Experiment: KLEB1 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17885 and BWI76_RS17890 are separated by 446 nucleotides BWI76_RS17890 and BWI76_RS17895 are separated by 60 nucleotides BWI76_RS17895 and BWI76_RS17900 are separated by 12 nucleotides
BWI76_RS17885: BWI76_RS17885 - hypothetical protein, at 3,719,573 to 3,721,132
_RS17885
BWI76_RS17890: BWI76_RS17890 - PTS mannose transporter subunit EIIAB, at 3,721,579 to 3,722,547
_RS17890
BWI76_RS17895: BWI76_RS17895 - PTS mannose/fructose/sorbose transporter subunit IIC, at 3,722,608 to 3,723,408
_RS17895
BWI76_RS17900: BWI76_RS17900 - PTS mannose transporter subunit IID, at 3,723,421 to 3,724,272
_RS17900
Position (kb)
3721
3722
3723 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3720.615 kb on + strand, within BWI76_RS17885 at 3720.786 kb on + strand, within BWI76_RS17885 at 3721.179 kb on - strand at 3721.263 kb on + strand at 3721.276 kb on + strand at 3721.282 kb on + strand at 3721.375 kb on - strand at 3721.460 kb on - strand at 3721.487 kb on + strand at 3721.535 kb on + strand at 3721.543 kb on - strand at 3721.543 kb on - strand at 3721.571 kb on + strand at 3721.700 kb on + strand, within BWI76_RS17890 at 3721.880 kb on + strand, within BWI76_RS17890 at 3722.048 kb on + strand, within BWI76_RS17890 at 3722.346 kb on - strand, within BWI76_RS17890 at 3722.471 kb on + strand at 3722.471 kb on + strand at 3722.477 kb on + strand at 3722.479 kb on - strand at 3722.640 kb on + strand at 3722.711 kb on + strand, within BWI76_RS17895 at 3722.719 kb on - strand, within BWI76_RS17895 at 3722.862 kb on - strand, within BWI76_RS17895 at 3723.115 kb on - strand, within BWI76_RS17895 at 3723.125 kb on - strand at 3723.311 kb on - strand, within BWI76_RS17895 at 3723.339 kb on + strand at 3723.358 kb on + strand at 3723.405 kb on - strand at 3723.491 kb on + strand at 3723.501 kb on + strand at 3723.542 kb on - strand, within BWI76_RS17900
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB1 MOI 0.1 remove 3,720,615 + BWI76_RS17885 0.67 +0.9 3,720,786 + BWI76_RS17885 0.78 +0.7 3,721,179 - -0.1 3,721,263 + -2.1 3,721,276 + -1.2 3,721,282 + +0.6 3,721,375 - -3.3 3,721,460 - -0.5 3,721,487 + -0.1 3,721,535 + -0.2 3,721,543 - -1.5 3,721,543 - -0.9 3,721,571 + -0.1 3,721,700 + BWI76_RS17890 0.12 -1.2 3,721,880 + BWI76_RS17890 0.31 -0.5 3,722,048 + BWI76_RS17890 0.48 -1.1 3,722,346 - BWI76_RS17890 0.79 -1.7 3,722,471 + -0.4 3,722,471 + -0.9 3,722,477 + +0.3 3,722,479 - -0.2 3,722,640 + -0.0 3,722,711 + BWI76_RS17895 0.13 -1.6 3,722,719 - BWI76_RS17895 0.14 +0.2 3,722,862 - BWI76_RS17895 0.32 -1.4 3,723,115 - BWI76_RS17895 0.63 +0.5 3,723,125 - +2.1 3,723,311 - BWI76_RS17895 0.88 -0.5 3,723,339 + -0.6 3,723,358 + -0.2 3,723,405 - -0.6 3,723,491 + +0.7 3,723,501 + -1.1 3,723,542 - BWI76_RS17900 0.14 +0.6
Or see this region's nucleotide sequence