Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS16510

Experiment: KLEB1 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS16505 and BWI76_RS16510 are separated by 551 nucleotidesBWI76_RS16510 and BWI76_RS16515 are separated by 18 nucleotides BWI76_RS16505: BWI76_RS16505 - MFS transporter, at 3,420,784 to 3,421,950 _RS16505 BWI76_RS16510: BWI76_RS16510 - transcriptional repressor PurR, at 3,422,502 to 3,423,527 _RS16510 BWI76_RS16515: BWI76_RS16515 - LysR family transcriptional regulator, at 3,423,546 to 3,424,457 _RS16515 Position (kb) 3422 3423 3424Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 3421.570 kb on - strand, within BWI76_RS16505at 3421.632 kb on + strand, within BWI76_RS16505at 3421.640 kb on - strand, within BWI76_RS16505at 3422.104 kb on - strandat 3422.139 kb on - strandat 3422.385 kb on + strandat 3422.404 kb on + strandat 3422.509 kb on + strandat 3422.765 kb on + strand, within BWI76_RS16510at 3422.766 kb on + strand, within BWI76_RS16510at 3422.766 kb on + strand, within BWI76_RS16510at 3423.615 kb on - strandat 3423.615 kb on - strandat 3423.853 kb on + strand, within BWI76_RS16515at 3424.076 kb on + strand, within BWI76_RS16515at 3424.390 kb on - strandat 3424.511 kb on + strandat 3424.518 kb on - strandat 3424.519 kb on - strandat 3424.519 kb on - strandat 3424.523 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB1 MOI 0.1
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3,421,570 - BWI76_RS16505 0.67 +0.6
3,421,632 + BWI76_RS16505 0.73 +1.8
3,421,640 - BWI76_RS16505 0.73 -3.8
3,422,104 - -1.1
3,422,139 - -2.4
3,422,385 + -0.1
3,422,404 + -0.1
3,422,509 + -2.0
3,422,765 + BWI76_RS16510 0.26 -2.0
3,422,766 + BWI76_RS16510 0.26 +0.1
3,422,766 + BWI76_RS16510 0.26 -4.1
3,423,615 - -1.3
3,423,615 - -0.1
3,423,853 + BWI76_RS16515 0.34 -0.7
3,424,076 + BWI76_RS16515 0.58 -0.1
3,424,390 - +1.4
3,424,511 + -1.5
3,424,518 - -0.2
3,424,519 - -0.3
3,424,519 - -0.3
3,424,523 + +1.0

Or see this region's nucleotide sequence