Experiment: KLEB1 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13015 and BWI76_RS13020 are separated by 96 nucleotides BWI76_RS13020 and BWI76_RS13025 are separated by 137 nucleotides BWI76_RS13025 and BWI76_RS13030 are separated by 214 nucleotides
BWI76_RS13015: BWI76_RS13015 - LysR family transcriptional regulator, at 2,715,824 to 2,716,735
_RS13015
BWI76_RS13020: BWI76_RS13020 - NmrA family protein, at 2,716,832 to 2,717,821
_RS13020
BWI76_RS13025: BWI76_RS13025 - histidine phosphatase family protein, at 2,717,959 to 2,718,579
_RS13025
BWI76_RS13030: BWI76_RS13030 - site-specific recombinase, phage integrase family protein, at 2,718,794 to 2,719,396
_RS13030
Position (kb)
2716
2717
2718 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2715.900 kb on + strand at 2715.905 kb on + strand at 2715.908 kb on - strand at 2715.910 kb on + strand at 2715.918 kb on - strand, within BWI76_RS13015 at 2715.974 kb on - strand, within BWI76_RS13015 at 2715.979 kb on - strand, within BWI76_RS13015 at 2716.020 kb on - strand, within BWI76_RS13015 at 2716.046 kb on - strand, within BWI76_RS13015 at 2716.054 kb on + strand, within BWI76_RS13015 at 2716.238 kb on - strand, within BWI76_RS13015 at 2716.688 kb on - strand at 2716.728 kb on - strand at 2717.120 kb on + strand, within BWI76_RS13020 at 2717.144 kb on - strand, within BWI76_RS13020 at 2717.359 kb on + strand, within BWI76_RS13020 at 2717.457 kb on - strand, within BWI76_RS13020 at 2717.654 kb on + strand, within BWI76_RS13020 at 2717.774 kb on + strand at 2718.049 kb on + strand, within BWI76_RS13025 at 2718.294 kb on + strand, within BWI76_RS13025 at 2718.419 kb on + strand, within BWI76_RS13025 at 2718.503 kb on + strand, within BWI76_RS13025 at 2718.633 kb on + strand at 2718.776 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB1 MOI 0.1 remove 2,715,900 + -3.0 2,715,905 + +1.0 2,715,908 - -4.7 2,715,910 + -0.8 2,715,918 - BWI76_RS13015 0.10 -1.1 2,715,974 - BWI76_RS13015 0.16 -2.2 2,715,979 - BWI76_RS13015 0.17 -1.7 2,716,020 - BWI76_RS13015 0.21 -0.0 2,716,046 - BWI76_RS13015 0.24 -0.2 2,716,054 + BWI76_RS13015 0.25 -0.6 2,716,238 - BWI76_RS13015 0.45 +0.2 2,716,688 - +0.0 2,716,728 - +0.1 2,717,120 + BWI76_RS13020 0.29 -0.4 2,717,144 - BWI76_RS13020 0.32 -0.6 2,717,359 + BWI76_RS13020 0.53 -0.0 2,717,457 - BWI76_RS13020 0.63 -1.6 2,717,654 + BWI76_RS13020 0.83 +0.1 2,717,774 + -4.0 2,718,049 + BWI76_RS13025 0.14 +2.9 2,718,294 + BWI76_RS13025 0.54 -0.4 2,718,419 + BWI76_RS13025 0.74 +0.9 2,718,503 + BWI76_RS13025 0.88 +0.9 2,718,633 + -0.8 2,718,776 + +1.4
Or see this region's nucleotide sequence