Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS18470

Experiment: KLEB1 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS18460 and BWI76_RS18465 are separated by 100 nucleotidesBWI76_RS18465 and BWI76_RS18470 are separated by 200 nucleotidesBWI76_RS18470 and BWI76_RS18475 are separated by 71 nucleotidesBWI76_RS18475 and BWI76_RS18480 are separated by 140 nucleotides BWI76_RS18460: BWI76_RS18460 - DgsA anti-repressor MtfA, at 3,829,916 to 3,830,713 _RS18460 BWI76_RS18465: BWI76_RS18465 - tRNA-Asn, at 3,830,814 to 3,830,889 _RS18465 BWI76_RS18470: BWI76_RS18470 - hypothetical protein, at 3,831,090 to 3,831,522 _RS18470 BWI76_RS18475: BWI76_RS18475 - hypothetical protein, at 3,831,594 to 3,831,983 _RS18475 BWI76_RS18480: BWI76_RS18480 - LysR family transcriptional regulator, at 3,832,124 to 3,833,044 _RS18480 Position (kb) 3831 3832Strain fitness (log2 ratio) -3 -2 -1 0 1at 3830.091 kb on + strand, within BWI76_RS18460at 3830.099 kb on + strand, within BWI76_RS18460at 3830.188 kb on + strand, within BWI76_RS18460at 3830.188 kb on + strand, within BWI76_RS18460at 3830.278 kb on - strand, within BWI76_RS18460at 3830.315 kb on + strand, within BWI76_RS18460at 3830.425 kb on + strand, within BWI76_RS18460at 3830.996 kb on - strandat 3830.996 kb on - strandat 3831.021 kb on + strandat 3831.061 kb on - strandat 3831.077 kb on + strandat 3831.162 kb on - strand, within BWI76_RS18470at 3831.284 kb on - strand, within BWI76_RS18470at 3831.330 kb on + strand, within BWI76_RS18470at 3831.369 kb on - strand, within BWI76_RS18470at 3831.592 kb on + strandat 3831.705 kb on + strand, within BWI76_RS18475at 3831.747 kb on + strand, within BWI76_RS18475at 3831.754 kb on + strand, within BWI76_RS18475at 3831.789 kb on + strand, within BWI76_RS18475at 3831.807 kb on - strand, within BWI76_RS18475at 3831.820 kb on + strand, within BWI76_RS18475at 3831.828 kb on - strand, within BWI76_RS18475at 3831.868 kb on + strand, within BWI76_RS18475at 3831.935 kb on + strand, within BWI76_RS18475at 3831.945 kb on + strandat 3831.984 kb on + strandat 3832.003 kb on + strandat 3832.083 kb on + strandat 3832.197 kb on + strandat 3832.220 kb on - strand, within BWI76_RS18480at 3832.375 kb on - strand, within BWI76_RS18480at 3832.409 kb on - strand, within BWI76_RS18480at 3832.434 kb on + strand, within BWI76_RS18480

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB1 MOI 0.1
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3,830,091 + BWI76_RS18460 0.22 -0.8
3,830,099 + BWI76_RS18460 0.23 -2.9
3,830,188 + BWI76_RS18460 0.34 -1.1
3,830,188 + BWI76_RS18460 0.34 -0.4
3,830,278 - BWI76_RS18460 0.45 +0.2
3,830,315 + BWI76_RS18460 0.50 +0.3
3,830,425 + BWI76_RS18460 0.64 -3.2
3,830,996 - +0.3
3,830,996 - +0.2
3,831,021 + -1.0
3,831,061 - -0.1
3,831,077 + -0.4
3,831,162 - BWI76_RS18470 0.17 -3.3
3,831,284 - BWI76_RS18470 0.45 -1.4
3,831,330 + BWI76_RS18470 0.55 +0.3
3,831,369 - BWI76_RS18470 0.64 -3.6
3,831,592 + -0.6
3,831,705 + BWI76_RS18475 0.28 +0.8
3,831,747 + BWI76_RS18475 0.39 -0.4
3,831,754 + BWI76_RS18475 0.41 -0.8
3,831,789 + BWI76_RS18475 0.50 -1.0
3,831,807 - BWI76_RS18475 0.55 -0.7
3,831,820 + BWI76_RS18475 0.58 -3.2
3,831,828 - BWI76_RS18475 0.60 -1.2
3,831,868 + BWI76_RS18475 0.70 +1.4
3,831,935 + BWI76_RS18475 0.87 +1.2
3,831,945 + -0.2
3,831,984 + +0.2
3,832,003 + -0.8
3,832,083 + +1.6
3,832,197 + +0.3
3,832,220 - BWI76_RS18480 0.10 -1.7
3,832,375 - BWI76_RS18480 0.27 +0.1
3,832,409 - BWI76_RS18480 0.31 -0.6
3,832,434 + BWI76_RS18480 0.34 -1.2

Or see this region's nucleotide sequence