Experiment: KLEB1 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16190 and BWI76_RS16195 are separated by 127 nucleotides BWI76_RS16195 and BWI76_RS16200 are separated by 145 nucleotides BWI76_RS16200 and BWI76_RS16205 are separated by 10 nucleotides
BWI76_RS16190: BWI76_RS16190 - universal stress protein UspE, at 3,359,475 to 3,360,425
_RS16190
BWI76_RS16195: BWI76_RS16195 - hypothetical protein, at 3,360,553 to 3,360,741
_RS16195
BWI76_RS16200: BWI76_RS16200 - NAD(P) transhydrogenase subunit beta, at 3,360,887 to 3,362,275
_RS16200
BWI76_RS16205: BWI76_RS16205 - NAD(P) transhydrogenase subunit alpha, at 3,362,286 to 3,363,815
_RS16205
Position (kb)
3360
3361
3362
3363 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3360.571 kb on - strand at 3360.590 kb on + strand, within BWI76_RS16195 at 3360.681 kb on - strand, within BWI76_RS16195 at 3360.892 kb on - strand at 3361.117 kb on + strand, within BWI76_RS16200 at 3361.714 kb on + strand, within BWI76_RS16200 at 3361.912 kb on + strand, within BWI76_RS16200 at 3361.920 kb on - strand, within BWI76_RS16200 at 3362.174 kb on - strand at 3362.182 kb on + strand at 3362.246 kb on + strand at 3362.250 kb on - strand at 3362.283 kb on + strand at 3362.291 kb on - strand at 3362.404 kb on + strand at 3362.457 kb on + strand, within BWI76_RS16205 at 3362.797 kb on + strand, within BWI76_RS16205 at 3363.199 kb on + strand, within BWI76_RS16205
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB1 MOI 0.1 remove 3,360,571 - +0.2 3,360,590 + BWI76_RS16195 0.20 -0.1 3,360,681 - BWI76_RS16195 0.68 -0.5 3,360,892 - +0.6 3,361,117 + BWI76_RS16200 0.17 -2.7 3,361,714 + BWI76_RS16200 0.60 -0.2 3,361,912 + BWI76_RS16200 0.74 -1.4 3,361,920 - BWI76_RS16200 0.74 -3.6 3,362,174 - +0.6 3,362,182 + -0.8 3,362,246 + -1.2 3,362,250 - +0.2 3,362,283 + -0.9 3,362,291 - -1.2 3,362,404 + -0.4 3,362,457 + BWI76_RS16205 0.11 -0.9 3,362,797 + BWI76_RS16205 0.33 -0.5 3,363,199 + BWI76_RS16205 0.60 +0.2
Or see this region's nucleotide sequence