Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS25660

Experiment: KLEB1 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS25650 and BWI76_RS25655 are separated by 4 nucleotidesBWI76_RS25655 and BWI76_RS25660 are separated by 27 nucleotidesBWI76_RS25660 and BWI76_RS25665 overlap by 29 nucleotides BWI76_RS25650: BWI76_RS25650 - L-threonylcarbamoyladenylate synthase type 1 TsaC, at 5,327,466 to 5,328,038 _RS25650 BWI76_RS25655: BWI76_RS25655 - DNA topoisomerase type IA zn finger domain-containing protein, at 5,328,043 to 5,328,585 _RS25655 BWI76_RS25660: BWI76_RS25660 - hypothetical protein, at 5,328,613 to 5,329,086 _RS25660 BWI76_RS25665: BWI76_RS25665 - DNA-processing protein DprA, at 5,329,058 to 5,330,182 _RS25665 Position (kb) 5328 5329 5330Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 5328.405 kb on - strand, within BWI76_RS25655at 5328.414 kb on - strand, within BWI76_RS25655at 5328.470 kb on + strandat 5328.492 kb on - strand, within BWI76_RS25655at 5328.520 kb on - strand, within BWI76_RS25655at 5328.763 kb on + strand, within BWI76_RS25660at 5328.866 kb on + strand, within BWI76_RS25660at 5328.951 kb on + strand, within BWI76_RS25660at 5329.015 kb on + strand, within BWI76_RS25660at 5329.023 kb on - strand, within BWI76_RS25660at 5329.055 kb on + strandat 5329.133 kb on - strandat 5329.140 kb on + strandat 5329.153 kb on - strandat 5329.215 kb on + strand, within BWI76_RS25665at 5329.218 kb on - strand, within BWI76_RS25665at 5329.224 kb on - strand, within BWI76_RS25665at 5329.345 kb on - strand, within BWI76_RS25665at 5329.401 kb on - strand, within BWI76_RS25665at 5329.656 kb on + strand, within BWI76_RS25665at 5329.656 kb on + strand, within BWI76_RS25665at 5329.656 kb on + strand, within BWI76_RS25665at 5329.664 kb on - strand, within BWI76_RS25665at 5329.664 kb on - strand, within BWI76_RS25665at 5329.664 kb on - strand, within BWI76_RS25665at 5329.674 kb on - strand, within BWI76_RS25665at 5329.674 kb on - strand, within BWI76_RS25665at 5329.729 kb on - strand, within BWI76_RS25665at 5329.729 kb on - strand, within BWI76_RS25665at 5329.789 kb on - strand, within BWI76_RS25665at 5329.871 kb on - strand, within BWI76_RS25665at 5329.889 kb on + strand, within BWI76_RS25665at 5329.897 kb on - strand, within BWI76_RS25665at 5329.897 kb on - strand, within BWI76_RS25665at 5329.983 kb on + strandat 5330.022 kb on - strand, within BWI76_RS25665

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB1 MOI 0.1
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5,328,405 - BWI76_RS25655 0.67 -1.5
5,328,414 - BWI76_RS25655 0.68 +0.5
5,328,470 + +0.2
5,328,492 - BWI76_RS25655 0.83 +0.1
5,328,520 - BWI76_RS25655 0.88 -1.5
5,328,763 + BWI76_RS25660 0.32 -1.2
5,328,866 + BWI76_RS25660 0.53 -0.5
5,328,951 + BWI76_RS25660 0.71 -1.6
5,329,015 + BWI76_RS25660 0.85 -1.3
5,329,023 - BWI76_RS25660 0.86 -1.8
5,329,055 + -0.1
5,329,133 - -0.5
5,329,140 + -3.2
5,329,153 - -1.3
5,329,215 + BWI76_RS25665 0.14 -0.4
5,329,218 - BWI76_RS25665 0.14 -1.4
5,329,224 - BWI76_RS25665 0.15 -1.7
5,329,345 - BWI76_RS25665 0.26 -0.6
5,329,401 - BWI76_RS25665 0.30 -0.7
5,329,656 + BWI76_RS25665 0.53 +0.0
5,329,656 + BWI76_RS25665 0.53 +0.5
5,329,656 + BWI76_RS25665 0.53 -1.4
5,329,664 - BWI76_RS25665 0.54 +2.4
5,329,664 - BWI76_RS25665 0.54 -1.0
5,329,664 - BWI76_RS25665 0.54 -0.7
5,329,674 - BWI76_RS25665 0.55 +0.2
5,329,674 - BWI76_RS25665 0.55 -1.7
5,329,729 - BWI76_RS25665 0.60 -1.4
5,329,729 - BWI76_RS25665 0.60 +0.7
5,329,789 - BWI76_RS25665 0.65 -0.1
5,329,871 - BWI76_RS25665 0.72 -2.0
5,329,889 + BWI76_RS25665 0.74 -2.0
5,329,897 - BWI76_RS25665 0.75 -2.3
5,329,897 - BWI76_RS25665 0.75 -0.7
5,329,983 + -0.4
5,330,022 - BWI76_RS25665 0.86 -0.3

Or see this region's nucleotide sequence