Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS24565

Experiment: KLEB1 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS24555 and BWI76_RS24560 are separated by 242 nucleotidesBWI76_RS24560 and BWI76_RS24565 are separated by 40 nucleotidesBWI76_RS24565 and BWI76_RS24570 are separated by 124 nucleotidesBWI76_RS24570 and BWI76_RS24575 are separated by 86 nucleotidesBWI76_RS24575 and BWI76_RS24580 are separated by 137 nucleotides BWI76_RS24555: BWI76_RS24555 - RNA polymerase sigma factor RpoD, at 5,118,648 to 5,120,492 _RS24555 BWI76_RS24560: BWI76_RS24560 - TetR family transcriptional regulator, at 5,120,735 to 5,121,397 _RS24560 BWI76_RS24565: BWI76_RS24565 - mismatch-specific DNA-glycosylase, at 5,121,438 to 5,121,944 _RS24565 BWI76_RS24570: BWI76_RS24570 - tRNA-Met, at 5,122,069 to 5,122,144 _RS24570 BWI76_RS24575: BWI76_RS24575 - hypothetical protein, at 5,122,231 to 5,122,554 _RS24575 BWI76_RS24580: BWI76_RS24580 - PTS-dependent dihydroxyacetone kinase operon transcriptional regulator DhaR, at 5,122,692 to 5,124,605 _RS24580 Position (kb) 5121 5122Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4at 5120.655 kb on - strandat 5120.655 kb on - strandat 5120.655 kb on - strandat 5120.681 kb on - strandat 5120.692 kb on + strandat 5120.693 kb on + strandat 5120.700 kb on - strandat 5120.701 kb on - strandat 5120.802 kb on - strand, within BWI76_RS24560at 5120.843 kb on - strand, within BWI76_RS24560at 5120.869 kb on - strand, within BWI76_RS24560at 5120.869 kb on - strand, within BWI76_RS24560at 5120.879 kb on - strand, within BWI76_RS24560at 5120.922 kb on - strand, within BWI76_RS24560at 5120.927 kb on - strand, within BWI76_RS24560at 5120.927 kb on - strand, within BWI76_RS24560at 5120.927 kb on - strand, within BWI76_RS24560at 5120.934 kb on - strand, within BWI76_RS24560at 5120.942 kb on - strand, within BWI76_RS24560at 5120.957 kb on - strand, within BWI76_RS24560at 5121.012 kb on + strand, within BWI76_RS24560at 5121.014 kb on - strand, within BWI76_RS24560at 5121.020 kb on - strand, within BWI76_RS24560at 5121.020 kb on - strand, within BWI76_RS24560at 5121.025 kb on - strand, within BWI76_RS24560at 5121.025 kb on - strand, within BWI76_RS24560at 5121.025 kb on - strand, within BWI76_RS24560at 5121.034 kb on - strand, within BWI76_RS24560at 5121.034 kb on - strand, within BWI76_RS24560at 5121.055 kb on - strand, within BWI76_RS24560at 5121.210 kb on - strand, within BWI76_RS24560at 5121.220 kb on + strand, within BWI76_RS24560at 5121.220 kb on + strand, within BWI76_RS24560at 5121.222 kb on + strand, within BWI76_RS24560at 5121.227 kb on + strand, within BWI76_RS24560at 5121.228 kb on - strand, within BWI76_RS24560at 5121.230 kb on - strand, within BWI76_RS24560at 5121.230 kb on - strand, within BWI76_RS24560at 