.418 kb on + strand, within BT0137 at 134.419 kb on - strand, within BT0137 at 134.421 kb on - strand, within BT0137 at 134.474 kb on - strand, within BT0137 at 134.570 kb on + strand, within BT0137
Per-strain Table
Position Strand Gene LocusTag Fraction polygalacturonic (ultrapure); autoclaved 2.5 mg/ml (C) remove 130,982 + BT0134 0.79 -0.1 131,044 + -1.4 131,045 - +1.3 131,077 + -0.4 131,083 + -0.7 131,124 - BT0135 0.11 -0.6 131,197 + BT0135 0.20 -0.1 131,206 + BT0135 0.21 -0.8 131,242 - BT0135 0.26 -1.5 131,401 - BT0135 0.46 -1.9 131,406 + BT0135 0.47 -0.5 131,419 - BT0135 0.48 -1.6 131,472 + BT0135 0.55 -1.1 131,489 - BT0135 0.57 -1.1 131,569 + BT0135 0.67 -1.2 131,570 - BT0135 0.68 -1.6 131,656 + BT0135 0.79 -0.1 131,726 + BT0135 0.87 -0.8 131,791 - -0.5 131,792 + -1.1 132,010 + -0.1 132,018 - -0.1 132,022 + +0.1 132,067 + -0.9 132,078 - -1.9 132,156 + BT0136 0.11 -0.6 132,158 - BT0136 0.11 -2.4 132,161 + BT0136 0.11 +0.9 132,171 - BT0136 0.12 -1.1 132,249 - BT0136 0.17 +0.9 132,278 - BT0136 0.19 -1.1 132,470 + BT0136 0.31 -1.1 132,473 + BT0136 0.31 +0.9 132,519 - BT0136 0.34 -1.9 132,609 + BT0136 0.39 -1.4 132,683 - BT0136 0.44 -1.9 132,684 + BT0136 0.44 -1.5 132,736 - BT0136 0.47 -1.1 132,736 - BT0136 0.47 -1.4 132,747 - BT0136 0.48 -0.1 132,827 + BT0136 0.53 -1.9 132,949 - BT0136 0.60 -1.6 132,962 - BT0136 0.61 -3.1 132,985 - BT0136 0.63 -2.4 133,043 + BT0136 0.66 -0.1 133,083 - BT0136 0.69 -0.1 133,160 + BT0136 0.73 -0.6 133,163 + BT0136 0.74 -1.1 133,200 - BT0136 0.76 -1.1 133,202 + BT0136 0.76 -1.4 133,203 - BT0136 0.76 -1.2 133,250 + BT0136 0.79 -1.6 133,258 - BT0136 0.80 -2.6 133,281 - BT0136 0.81 -1.8 133,409 - BT0136 0.89 -0.5 133,411 - BT0136 0.89 -0.1 133,413 + BT0136 0.89 -0.1 133,416 + BT0136 0.89 -4.0 133,417 - BT0136 0.89 -1.6 133,460 - -1.1 133,488 + -0.5 133,508 - -0.9 133,508 - -2.9 133,640 - -0.1 133,640 - -1.1 133,684 - +0.4 133,703 - -0.6 133,720 - -1.6 133,722 + -2.6 133,765 - +0.4 133,876 + -0.7 133,877 - -1.6 133,877 - -2.4 133,979 - BT0137 0.15 +0.9 133,979 - BT0137 0.15 +1.9 133,986 + BT0137 0.15 -0.6 134,036 - BT0137 0.18 -0.1 134,036 - BT0137 0.18 -0.1 134,174 + BT0137 0.25 -1.1 134,232 - BT0137 0.28 -0.6 134,232 - BT0137 0.28 -0.6 134,233 + BT0137 0.28 -1.4 134,242 + BT0137 0.29 +0.9 134,250 - BT0137 0.29 -0.1 134,271 - BT0137 0.30 -1.6 134,271 - BT0137 0.30 -0.9 134,274 + BT0137 0.30 -0.2 134,277 - BT0137 0.30 -1.1 134,380 + BT0137 0.36 -0.4 134,418 + BT0137 0.38 -0.1 134,418 + BT0137 0.38 +0.9 134,419 - BT0137 0.38 -0.6 134,421 - BT0137 0.38 +0.4 134,474 - BT0137 0.41 -2.6 134,570 + BT0137 0.46 -1.1
Or see this region's nucleotide sequence