Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS25680

Experiment: KLEB1 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS25675 and BWI76_RS25680 are separated by 52 nucleotidesBWI76_RS25680 and BWI76_RS25685 are separated by 21 nucleotides BWI76_RS25675: BWI76_RS25675 - methionyl-tRNA formyltransferase, at 5,330,836 to 5,331,783 _RS25675 BWI76_RS25680: BWI76_RS25680 - 16S rRNA m5C967 methyltransferase, at 5,331,836 to 5,333,125 _RS25680 BWI76_RS25685: BWI76_RS25685 - Trk system potassium transport protein TrkA, at 5,333,147 to 5,334,523 _RS25685 Position (kb) 5331 5332 5333 5334Strain fitness (log2 ratio) -2 -1 0 1at 5331.755 kb on + strandat 5332.107 kb on + strand, within BWI76_RS25680at 5332.349 kb on + strand, within BWI76_RS25680at 5332.352 kb on + strand, within BWI76_RS25680at 5332.481 kb on - strand, within BWI76_RS25680at 5332.546 kb on - strand, within BWI76_RS25680at 5332.651 kb on + strand, within BWI76_RS25680at 5332.882 kb on + strand, within BWI76_RS25680at 5332.911 kb on + strand, within BWI76_RS25680at 5332.911 kb on + strand, within BWI76_RS25680at 5333.008 kb on + strandat 5333.048 kb on + strandat 5333.055 kb on - strandat 5333.098 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB1 MOI 0.1
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5,331,755 + -0.5
5,332,107 + BWI76_RS25680 0.21 -1.8
5,332,349 + BWI76_RS25680 0.40 -0.2
5,332,352 + BWI76_RS25680 0.40 -2.1
5,332,481 - BWI76_RS25680 0.50 -0.4
5,332,546 - BWI76_RS25680 0.55 -1.8
5,332,651 + BWI76_RS25680 0.63 -2.2
5,332,882 + BWI76_RS25680 0.81 -2.2
5,332,911 + BWI76_RS25680 0.83 -0.5
5,332,911 + BWI76_RS25680 0.83 -1.1
5,333,008 + -0.8
5,333,048 + +0.8
5,333,055 - -0.8
5,333,098 + -1.1

Or see this region's nucleotide sequence