Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS15575

Experiment: KLEB1 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS15565 and BWI76_RS15570 are separated by 78 nucleotidesBWI76_RS15570 and BWI76_RS15575 are separated by 67 nucleotidesBWI76_RS15575 and BWI76_RS15580 overlap by 11 nucleotidesBWI76_RS15580 and BWI76_RS15585 are separated by 112 nucleotidesBWI76_RS15585 and BWI76_RS15590 are separated by 182 nucleotides BWI76_RS15565: BWI76_RS15565 - hypothetical protein, at 3,260,578 to 3,260,790 _RS15565 BWI76_RS15570: BWI76_RS15570 - phage antirepressor Ant, at 3,260,869 to 3,261,642 _RS15570 BWI76_RS15575: BWI76_RS15575 - hypothetical protein, at 3,261,710 to 3,262,429 _RS15575 BWI76_RS15580: BWI76_RS15580 - putative transcriptional repressor arc (modular protein), at 3,262,419 to 3,262,592 _RS15580 BWI76_RS15585: BWI76_RS15585 - DNA-binding protein, at 3,262,705 to 3,263,067 _RS15585 BWI76_RS15590: BWI76_RS15590 - hypothetical protein, at 3,263,250 to 3,263,957 _RS15590 Position (kb) 3261 3262 3263Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 3260.741 kb on - strand, within BWI76_RS15565at 3260.741 kb on - strand, within BWI76_RS15565at 3260.842 kb on - strandat 3260.854 kb on + strandat 3260.867 kb on + strandat 3260.875 kb on - strandat 3260.929 kb on - strandat 3261.039 kb on - strand, within BWI76_RS15570at 3261.283 kb on - strand, within BWI76_RS15570at 3261.526 kb on + strand, within BWI76_RS15570at 3261.534 kb on - strand, within BWI76_RS15570at 3261.747 kb on - strandat 3261.755 kb on + strandat 3262.170 kb on - strand, within BWI76_RS15575at 3262.267 kb on + strand, within BWI76_RS15575at 3262.308 kb on - strand, within BWI76_RS15575at 3262.338 kb on - strand, within BWI76_RS15575at 3262.341 kb on - strand, within BWI76_RS15575at 3262.400 kb on + strandat 3262.408 kb on - strandat 3262.702 kb on + strandat 3263.301 kb on + strandat 3263.403 kb on + strand, within BWI76_RS15590

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB1 MOI 0.1
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3,260,741 - BWI76_RS15565 0.77 +0.1
3,260,741 - BWI76_RS15565 0.77 -1.3
3,260,842 - -3.0
3,260,854 + -1.5
3,260,867 + -1.3
3,260,875 - -0.1
3,260,929 - -1.8
3,261,039 - BWI76_RS15570 0.22 -1.0
3,261,283 - BWI76_RS15570 0.53 -0.9
3,261,526 + BWI76_RS15570 0.85 -3.9
3,261,534 - BWI76_RS15570 0.86 -1.3
3,261,747 - -0.9
3,261,755 + -1.9
3,262,170 - BWI76_RS15575 0.64 -1.7
3,262,267 + BWI76_RS15575 0.77 -1.3
3,262,308 - BWI76_RS15575 0.83 -1.0
3,262,338 - BWI76_RS15575 0.87 -3.8
3,262,341 - BWI76_RS15575 0.88 -1.3
3,262,400 + +1.4
3,262,408 - +1.0
3,262,702 + -2.6
3,263,301 + -1.0
3,263,403 + BWI76_RS15590 0.22 -0.4

Or see this region's nucleotide sequence