5121.230 kb on - strand, within BWI76_RS24560at 5121.230 kb on - strand, within BWI76_RS24560at 5121.232 kb on + strand, within BWI76_RS24560at 5121.232 kb on + strand, within BWI76_RS24560at 5121.232 kb on + strand, within BWI76_RS24560at 5121.232 kb on + strand, within BWI76_RS24560at 5121.232 kb on + strand, within BWI76_RS24560at 5121.232 kb on + strand, within BWI76_RS24560at 5121.232 kb on + strand, within BWI76_RS24560at 5121.232 kb on + strand, within BWI76_RS24560at 5121.232 kb on + strand, within BWI76_RS24560at 5121.235 kb on - strand, within BWI76_RS24560at 5121.237 kb on + strand, within BWI76_RS24560at 5121.237 kb on + strand, within BWI76_RS24560at 5121.237 kb on + strand, within BWI76_RS24560at 5121.237 kb on + strand, within BWI76_RS24560at 5121.240 kb on - strand, within BWI76_RS24560at 5121.240 kb on - strand, within BWI76_RS24560at 5121.240 kb on - strand, within BWI76_RS24560at 5121.240 kb on - strand, within BWI76_RS24560at 5121.240 kb on - strand, within BWI76_RS24560at 5121.240 kb on - strand, within BWI76_RS24560at 5121.240 kb on - strand, within BWI76_RS24560at 5121.240 kb on - strand, within BWI76_RS24560at 5121.240 kb on - strand, within BWI76_RS24560at 5121.240 kb on - strand, within BWI76_RS24560at 5121.240 kb on - strand, within BWI76_RS24560at 5121.240 kb on - strand, within BWI76_RS24560at 5121.240 kb on - strand, within BWI76_RS24560at 5121.241 kb on - strand, within BWI76_RS24560at 5121.241 kb on - strand, within BWI76_RS24560at 5121.241 kb on - strand, within BWI76_RS24560at 5121.242 kb on + strand, within BWI76_RS24560at 5121.251 kb on - strand, within BWI76_RS24560at 5121.251 kb on - strand, within BWI76_RS24560at 5121.251 kb on - strand, within BWI76_RS24560at 5121.255 kb on - strand, within BWI76_RS24560at 5121.302 kb on + strand, within BWI76_RS24560at 5121.306 kb on - strand, within BWI76_RS24560at 5121.306 kb on - strand, within BWI76_RS24560at 5121.339 kb on + strandat 5121.342 kb on - strandat 5121.344 kb on + strandat 5121.347 kb on - strandat 5121.355 kb on - strandat 5121.399 kb on + strandat 5121.399 kb on + strandat 5121.405 kb on + strandat 5121.406 kb on + strandat 5121.406 kb on + strandat 5121.406 kb on + strandat 5121.406 kb on + strandat 5121.407 kb on - strandat 5121.408 kb on - strandat 5121.408 kb on - strandat 5121.414 kb on - strandat 5121.414 kb on - strandat 5121.414 kb on - strandat 5121.414 kb on - strandat 5121.414 kb on - strandat 5121.414 kb on - strandat 5121.414 kb on - strandat 5121.416 kb on + strandat 5121.416 kb on + strandat 5121.416 kb on + strandat 5121.416 kb on + strandat 5121.424 kb on - strandat 5121.424 kb on - strandat 5121.424 kb on - strandat 5121.424 kb on - strandat 5121.424 kb on - strandat 5121.424 kb on - strandat 5121.424 kb on - strandat 