Experiment: KLEB1 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25850 and BWI76_RS25855 overlap by 17 nucleotides BWI76_RS25855 and BWI76_RS25860 are separated by 74 nucleotides BWI76_RS25860 and BWI76_RS25865 overlap by 60 nucleotides BWI76_RS25865 and BWI76_RS25870 are separated by 98 nucleotides BWI76_RS25870 and BWI76_RS25875 are separated by 70 nucleotides
BWI76_RS25850: BWI76_RS25850 - prepilin peptidase, at 5,350,290 to 5,350,766
_RS25850
BWI76_RS25855: BWI76_RS25855 - bacterioferritin, at 5,350,750 to 5,351,226
_RS25855
BWI76_RS25860: BWI76_RS25860 - bacterioferritin-associated ferredoxin, at 5,351,301 to 5,351,495
_RS25860
BWI76_RS25865: BWI76_RS25865 - ferredoxin, at 5,351,436 to 5,351,570
_RS25865
BWI76_RS25870: BWI76_RS25870 - elongation factor Tu, at 5,351,669 to 5,352,853
_RS25870
BWI76_RS25875: BWI76_RS25875 - elongation factor G, at 5,352,924 to 5,355,038
_RS25875
Position (kb) 5351
5352
5353 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 5350.682 kb on + strand, within BWI76_RS25850 at 5350.685 kb on - strand, within BWI76_RS25850 at 5350.888 kb on + strand, within BWI76_RS25855 at 5350.909 kb on + strand, within BWI76_RS25855 at 5351.057 kb on - strand, within BWI76_RS25855 at 5351.075 kb on - strand, within BWI76_RS25855 at 5351.126 kb on + strand, within BWI76_RS25855 at 5351.240 kb on + strand at 5351.283 kb on + strand at 5351.436 kb on - strand at 5351.473 kb on + strand at 5351.479 kb on - strand at 5351.481 kb on - strand at 5351.483 kb on + strand at 5351.491 kb on - strand at 5351.503 kb on - strand, within BWI76_RS25865 at 5351.524 kb on + strand, within BWI76_RS25865 at 5351.589 kb on + strand at 5352.505 kb on - strand, within BWI76_RS25870 at 5352.856 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB1 MOI 0.1 remove 5,350,682 + BWI76_RS25850 0.82 -0.7 5,350,685 - BWI76_RS25850 0.83 -4.1 5,350,888 + BWI76_RS25855 0.29 +1.2 5,350,909 + BWI76_RS25855 0.33 -2.0 5,351,057 - BWI76_RS25855 0.64 -2.5 5,351,075 - BWI76_RS25855 0.68 -0.7 5,351,126 + BWI76_RS25855 0.79 -0.7 5,351,240 + +0.2 5,351,283 + +0.2 5,351,436 - -1.8 5,351,473 + -0.9 5,351,479 - +1.5 5,351,481 - +1.3 5,351,483 + -0.7 5,351,491 - -1.8 5,351,503 - BWI76_RS25865 0.50 -0.4 5,351,524 + BWI76_RS25865 0.65 -0.3 5,351,589 + +0.6 5,352,505 - BWI76_RS25870 0.71 -1.6 5,352,856 + +0.2
Or see this region's nucleotide sequence