Experiment: KLEB1 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25420 and BWI76_RS25425 are separated by 412 nucleotides BWI76_RS25425 and BWI76_RS25430 are separated by 354 nucleotides BWI76_RS25430 and BWI76_RS25435 are separated by 103 nucleotides
BWI76_RS25420: BWI76_RS25420 - malate dehydrogenase, at 5,283,233 to 5,284,171
_RS25420
BWI76_RS25425: BWI76_RS25425 - arginine repressor, at 5,284,584 to 5,285,054
_RS25425
BWI76_RS25430: BWI76_RS25430 - membrane protein, at 5,285,409 to 5,285,672
_RS25430
BWI76_RS25435: BWI76_RS25435 - membrane protein, at 5,285,776 to 5,286,042
_RS25435
Position (kb) 5284
5285
5286 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5283.633 kb on + strand, within BWI76_RS25420 at 5283.826 kb on + strand, within BWI76_RS25420 at 5283.844 kb on - strand, within BWI76_RS25420 at 5283.856 kb on - strand, within BWI76_RS25420 at 5284.085 kb on + strand at 5284.092 kb on - strand at 5284.092 kb on - strand at 5284.106 kb on + strand at 5284.278 kb on + strand at 5284.299 kb on - strand at 5284.333 kb on - strand at 5284.335 kb on + strand at 5284.343 kb on - strand at 5284.343 kb on - strand at 5284.380 kb on + strand at 5284.634 kb on + strand, within BWI76_RS25425 at 5284.914 kb on + strand, within BWI76_RS25425 at 5285.025 kb on + strand at 5285.498 kb on + strand, within BWI76_RS25430 at 5285.498 kb on + strand, within BWI76_RS25430 at 5285.506 kb on - strand, within BWI76_RS25430 at 5285.507 kb on - strand, within BWI76_RS25430 at 5285.545 kb on + strand, within BWI76_RS25430 at 5285.620 kb on + strand, within BWI76_RS25430 at 5285.632 kb on + strand, within BWI76_RS25430 at 5285.637 kb on + strand, within BWI76_RS25430 at 5285.717 kb on - strand at 5285.811 kb on + strand, within BWI76_RS25435 at 5285.891 kb on - strand, within BWI76_RS25435 at 5285.908 kb on + strand, within BWI76_RS25435 at 5286.018 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB1 MOI 0.1 remove 5,283,633 + BWI76_RS25420 0.43 -0.4 5,283,826 + BWI76_RS25420 0.63 +0.1 5,283,844 - BWI76_RS25420 0.65 -0.1 5,283,856 - BWI76_RS25420 0.66 -1.1 5,284,085 + -0.6 5,284,092 - +0.2 5,284,092 - -1.1 5,284,106 + -1.3 5,284,278 + -0.5 5,284,299 - -2.0 5,284,333 - -2.3 5,284,335 + -0.6 5,284,343 - -2.0 5,284,343 - +0.2 5,284,380 + +0.1 5,284,634 + BWI76_RS25425 0.11 -2.3 5,284,914 + BWI76_RS25425 0.70 -0.8 5,285,025 + -0.7 5,285,498 + BWI76_RS25430 0.34 -1.6 5,285,498 + BWI76_RS25430 0.34 +0.0 5,285,506 - BWI76_RS25430 0.37 -0.7 5,285,507 - BWI76_RS25430 0.37 -3.0 5,285,545 + BWI76_RS25430 0.52 -0.7 5,285,620 + BWI76_RS25430 0.80 +1.0 5,285,632 + BWI76_RS25430 0.84 -1.2 5,285,637 + BWI76_RS25430 0.86 +0.1 5,285,717 - -1.2 5,285,811 + BWI76_RS25435 0.13 -0.2 5,285,891 - BWI76_RS25435 0.43 +0.1 5,285,908 + BWI76_RS25435 0.49 -1.5 5,286,018 + -0.6
Or see this region's nucleotide sequence