5121.424 kb on - strandat 5121.431 kb on - strandat 5121.431 kb on - strandat 5121.435 kb on + strandat 5121.435 kb on + strandat 5121.443 kb on - strandat 5121.443 kb on - strandat 5121.489 kb on - strand, within BWI76_RS24565at 5121.493 kb on - strand, within BWI76_RS24565at 5121.495 kb on + strand, within BWI76_RS24565at 5121.498 kb on - strand, within BWI76_RS24565at 5121.543 kb on - strand, within BWI76_RS24565at 5121.578 kb on + strand, within BWI76_RS24565at 5121.669 kb on - strand, within BWI76_RS24565at 5121.674 kb on - strand, within BWI76_RS24565at 5121.690 kb on - strand, within BWI76_RS24565at 5121.714 kb on + strand, within BWI76_RS24565at 5121.722 kb on - strand, within BWI76_RS24565at 5121.835 kb on - strandat 5121.872 kb on + strand, within BWI76_RS24565at 5121.874 kb on + strand, within BWI76_RS24565at 5121.874 kb on + strand, within BWI76_RS24565at 5121.910 kb on + strandat 5121.911 kb on + strandat 5121.918 kb on - strandat 5122.022 kb on - strandat 5122.241 kb on + strandat 5122.404 kb on - strand, within BWI76_RS24575at 5122.514 kb on - strand, within BWI76_RS24575at 5122.529 kb on - strandat 5122.565 kb on + strandat 5122.573 kb on - strandat 5122.697 kb on + strandat 5122.708 kb on + strandat 5122.708 kb on + strandat 5122.716 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB1 MOI 0.1
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5,120,655 - +0.5
5,120,655 - +1.1
5,120,655 - +0.6
5,120,681 - +0.9
5,120,692 + -2.7
5,120,693 + -0.9
5,120,700 - -0.1
5,120,701 - -0.7
5,120,802 - BWI76_RS24560 0.10 -0.8
5,120,843 - BWI76_RS24560 0.16 +2.0
5,120,869 - BWI76_RS24560 0.20 -0.8
5,120,869 - BWI76_RS24560 0.20 -0.2
5,120,879 - BWI76_RS24560 0.22 +0.0
5,120,922 - BWI76_RS24560 0.28 +0.4
5,120,927 - BWI76_RS24560 0.29 +0.8
5,120,927 - BWI76_RS24560 0.29 +0.6
5,120,927 - BWI76_RS24560 0.29 -0.2
5,120,934 - BWI76_RS24560 0.30 +0.3
5,120,942 - BWI76_RS24560 0.31 +2.5
5,120,957 - BWI76_RS24560 0.33 -0.6
5,121,012 + BWI76_RS24560 0.42 +1.2
5,121,014 - BWI76_RS24560 0.42 -0.4
5,121,020 - BWI76_RS24560 0.43 +0.1
5,121,020 - BWI76_RS24560 0.43 -3.0
5,121,025 - BWI76_RS24560 0.44 +2.8
5,121,025 - BWI76_RS24560 0.44 -1.1
5,121,025 - BWI76_RS24560 0.44 -0.6
5,121,034 - BWI76_RS24560 0.45 +0.7
5,121,034 - BWI76_RS24560 0.45 -3.8
5,121,055 - BWI76_RS24560 0.48 -0.9
5,121,210 - BWI76_RS24560 0.72 +0.3
5,121,220 + BWI76_RS24560 0.73 -1.6
5,121,220 + BWI76_RS24560 0.73 +0.3
5,121,222 + BWI76_RS24560 0.73 -2.5
5,121,227 + BWI76_RS24560 0.74 +0.1
5,121,228 - BWI76_RS24560 0.74 -1.5
5,121,230 - BWI76_RS24560 0.75 -0.3
5,121,230 - BWI76_RS24560 0.75 -1.1
5,121,230 - BWI76_RS24560 0.75 -0.0
5,121,230 - BWI76_RS24560 0.75 +1.4
5,121,232 + BWI76_RS24560 0.75 -1.1
5,121,232 + BWI76_RS24560 0.75 -0.6
5,121,232 + BWI76_RS24560 0.75 +1.9
