Experiment: KLEB1 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS21705 and BWI76_RS21710 are separated by 171 nucleotides BWI76_RS21710 and BWI76_RS21715 overlap by 10 nucleotides BWI76_RS21715 and BWI76_RS21720 are separated by 1 nucleotides BWI76_RS21720 and BWI76_RS21725 overlap by 52 nucleotides BWI76_RS21725 and BWI76_RS21730 are separated by 92 nucleotides
BWI76_RS21705: BWI76_RS21705 - MFS transporter, at 4,539,495 to 4,540,685
_RS21705
BWI76_RS21710: BWI76_RS21710 - nitroreductase, at 4,540,857 to 4,541,231
_RS21710
BWI76_RS21715: BWI76_RS21715 - transcriptional regulator, at 4,541,222 to 4,541,401
_RS21715
BWI76_RS21720: BWI76_RS21720 - hypothetical protein, at 4,541,403 to 4,541,645
_RS21720
BWI76_RS21725: BWI76_RS21725 - flavin reductase, at 4,541,594 to 4,542,166
_RS21725
BWI76_RS21730: BWI76_RS21730 - hypothetical protein, at 4,542,259 to 4,542,774
_RS21730
Position (kb)
4541
4542 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4540.239 kb on - strand, within BWI76_RS21705 at 4540.404 kb on - strand, within BWI76_RS21705 at 4540.503 kb on + strand, within BWI76_RS21705 at 4540.689 kb on + strand at 4540.697 kb on - strand at 4540.697 kb on - strand at 4540.707 kb on - strand at 4540.724 kb on - strand at 4540.751 kb on - strand at 4540.772 kb on + strand at 4540.772 kb on + strand at 4540.772 kb on + strand at 4540.777 kb on + strand at 4540.782 kb on + strand at 4540.815 kb on + strand at 4540.855 kb on + strand at 4540.863 kb on - strand at 4540.863 kb on - strand at 4540.863 kb on - strand at 4540.868 kb on - strand at 4540.904 kb on - strand, within BWI76_RS21710 at 4540.925 kb on + strand, within BWI76_RS21710 at 4540.926 kb on - strand, within BWI76_RS21710 at 4541.051 kb on + strand, within BWI76_RS21710 at 4541.062 kb on + strand, within BWI76_RS21710 at 4541.083 kb on + strand, within BWI76_RS21710 at 4541.159 kb on + strand, within BWI76_RS21710 at 4541.249 kb on - strand, within BWI76_RS21715 at 4541.658 kb on + strand, within BWI76_RS21725 at 4541.673 kb on - strand, within BWI76_RS21725 at 4541.797 kb on + strand, within BWI76_RS21725 at 4541.818 kb on + strand, within BWI76_RS21725 at 4541.823 kb on + strand, within BWI76_RS21725 at 4541.823 kb on + strand, within BWI76_RS21725 at 4541.836 kb on - strand, within BWI76_RS21725 at 4541.836 kb on - strand, within BWI76_RS21725 at 4541.887 kb on + strand, within BWI76_RS21725 at 4541.914 kb on - strand, within BWI76_RS21725 at 4541.917 kb on - strand, within BWI76_RS21725 at 4541.917 kb on - strand, within BWI76_RS21725 at 4541.922 kb on - strand, within BWI76_RS21725 at 4541.935 kb on + strand, within BWI76_RS21725 at 4541.953 kb on - strand, within BWI76_RS21725 at 4541.996 kb on + strand, within BWI76_RS21725 at 4541.997 kb on + strand, within BWI76_RS21725 at 4541.997 kb on + strand, within BWI76_RS21725 at 4541.997 kb on + strand, within BWI76_RS21725 at 4542.005 kb on - strand, within BWI76_RS21725 at 4542.005 kb on - strand, within BWI76_RS21725 at 4542.005 kb on - strand, within BWI76_RS21725 at 4542.021 kb on + strand, within BWI76_RS21725 at 4542.022 kb on - strand, within BWI76_RS21725 at 4542.028 kb on - strand, within BWI76_RS21725 at 4542.040 kb on + strand, within BWI76_RS21725 at 4542.054 kb on - strand, within BWI76_RS21725 at 4542.060 kb on - strand, within BWI76_RS21725 at 4542.064 kb on + strand, within BWI76_RS21725 at 4542.088 kb on - strand, within BWI76_RS21725 at 4542.117 kb on + strand at 4542.135 kb on + strand at 4542.153 kb on - strand at 4542.153 kb on - strand at 4542.213 kb on + strand at 4542.235 kb on + strand at 4542.262 kb on + strand at 4542.262 kb on + strand at 4542.270 kb on - strand at 4542.281 kb on + strand at 4542.350 kb on - strand, within BWI76_RS21730
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB1 MOI 0.1 remove 4,540,239 - BWI76_RS21705 0.62 -0.7 4,540,404 - BWI76_RS21705 0.76 -1.2 4,540,503 + BWI76_RS21705 0.85 -0.0 4,540,689 + -1.7 4,540,697 - -3.2 4,540,697 - -1.2 4,540,707 - -0.1 4,540,724 - -1.8 4,540,751 - -1.2 4,540,772 + -2.1 4,540,772 + +1.1 4,540,772 + +0.9 4,540,777 + -1.3 4,540,782 + -0.6 4,540,815 + -0.9 4,540,855 + -1.3 4,540,863 - -2.6 4,540,863 - -0.6 4,540,863 - -2.2 4,540,868 - -0.0 4,540,904 - BWI76_RS21710 0.13 +2.6 4,540,925 + BWI76_RS21710 0.18 -0.6 4,540,926 - BWI76_RS21710 0.18 -1.2 4,541,051 + BWI76_RS21710 0.52 -2.1 4,541,062 + BWI76_RS21710 0.55 -0.2 4,541,083 + BWI76_RS21710 0.60 -2.4 4,541,159 + BWI76_RS21710 0.81 -0.3 4,541,249 - BWI76_RS21715 0.15 -2.1 4,541,658 + BWI76_RS21725 0.11 -0.6 4,541,673 - BWI76_RS21725 0.14 -1.3 4,541,797 + BWI76_RS21725 0.35 +0.4 4,541,818 + BWI76_RS21725 0.39 +0.6 4,541,823 + BWI76_RS21725 0.40 -0.1 4,541,823 + BWI76_RS21725 0.40 -2.2 4,541,836 - BWI76_RS21725 0.42 -0.6 4,541,836 - BWI76_RS21725 0.42 +2.0 4,541,887 + BWI76_RS21725 0.51 +0.6 4,541,914 - BWI76_RS21725 0.56 -0.8 4,541,917 - BWI76_RS21725 0.56 -0.6 4,541,917 - BWI76_RS21725 0.56 +1.6 4,541,922 - BWI76_RS21725 0.57 -0.8 4,541,935 + BWI76_RS21725 0.60 +0.4 4,541,953 - BWI76_RS21725 0.63 -1.6 4,541,996 + BWI76_RS21725 0.70 +1.1 4,541,997 + BWI76_RS21725 0.70 -1.5 4,541,997 + BWI76_RS21725 0.70 +0.0 4,541,997 + BWI76_RS21725 0.70 +0.1 4,542,005 - BWI76_RS21725 0.72 -0.5 4,542,005 - BWI76_RS21725 0.72 -0.1 4,542,005 - BWI76_RS21725 0.72 -1.4 4,542,021 + BWI76_RS21725 0.75 -0.2 4,542,022 - BWI76_RS21725 0.75 -0.9 4,542,028 - BWI76_RS21725 0.76 -2.2 4,542,040 + BWI76_RS21725 0.78 -1.5 4,542,054 - BWI76_RS21725 0.80 -0.1 4,542,060 - BWI76_RS21725 0.81 -0.2 4,542,064 + BWI76_RS21725 0.82 -3.9 4,542,088 - BWI76_RS21725 0.86 -2.6 4,542,117 + +0.4 4,542,135 + -1.8 4,542,153 - +0.5 4,542,153 - -2.7 4,542,213 + +1.2 4,542,235 + -0.7 4,542,262 + -1.5 4,542,262 + -0.5 4,542,270 - -3.0 4,542,281 + -3.2 4,542,350 - BWI76_RS21730 0.18 -0.9
Or see this region's nucleotide sequence