Experiment: KLEB1 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20325 and BWI76_RS20330 overlap by 4 nucleotides BWI76_RS20330 and BWI76_RS20335 are separated by 66 nucleotides BWI76_RS20335 and BWI76_RS20340 are separated by 257 nucleotides
BWI76_RS20325: BWI76_RS20325 - histidine/lysine/arginine/ornithine ABC transporter permease HisM, at 4,255,553 to 4,256,269
_RS20325
BWI76_RS20330: BWI76_RS20330 - histidine/lysine/arginine/ornithine ABC transporter permease HisQ, at 4,256,266 to 4,256,952
_RS20330
BWI76_RS20335: BWI76_RS20335 - zinc transporter, at 4,257,019 to 4,257,801
_RS20335
BWI76_RS20340: BWI76_RS20340 - histidine ABC transporter substrate-binding protein HisJ, at 4,258,059 to 4,258,841
_RS20340
Position (kb) 4257
4258 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 4256.054 kb on + strand, within BWI76_RS20325 at 4256.074 kb on + strand, within BWI76_RS20325 at 4256.271 kb on + strand at 4256.302 kb on - strand at 4256.322 kb on - strand at 4256.360 kb on + strand, within BWI76_RS20330 at 4256.425 kb on - strand, within BWI76_RS20330 at 4256.449 kb on + strand, within BWI76_RS20330 at 4256.722 kb on + strand, within BWI76_RS20330 at 4256.802 kb on - strand, within BWI76_RS20330 at 4256.898 kb on + strand at 4256.898 kb on + strand at 4256.906 kb on - strand at 4257.586 kb on - strand, within BWI76_RS20335 at 4257.598 kb on + strand, within BWI76_RS20335 at 4257.680 kb on + strand, within BWI76_RS20335 at 4257.705 kb on - strand, within BWI76_RS20335 at 4257.814 kb on - strand at 4258.239 kb on + strand, within BWI76_RS20340 at 4258.417 kb on - strand, within BWI76_RS20340 at 4258.419 kb on + strand, within BWI76_RS20340
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB1 MOI 0.1 remove 4,256,054 + BWI76_RS20325 0.70 -0.7 4,256,074 + BWI76_RS20325 0.73 +0.3 4,256,271 + -0.1 4,256,302 - -2.6 4,256,322 - -1.3 4,256,360 + BWI76_RS20330 0.14 -0.5 4,256,425 - BWI76_RS20330 0.23 -2.8 4,256,449 + BWI76_RS20330 0.27 +0.6 4,256,722 + BWI76_RS20330 0.66 +0.6 4,256,802 - BWI76_RS20330 0.78 -0.4 4,256,898 + -0.6 4,256,898 + -1.0 4,256,906 - -3.1 4,257,586 - BWI76_RS20335 0.72 -0.6 4,257,598 + BWI76_RS20335 0.74 -3.0 4,257,680 + BWI76_RS20335 0.84 -2.0 4,257,705 - BWI76_RS20335 0.88 -1.7 4,257,814 - -1.4 4,258,239 + BWI76_RS20340 0.23 -0.3 4,258,417 - BWI76_RS20340 0.46 -4.7 4,258,419 + BWI76_RS20340 0.46 -0.1
Or see this region's nucleotide sequence