5,121,232 + BWI76_RS24560 0.75 -2.1
5,121,232 + BWI76_RS24560 0.75 +0.3
5,121,232 + BWI76_RS24560 0.75 +0.2
5,121,232 + BWI76_RS24560 0.75 -0.7
5,121,232 + BWI76_RS24560 0.75 +0.1
5,121,232 + BWI76_RS24560 0.75 -2.5
5,121,235 - BWI76_RS24560 0.75 -0.3
5,121,237 + BWI76_RS24560 0.76 +0.1
5,121,237 + BWI76_RS24560 0.76 +0.7
5,121,237 + BWI76_RS24560 0.76 -0.0
5,121,237 + BWI76_RS24560 0.76 -0.4
5,121,240 - BWI76_RS24560 0.76 +0.9
5,121,240 - BWI76_RS24560 0.76 -0.0
5,121,240 - BWI76_RS24560 0.76 +1.1
5,121,240 - BWI76_RS24560 0.76 -0.0
5,121,240 - BWI76_RS24560 0.76 -1.0
5,121,240 - BWI76_RS24560 0.76 +0.6
5,121,240 - BWI76_RS24560 0.76 +1.3
5,121,240 - BWI76_RS24560 0.76 +0.6
5,121,240 - BWI76_RS24560 0.76 +3.8
5,121,240 - BWI76_RS24560 0.76 -0.8
5,121,240 - BWI76_RS24560 0.76 +1.6
5,121,240 - BWI76_RS24560 0.76 -3.2
5,121,240 - BWI76_RS24560 0.76 -2.8
5,121,241 - BWI76_RS24560 0.76 +0.3
5,121,241 - BWI76_RS24560 0.76 +2.8
5,121,241 - BWI76_RS24560 0.76 -1.5
5,121,242 + BWI76_RS24560 0.76 -2.2
5,121,251 - BWI76_RS24560 0.78 -1.7
5,121,251 - BWI76_RS24560 0.78 -1.0
5,121,251 - BWI76_RS24560 0.78 -3.0
5,121,255 - BWI76_RS24560 0.78 -0.9
5,121,302 + BWI76_RS24560 0.86 -0.4
5,121,306 - BWI76_RS24560 0.86 -0.1
5,121,306 - BWI76_RS24560 0.86 +0.5
5,121,339 + -0.1
5,121,342 - -0.8
5,121,344 + -1.1
5,121,347 - +0.0
5,121,355 - -1.4
5,121,399 + -1.5
5,121,399 + -2.1
5,121,405 + -0.4
5,121,406 + -1.1
5,121,406 + +0.2
5,121,406 + -1.1
5,121,406 + -1.0
5,121,407 - -1.6
5,121,408 - +1.2
5,121,408 - -1.1
5,121,414 - -0.6
5,121,414 - +0.2
5,121,414 - -0.4
5,121,414 - -0.0
5,121,414 - -0.6
5,121,414 - -1.2
5,121,414 - +0.9
5,121,416 + -1.8
5,121,416 + -0.8
5,121,416 + -3.2
5,121,416 + -0.1
5,121,424 - -0.8
5,121,424 - -0.4
5,121,424 - -2.6
5,121,424 - +0.1
5,121,424 - -0.8
5,121,424 - -0.1
5,121,424 - +0.5
5,121,424 - -1.4
5,121,431 - -0.9
5,121,431 - +0.4
5,121,435 + +2.1
5,121,435 + -0.5
5,121,443 - +0.5
5,121,443 - -0.4
5,121,489 - BWI76_RS24565 0.10 -2.1
5,121,493 - BWI76_RS24565 0.11 -0.5
5,121,495 + BWI76_RS24565 0.11 +1.2
5,121,498 - BWI76_RS24565 0.12 -1.6
5,121,543 - BWI76_RS24565 0.21 +0.1
5,121,578 + BWI76_RS24565 0.28 -0.8
5,121,669 - BWI76_RS24565 0.46 -1.4
5,121,674 - BWI76_RS24565 0.47 -0.4
5,121,690 - BWI76_RS24565 0.50 -2.3
5,121,714 + BWI76_RS24565 0.54 -0.2
5,121,722 - BWI76_RS24565 0.56 -1.3
5,121,835 - +2.6
5,121,872 + BWI76_RS24565 0.86 +0.2
5,121,874 + BWI76_RS24565 0.86 -0.0
5,121,874 + BWI76_RS24565 0.86 -0.0
5,121,910 + -1.0
5,121,911 + +0.3
5,121,918 - -0.4
5,122,022 - -0.9
5,122,241 + -0.3
5,122,404 - BWI76_RS24575 0.53 +1.4
5,122,514 - BWI76_RS24575 0.87 +0.9
5,122,529 - -1.8
5,122,565 + -0.7
5,122,573 - +1.9
5,122,697 + -1.9
5,122,708 + -1.0
5,122,708 + -0.6
5,122,716 - +2.2

Or see this region's nucleotide sequence