Experiment: KLEB1 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS21110 and BWI76_RS21115 are separated by 208 nucleotides BWI76_RS21115 and BWI76_RS21120 are separated by 32 nucleotides BWI76_RS21120 and BWI76_RS21125 are separated by 96 nucleotides
BWI76_RS21110: BWI76_RS21110 - hypothetical protein, at 4,418,006 to 4,422,952
_RS21110
BWI76_RS21115: BWI76_RS21115 - 3-mercaptopyruvate sulfurtransferase, at 4,423,161 to 4,424,006
_RS21115
BWI76_RS21120: BWI76_RS21120 - enhanced serine sensitivity protein SseB, at 4,424,039 to 4,424,815
_RS21120
BWI76_RS21125: BWI76_RS21125 - aminopeptidase PepB, at 4,424,912 to 4,426,198
_RS21125
Position (kb)
4423
4424
4425 Strain fitness (log2 ratio)
-2
-1
0
1 at 4422.255 kb on + strand, within BWI76_RS21110 at 4422.492 kb on - strand at 4422.517 kb on - strand at 4422.519 kb on + strand at 4422.606 kb on - strand at 4422.653 kb on + strand at 4422.894 kb on + strand at 4423.226 kb on + strand at 4423.482 kb on - strand, within BWI76_RS21115 at 4423.578 kb on - strand, within BWI76_RS21115 at 4423.745 kb on + strand, within BWI76_RS21115 at 4423.980 kb on + strand at 4424.282 kb on + strand, within BWI76_RS21120 at 4424.282 kb on + strand, within BWI76_RS21120 at 4424.282 kb on + strand, within BWI76_RS21120 at 4424.290 kb on - strand, within BWI76_RS21120 at 4424.290 kb on - strand at 4424.292 kb on - strand, within BWI76_RS21120 at 4424.384 kb on - strand, within BWI76_RS21120 at 4424.529 kb on + strand, within BWI76_RS21120 at 4424.681 kb on - strand, within BWI76_RS21120
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB1 MOI 0.1 remove 4,422,255 + BWI76_RS21110 0.86 +0.3 4,422,492 - -0.4 4,422,517 - +0.6 4,422,519 + -1.4 4,422,606 - +0.9 4,422,653 + +0.5 4,422,894 + +1.1 4,423,226 + +0.8 4,423,482 - BWI76_RS21115 0.38 -0.2 4,423,578 - BWI76_RS21115 0.49 +1.7 4,423,745 + BWI76_RS21115 0.69 +0.6 4,423,980 + -0.1 4,424,282 + BWI76_RS21120 0.31 -2.0 4,424,282 + BWI76_RS21120 0.31 +1.0 4,424,282 + BWI76_RS21120 0.31 -2.4 4,424,290 - BWI76_RS21120 0.32 +1.7 4,424,290 - -0.1 4,424,292 - BWI76_RS21120 0.33 +0.4 4,424,384 - BWI76_RS21120 0.44 +1.7 4,424,529 + BWI76_RS21120 0.63 +0.3 4,424,681 - BWI76_RS21120 0.83 +0.8
Or see this region's nucleotide sequence